Learning Memory & Attention FreezeFrame Software for Fear Conditioning Learning Memory & Attention Ordering Information Order # AcT-100A AcT-100A-U AcT-110 AcT-120 AcT-130 AcT-141 Product freezeframe Software (for 1-4 subjects) freezeframe upgrade to Version 3 freezeframe Shock control expansion Kit freezeframe Light/tone control expansion Kit freezeframe fan/House Light control Kit freezeframe audio expansion Kit key features } Only video-based system for fear conditioning, a standard in the field RefeRences Ainsley, J.A., Drane, L., Jacobs, J., Kittelberger, K.A., and Reijmers, L.G. (2014). Functionally diverse dendritic mRNAs rapidly associate with ribosomes following a novel experience. Nature communications 5, 4510. Liu, C.C., Tsai, C.W., Deak, F., Rogers, J., Penuliar, M., Sung, Y.M., Maher, J.N., Fu, Y., Li, X., Xu, H., et al. (2014). Deficiency in LRP6-mediated Wnt signaling contributes to synaptic abnormalities and amyloid pathology in Alzheimer’s disease. Neuron 84, 63-77. 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Lugo, J.N., Brewster, A.L., Spencer, C.M., and Anderson, A.E. (2012). Kv4.2 knockout mice have hippocampal-dependent learning and memory deficits. Learning and Memory 19:182189. } Optimization of sensitivity with automation • } Proprietary motion detection algorithm to filter out } Superior performance in low light FreezeFrame Software for Fear Conditioning shadows, light flicker and camera noise – detecting movements as small as 1 mm FreezeFrame is the first video-based system for fear conditioning and has become the standard in its field. Combining the sensitivity of the human observer with the objectivity and throughput of automation, FreezeFrame is able to measure minute movements of grooming, sniffing, turning and rearing. The animal is monitored 2-4 times per second for data that is far more objective and reproducible. FreezeFrame can also be utilized for learned helplessness experiments, including tail suspension test and the Porsolt Forced Swim Test. FreezeFrame is not a tracking system – featuring a proprietary motion detection algorithm, the system filters out shadows, light flicker and camera noise to detect movements as small as 1 mm. FreezeFrame offers superior performance in low-light and is fully validated, with greater than 90% concordance with trained human observers. Easily store and recall multiple stimulus protocols for training and testing phases. Video images can be stored for future review and also exported as QuickTime and AVI movies for presentation. Batch export of data and analyses to Excel allows for easy analyzing of % freezing, number of bouts, bout duration and intervals for specified periods. Either white noise or pure tone can be delivered through the computer’s sound card. The FreezeFrame system accommodates multiple sounds of different amplitude, frequency and quality throughout a single trial. Stimuli – including lights, external sound generators and/or shock generators are also controlled though the computer. note Please contact your local sales and application specialist for more information on the environmental hardware and accessories available for the test cage configuration. www.panlab.com • www.coulbourn.com 71