Analgesia Hot-Plate for Evaluating Thermal Analgesia The Hot-Plate performs rapid and precise screening of analgesic drug properties on small-laboratory animals according to the ‘hot-plate test’. The animal’s pain sensitivity alterations induced by a specific experimental context change and/or genetic manipulations can also be evaluated through this method. The hot-plate test, initially described by N.B. Eddy and D. Leimbach (1953), evaluates thermal pain reflexes due to footpad contact with a heated surface. During the experiments, the animal is confined in a removable clear acrylic cylinder where the latency time to the first hind paw or/and jumping responses are measured. The Hot-Plate features a thick aluminum plate (10 mm) provides a high temperature stability and even surface distribution. The plate temperature can be held at a set point between 45 and 62°C (± 0.1°C) by multiple proportional feedback circuits that minimize overshoot. A built-in timer activated by an external foot switch allows precise measurement of reaction time (0.1 sec precision). A remote foot-switch controls the test start/stop allowing rapid handsfree experiments. The operator can read the animal reaction time from the display or from a PC computer using SEDACOM software. Trial number, plate temperature and reaction time are then sent to the software through a RS-232 port. Analgesia • Hot-Plate for Evaluating Thermal Analgesia key features } Digital set point } Built-in electronic timer } Foot switch timing operation } New optional data transfer software, SEDACOM V2.0 parameters measured Specifications Base Dimensions (W x D x H) 200 x 300 x 110 mm (8 x 12 x 4 in) Plate Dimensions (D) cylinder Dimensions (D x H) Operating Temperature Reaction Time Material composition Power Requirements certifications 200 mm (8 in) 200 x 250 mm (8 x 10 in) 45 to 62 degrees celsius; 0.1 steps 3 digits, 0.01 sec increment clear methacrylate (animal holder), aluminum (plate) 110 or 220 Vac, 50/60 Hz ce compliant } Time latency to ‘paw licking’ } Time latency to ‘jumping’ components included } Base with heating plate } Footswitch } Cables and connectors } Certificate of calibration } Instruction manual } Set of spare fuses } 2 year warranty options Ordering Information Order # 76-0113 Options 76-0406 76-0608 RefeRences Fornaguera C et al. (2015) PLGA nanoparticles prepared by nano-emulsion templating using low-energy methods as efficient nanocarriers for drug delivery across the blood-brain barrier. J Control Release. 2015 Aug 10;211:134-43. Parvathy SS et al. (2015) Coadministration of indomethacin and minocycline attenuates established paclitaxel-induced neuropathic thermal hyperalgesia: Involvement of cannabinoid CB1 receptors. Sci Rep. 2015 Jun 18;5:10541. Model Le 7406 Product Hot-Plate thermal analgesia meter SeDacomV2.0 SeDacom V2.0 conrS232uSb rS-232/uSb adapter } SEDACOM software } RS-232/USB Adapter www.panlab.com • www.coulbourn.com 41