Video Tracking LimeLight Video Tracking Software LimeLight can be adapted to meet the needs of a wide range of applications. With a complete range of features, this video tracking system is very easy to configure and operate to acquire meaningful data. LimeLight easily tracks up to four arenas simultaneously. A single camera can be used for multiple arenas or for greater flexibility, one camera can be used for each arena. Powerful enough to track white or black animals on any background, special lighting is not necessary. Images are collected up to 30 frames per second for a single animal or up to eight frames per second when tracking four arenas at once. Images are compressed and stored for later review and off-line scoring of complex behaviors. Video can easily be exported to AVI or QuickTime format for inclusion in presentations. key features Video Tracking • LimeLight Video Tracking Software } Developed in close collaboration with behavioral neuroscientists } Flexible and powerful video tracking system } Ideal for: Open Field, Plus Maze, Zero Maze, Novel Object Recognition, Conditioned Place Preference parameters measured Our system simplifies zone creation through dedicated interfaces for open field and elevated plus maze testing. A custom zone setup permits unlimited flexibility for more specialized applications with an easy-to-use click-and-drag interface. Zone configurations can be edited during analysis and applied to previously recorded trials. Scored behaviors can be noted by assigning a key stroke or by mouse clicking, either during the trial or video playback. Video can be played back frame-by-frame, backwards and forwards, at real-time speed or fast forward speed, with key commands for each mode. The user can opt to scroll quickly through the video by dragging the green slider beneath the image. Behaviors can be selected by clicking on the green buttons or by the key commands selected for each behavior. The user can select to preserve previous scores for some behaviors while re-analyzing to concentrate on scoring others. Up to 20 different sets of behaviors, with 16 behaviors each, can be stored and selected for use on any trial. Statistics on scored behaviors can be viewed and exported to Excel for analysis. A main analysis window provides a graphic overview for each trial. Acquired data can be exported zone-by-zone or as a summary to Excel or a text file. Data from a group of animals can be collected into a single data file for later analysis. Data handling is extremely flexible. LimeLight’s interface makes it a pleasure to use. Dozens of shortcuts make navigating the many features of the program nearly instantaneous. Most displays, for example, can be double-clicked to display more details of an analysis or to change the displayed parameters, without searching through menus for the relevant item. } Distance travelled in each region (cm or %) } Time spent in each region (sec or %) } Crossings into each region } Body length from nose to base tail (mm), together with stretch-attend bouts } Pointing direction (orientation) } Mean distance from center } Latency to zone } Distance and velocity vs. time } Statistics for scored behaviors (1st and 2nd order) } Statistics on occurrence of stimuli (output control) } Zone sequencing } Circling components included } Software } Instruction manual } Software updates of acquired system (excludes upgrades) 10 www.coulbourn.com • www.panlab.com