Anxiety & Depression Aron Test for Screen Anxiolytic Substances Anxiety & Depression • Aron Test for Screen Anxiolytic Substances Specifications cage Materials Dimensions (H x W x D) shock shock Delivery white, transparent plastic and stainless steel 16 x 25 x 18 cm (6.3 x 9.8 x 7 in) 0-3 ma, timer 0-10 sec, square pulse footswitch Ordering Information Order # 76-0006 Model Le830* Product aron test box * shock generator (76-0159) must be ordered separately. RefeRences key features } An elegant and economical solution for screening anxiolytic drugs in mice } Punishment based conflict test } Shock with adjustable intensity parameters measured Andreasen JT et al. (2013) A combined ⁸7 nicotinic acetylcholine receptor agonist and monoamine reuptake inhibitor, NS9775, represents a novel profile with potential benefits in emotional and cogniHYPERLINK tive disturbances. Neuropharmacology, Volume 73, October 2013, Pages 183–191. Chlon-Rzepa G et al. (2013) New 8-aminoalkyl derivatives of purine-2,6-dione with arylalkyl, allyl or propynyl substituents in position 7, their 5-HT1A, 5-HT2A, and 5-HT7 receptor affinity and pharmacological evaluation. Pharmacological Reports 65: 1529. Hache G et al. (2012) Antinociceptive effects of fluoxetine in a mouse modelof anxiety/ depression. NeuroReport 2012, 23:525–529. Bourin M et al. (2010) Implication of 5-HT2 receptor subtypes in the mechanism of action of the GABAergic compound etifoxine in the ”four-plate” test in Swiss mice. Behavioural Brain Res. 208(2):352-358. Foreman MM et al. (2009) Anxiolytic effects of lamotrigine and JZP-4 in the elevated plus maze and in the four plate conflict test. Eur J Pharmacol. 602(2-3):316-20. Jacobsen JP et al. (2008) SK3 K+ channel-deficient mice have enhanced dopamine and serotonin release and altered emotional behaviors. Genes Brain Behav. 7(8):836-48. Masse F et al. (2008) Anxiolytic-like effects of DOI microinjections into the hippocampus (but not the amygdala nor the PAG) in the mice four plates test. Behav. Brain Res. 188(2):291-297. } Number of punished crossings components included } Aron box } Control unit footswitch } Cables and connectors } Instruction manual } 2 year warranty The Aron Test, or Four Plates Test, is an animal model of anxiety in which the exploration of the novel surroundings is suppressed by the delivery of a mild electric foot shock. The apparatus consists of a cage floor with four identical rectangular metal plates (8 x 11 cm) (3 x 4 in) separated from one another by a gap of 4 mm (0.2 in). The plates are connected to a shocker unit that can generate electric foot shocks. Following the habituation period, the animal is subjected to an electric shock when crossing (transition) from one plate to another, i.e. two legs on one plate and two legs on another. Boissier et al. 1968 described this test first. The number of punished crossings is generally calculated for a period of 60 seconds. A substance with anxiolytic properties induces an increase in the number of punished passages. 76 www.coulbourn.com • www.panlab.com