Analgesia Thermal Place Preference This behavioral assay will allow monitoring of temperature preferences, nociceptive thresholds and investigate the role of a given gene or compound on these thresholds. As advised by A. Moqrich and published in Moqrich et al (Science 2005, 307: 1468-72), this test allows researchers to work on unrestrained animals whom are free to choose their preferred position between two compartments at different temperatures. Completely investigator-independent, the Two Temperature Choice Test elicits a nociceptive response from the subject. The obtained value is a temperature or temperature range indicating the sensitivity of the subject. Using automatic detection software (optional), the user sets the temperature of each zone, defines a protocol of temperature changes or ramps, and starts the measurement process. Two animals can be observed simultaneously and independently, making the system remarkable efficient. The animals are video tracked and the software records their position vs. temperature and time. When the optional automatic detection software is not used, temperatures must be defined manually and the user must measure the time and animal position. key features Analgesia • Thermal Place Preference Specifications Temperature Range Temperature Accuracy Power supply Dimensions (L x W x H) Animal cage Animal cage Material -3°c to +65°c (room temperature 20 to 25°c) ± 0.5°c 150 watts, 120/240 Vac 32 x 57 x 45.5 cm (12.6 x 22.4 x 17.9 in) including cage 330 x 165 x 300 mm (13 x 6.5 x 11.8 in) clear plexiglass } Easily monitor thermal place preference and nociceptive thresholds } Unrestrained animals allows for maximum accuracy } Optional Automatic Detection Software eliminates the user subjectivity by establishing an automatic response parameters measured } Time spent in each zone } Time of zone trespassing } Temperature of each zone options Ordering Information Order # 76-0475 76-0476 RefeRences Descoeur J, Pereira V, Pizzoccaro A, Francois A, Ling B, Maffre V, Couette B, Busserolles J, Courteix C, Noel J, Lazdunski M, Eschalier A, Authier N, Bourinet E. Oxaliplatin-induced cold hypersensitivity is due to remodeling of ion channel expression in nociceptors. EMBO J. Mol Med 2011 May; 3(5):266-78. Mishra S, Tisel S, Orestes P, Bhangoo S, Hoon M. TRPV1-lineage neurons are required for thermal sensation. EMBO J. 2011 February; 30, 582-593. Model biot2ct bioDHcPSoft Product thermal Place Preference thermal Place Preference Software } Automatic detection software – which includes tripod, 3 USB cables and USB Camera www.panlab.com • www.coulbourn.com 43