Circadian Biology Big Brother Video Based Tracking for Circadian Studies Big Brother is a video-based activity monitor for behavioral neuroscience, neuropharmacology and circadian biology applications. Big Brother tracks the distance travelled by each monitored animal via our USB cameras. The resulting data can be analyzed and viewed in the analysis program. For long-term monitoring of circadian biology, records can be imported into ClockLab, the standard analysis program for the field of circadian biology analysis. The system can record up to 200 animals easily using up to 4 cameras. Up to 50 animals can be tracked by each camera. Event markers for time of drug application, environmental changes, etc. can be applied independently for each channel or all channels can be marked at once. Big Brother stores the complete frame-by-frame path of each animal for detecting stereotype and other behaviors. The image on the camera can be divided into any number of zones. The animal in each zone is tracked independently for maximum flexibility. The Big Brother system has been used to track animals from Drosophila to zebra fish to mice. With superior temporal resolution, Big Brother locates each animal up to 4 times per second, depending on the number of animals being tracked, and calculates the distanced travelled compared to the previous location. The distance is added to the cumulative total for the current minute and after each minute, the total distance is recorded and reset to zero. Our cameras are infrared sensitive so tracking is possible and uniform under visible, infrared or on a light-dark cycle where lighting changes from one to the other during the testing period. Data analysis is simple as the data exports to a spreadsheet file or directly to an open Excel worksheet multiple analyses for all the animals in a file. For circadian analysis, ClockLab Analysis also features numerous batch analysis features. Circadian Biology • Big Brother Video Based Tracking for Circadian Studies Ordering Information key features Order # AcT-400 AcT-711 Product big brother Video based tracking Software, up to 200 subjects uSb interface } Easy setup and operation for tracking up to 200 animals } Event markers and path tracking for stereotypy and detection of other behaviors of interest AcT-VP-02 uSb camera Kit, includes Lens, mounting bracket and cabling } Extremely flexible } High temporal resolution, locating each subject up to four times per second note see our other products that can be used for circadian Applications: • Rodent Activity Wheel, page 27 • OxyletPro, pages 88-89 } Infrared sensitive for recording where light levels vary } Extensive batch analysis 30 www.coulbourn.com • www.panlab.com