Video Tracking During the recording of a trial, LimeLight allows you to save the images collected for viewing or conversion to a QuickTime movie. QuickTime movie formatted segments can be imported into PowerPoint or other presentation software. The LimeLight Analysis window shows a complete list of the animals contained in the data file – simply click on an animal to instantly display their motion path. If images from the trial were stored, a movie of the trial is available for viewing using the video buttons and key commands below the image. The trial analysis is also displayed, to the right, detailing the number of crossings per grid square as an intensity graph. A simple checkbox changes the display to a table format with numbers for each grid square. A pull-down menu allows the user to select from the many different analysis parameters, such as distance travelled, time in each grid square, mean length in each grid square, latency to each square, etc. A double-click on the color map exports the data to a text file and opens the file in Excel. A portion of the trial can be analyzed, rather than the full trial, by toggling the “all/part” checkbox and setting the starting and ending time for the desired portion. Scored behaviors are also shown in the analysis widow to the lower right; double clicking the graph produces a more detailed analysis. Grids can be adjusted or custom zones can be created from arbitrary groups of grid squares by double-clicking on the image. Separate windows are accessible for viewing distance and speed against time, mean distance to center, animal length and circling. Any selected parameter can be exported directly to an Excel spreadsheet for instant viewing and statistical analysis. Tracking can be based on body (center of mass, nose, or tail) via pull-down menu. Complex analyses such as animal length, speed and distance against time, mean distance to center and more are available via the Analysis screen. Users can easily divide all the trials stored into a number of groups with the Groups Window. Once groups are specified, batch exporting of zone and grid data to Excel is easy with a single command. Parameters included in batch analysis are: • Grid or Zone crossings • Distance travelled • Time spent in grid or zone • Mean length of the animal • % time animal spent in a particular length range • Animal orientation Options for batch analysis allow: • Selection of a portion of each trial for analysis - user specifies the analysis start and end time relative to the trial start and end • Division of the trials into multiple intervals for analysis • Divide trials into named groups for subsequent analysis to compile mean and standard error for each group Ordering Information Order # AcT-300A AcT-300A-U AcT-711 AcT-VP-02 U90-04 Product LimeLight Video tracking Software LimeLight upgrade to Version 3 Video Tracking Accessories for use with LimeLight actimetrics uSb interface uSb camera Kit (includes lens, mounting bracket and cabling) uSb 2.0 mini 4-Port Hub note RefeRences: We offer a full line of Open field chambers & Mazes for use with our Tracking systems. contact us for more information. • LimeLight Video Tracking Software Wagnon, J.L., Korn, M.J., Parent, R., Tarpey, T.A., Jones, J.M., Hammer, M.F., Murphy, G.G., Parent, J.M., and Meisler, M.H. (2015). Convulsive seizures and SUDEP in a mouse model of SCN8A epileptic encephalopathy. Human molecular genetics 24, 506-515. Dias, B.G., Goodman, J.V., Ahluwalia, R., Easton, A.E., Andero, R., and Ressler, K.J. (2014). Amygdala-dependent fear memory consolidation via miR-34a and Notch signaling. Neuron 83, 906-918. Ohmura, Y., Tanaka, K.F., Tsunematsu, T., Yamanaka, A., and Yoshioka, M. (2014). Optogenetic activation of serotonergic neurons enhances anxiety-like behaviour in mice. The international journal of neuropsychopharmacology / official scientific journal of the Collegium Internationale Neuropsychopharmacologicum 17, 1777-1783. Mastwal, S., Ye, Y., Ren, M., Jimenez, D.V., Martinowich, K., Gerfen, C.R., and Wang, K.H. (2014). Phasic dopamine neuron activity elicits unique mesofrontal plasticity in adolescence. The Journal of neuroscience : the official journal of the Society for Neuroscience 34, 9484-9496. Dewan A, Pacifico R, Zhan R, Rinberg D, Bozza T. (2013) Non-redundant coding of aversive odours in the main olfactory pathway. Nature. 497:486-489. Timothy P. O’Leary and Richard E. Brown (2013) Optimization of apparatus design and behavioral measures for the assessment of visuo-spatial learning and memory of mice on the Barnes maze. Learning and Memory 20: 85-96. Harris AP, Holmes MC, de Kloet ER, Chapman KE, Seckl JR (2013) Mineralocorticoid and glucocorticoid receptor balance in control of HPA axis and behaviour. Psychoneuroendocrinology 38:648-58. Moore SJ, Deshpande K, Stinnett GS, Seasholtz AF, Murphy GG. (2013) Conversion of short-term to long-term memory in the novel object recognition paradigm. Neurobiology of Learning and Memory 105:174-85. Francisco J. Monje, Isabella Divisch, Marvie Demit, Gert Lubec & Daniela D. Pollak (2013) Flotillin-1 is an evolutionary-conserved memory-related protein up-regulated in implicit and explicit learning paradigms Annals of Medicine 45:301-307. www.panlab.com • www.coulbourn.com 11