Video Tracking WaterMaze’s analysis capabilities are unparalleled. Analyses can be viewed for single trials or exported for multiple trials directly to Excel. Scan through hundreds of trials easily with clickable animal and trial lists. Parameters include thigmotaxis, Moser’s zone, Whishaw’s corridor, Gallagher’s proximity, quadrant times and crossings and more. Suggestions for new analyses from our users are regularly incorporated. Swim paths can be exported to standard drawing programs. Movies of each trial can be stored and viewed through the WaterMaze Analysis windows or exported to PowerPoint and other presentation software. WaterMaze’s tracking is robust and easy to setup with standard CCD cameras and video boards. No special lighting or video-processing hardware is necessary. Utilizing a state-of-the-art image-processing software and videocapture card, images are acquired at up to 30 frames per second. The WaterMaze program identifies the animal whether it is lighter or darker than the background. A unique algorithm screens out changing shadows and changing lighting conditions that might occur during the trial. On-line interpolation identifies and smoothes occasional loss of signal that can occur if an animal swims briefly underwater or under hanging cue objects. A special set-up window can be used to measure the evenness of lighting across the pool surface, helping to eliminate deep shadows. The path is displayed in real time as the trial progresses. Landmarks or other cues of any color can be arranged in and around the pool, and the program can control multiple On-Demand (Atlantis) Platforms. Movies of each trial can be stored for later review or for export in QuickTime format to PowerPoint or other presentation software. At the end of each trial, the experimenter can type notes concerning the trial. The trial can be stopped manually or automatically when the animal reaches the platform or after a set interval. The program indicates the next animal, platform location and start position. Remote switches can be used to start, stop and save trials. Multiple switches for each function can be placed around the pool if desired. With the program in auto-sequence mode and autosave mode, multiple trials can be performed without a mouse or keyboard! WaterMaze goes far beyond existing programs in its ease of use and flexibility. Because it is designed in close consultation with users, WaterMaze is integrated seamlessly with the experiment. See additional information on our Circular Pool and Water Tanks on pages 54-55. Ordering Information Order # AcT-200A AcT-200A-U AcT-711 AcT-VP-02 Product watermaze Software and analysis Suite watermaze upgrade to Version 4 Video Tracking Accessories for use with WaterMaze actimetrics uSb interface uSb camera Kit (includes lens, mounting bracket and cabling) Optional Accessories AcT-200-4-D AcT-200-4-L AcT-200-6-D AcT-200-6-L AcT-201 AcT-202 AcT-204A watermaze tank 4-foot Diameter, Dark interior watermaze tank 4-foot Diameter, Light interior watermaze tank 6-foot Diameter, Dark interior watermaze tank 6-foot Diameter, Light interior watermaze Platform adjustable watermaze Platform nautilus watermaze Platform (requires an act-711 uSb interface) • WaterMaze Video Tracking Software for the Morris Water Maze note RefeRences: see pages 54-55 for more information on our circular Pools and Tanks. Lee, Y.S., Ehninger, D., Zhou, M., Oh, J.Y., Kang, M., Kwak, C., Ryu, H.H., Butz, D., Araki, T., Cai, Y., et al. (2014). Mechanism and treatment for learning and memory deficits in mouse models of Noonan syndrome. Nature neuroscience 17, 1736-1743. Richards, B.A., Xia, F., Santoro, A., Husse, J., Woodin, M.A., Josselyn, S.A., and Frankland, P.W. (2014). Patterns across multiple memories are identified over time. Nature neuroscience 17, 981-986. de Hoz, L., and Martin, S.J. (2014). Double dissociation between the contributions of the septal and temporal hippocampus to spatial learning: the role of prior experience. Hippocampus 24, 990-1005. Bruno M. da Silva, Tobias Bast, and Richard G.M. Morris (2013) Spatial memory: behavioral determinants of persistence in the watermaze delayed matching-to-place task. Learning and Memory 21: 28-36. A. S. Sengar, J. Ellegood, A. P. Yiu, H. Wang, W. Wang, S. C. Juneja, J. P. Lerch, S. A. Josselyn, R. M. Henkelman, M. W. Salter, and S. E. Egan (2103) Vertebrate Intersectin1 Is Repurposed to Facilitate Cortical Midline Connectivity and Higher Order Cognition. The Journal of Neuroscience, 33: 4055-4065. G. I. Cancino, A. P. Yiu, M. P. Fatt, C. B. Dugani1, E. R. Flores, P. W. Frankland, S. A. Josselyn,F . D. Miller, and D. R. Kaplan (2013) p63 Regulates Adult Neural Precursor and Newly Born Neuron Survival to Control Hippocampal-Dependent Behavior Journal of Neuroscience33:12569-12585. Alonso Martinez-Canabal, Katherine G. Akers, Sheena A. Josselyn, Paul W. Frankland (2013) Age-dependent effects of hippocampal neurogenesis suppression on spatial learning.Hippocampus 23:66–74. Kotermanski SE1, Johnson JW, Thiels (2013) Comparison of behavioral effects of the NMDA receptor channel blockers memantine and ketamine in rats. Pharmacology, Biochemistry and Behavior 109:67-76. www.panlab.com • www.coulbourn.com 13