IH-5 Core System for Isolated Rat, Guinea Pig and Rabbit Heart

IH-5 Core System for Isolated Rat, Guinea Pig and Rabbit

The IH-5 Langendorff Core System is the starting point for all isolated perfused heart experiments for rodent models (rat, guinea pig) in the Langendorff retrograde perfusion mode.

The core Langendorff system can be easily upgraded to a working heart-ready configuration any time later.

Please Note: A fully functional IH-5 system requires the addition of core system options and other components. Other options and upgrades enable specific measurements or applications. Please see the Full Description for details.

To ensure that your system is properly configured as a functional unit that meets your application needs, please complete our Checklist and then contact Technical Services at 800-547-6766 or via email at support@hbiosci.com before placing an order. In Europe, please call +49 7665 92000 or via email at sales@hugo-sachs.de. Upgrades can also be modified to meet your specific needs.

Item# Description U.S. List Price Quantity
73-4398 IH-5 Core System for Isolated Rat, Guinea Pig or Rabbit Heart, 230 VAC
73-4397 IH-5 Core System for Isolated Rat, Guinea Pig or Rabbit Heart, 115 VAC
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The IH-5 is an isolated organ perfusion system for use with hearts of rodents (rats, guinea pigs). The flow limitation is about 500 ml/min. The perfusate is oxygenated in a 2- or 6-liter water jacketed reservoir with multiple ports for one-directional or recirculation of the perfusate. The IH-5 Langendorff Core System is the starting point for all isolated perfused heart experiments for rat, guinea pig and rodent models in the Langendorff retrograde perfusion mode.

Advanced System Design
Features & Benefits
Applications
Measured Signals & Calculated Parameters
Included items
Additional Requirements for a Functional Langendorff Unit
Additional Components
Options and Upgrades


Advanced System Design

The IH-5 utilizes the architecture of the ground-breaking IH-SR to set the standard for isolated heart perfusion in rabbits, adult guinea pigs, or adult rats. Engineered for the increased flow produced by these species (up to 500 mL/min), the IH-5 offers ultimate perfusion stability and real physiological conditions for longer, more relevant recordings with fewer artifacts. This compact platform has been optimized to create in situ-like perfusion features, delivering a considerably higher sensitivity for various experimental manipulation while maintaining the advantages of an ex vivo preparation. Like the IH-SR the IH-5 system does not utilize high water columns but creates a constant pressure perfusion setup using a pressure feedback pump controller, resulting in a compact system fully within reach of the researcher.  

The basic system is the retrograde perfusion mode (Langendorff). By setting a defined threshold pressure at the flow resistance, the system works under constant pressure; by increasing the threshold pressure to a supermaximal pressure (300 mmHg) the system works under constant flow. The complete system is mounted on a compact acrylic stand. 

The modular nature of the IH-5 allows the system to evolve along with your research. A Langendorff system can easily be upgraded to full Working Ejecting Heart (according to Neely), acommodating aortic flows up to 400 ml/min or to a biventricular system where both sides of the heart eject fluid as in vivo.

 Any IH-5 can also be upgraded with a range of measurement and application capabilities, such as multi-channel ECG and MAP cardiac electrophysiology applications. Other useful add-ons include drug addition (using a syringe pump) and pacing. If perfusate containing albumin or erythrocytes is used, the oxygenation can be optimized with the addition of a fiber oxygenator. The IH-5 utilizes convenient switches that allow the researcher to easily change from constant flow to constant pressure perfusion, Langendorff to Working Heart, and between two different perfusion solutions.

The IH-5 base system contains virually everything you need to accomplish the basic Langendorff experiment, only requiring the addition of the appropriate species-specific option, a heart chamber, the method of contant pressure perfusion, and the desired data acquisition method such as ISOHEART. Application-specific upgrades and options are listed below.

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Features & Benefits

  • Compact design, no high water columns; optimized individually for rabbit,rat or guinea pig
  • Constant pressure or constant flow perfusion in one unit, easily switch between the two modes
  • Suitable for hearts from hypertensive animals (perfusion pressures up to 300 mmHg are possible)
  • Fast and easy changes of perfusion pressure (within seconds)
  • Continuous measurement of heart mechanics (contractility), bioelectrical signals (ECG, MAP) and perfusate characteristics (pCO2, pO2, pH) 
  • Unique integrated small volume aortic block with built-in bubble trap and compliance chamber
  • Unique cannulae design
    • Cannula resistance is optimized according to Hagen-Poiseuilles Physical Law
    • Different cannula sizes available, all metal,no fragile glass cannulae
  • Proprietary mini holders allow easy and stress-free access to hold electrodes and catheters in position
  • Drug injection pathway built directly into aortic perfusate stream
  • Easily upgraded to a Working Heart (Ejecting Heart) System or biventricular working heart system; special flow resistance and compliance chamber closely mimics the in vivo afterload

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Applications

  • Study of myogene autoregulation with the addition of the Flow Measurement Option 
  • Testing with inotropic substances
  • Testing of lusitrope substances
  • Testing of vasoactive substances
  • Cardiac rhythm tests
  • Ischemia/hypoxia studies
  • Refractory period studies
  • Ischemia/reperfusion injury studies
  • Cardioplegia studies
  • Cardiac preconditioning
  • Cardiovascular screening performance
  • Electrophysiology studies
  • Phenotyping of transgenic animals 
  • Drug compound screening
  • Toxicology studies
  • Biochemical tests

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Measured Signals and Calculated Parameters

 The following signals are recorded as raw data in retrograde Langendorff perfusion:

  • Isovolumetric left ventricular pressure (balloon method)
  • Aortic (perfusion) pressure
  • Coronary flow*

The following parameters are calculated from the raw data (using the ISOHEART data acquisition software):

  • dLVP/dt, dLVP/dt Max, dLVP/dt Min, Contractility Index
  • Systolic and Diastolic LVP
  • Heart Rate
  • Mean Perfusion Pressure
  • Mean Perfusion Flow*
  • Coronary Resistance*

* This parameter is based on indirect flow measurement with SCP or direct ultrasound flow measurement with the TTFM-2 flowmeter and suitable flow probe.

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Included Items

IH-5 Core System, Basic, 230 V (73-4398) includes: IH-5 Core System, Basic, 115 V (73-4397) includes:
73-4996 IH-5 Base Unit* 73-4996 IH-5 Base Unit*
73-4544 Thermostatic Circulator TC120-ST5, Bath Volume 5 L, 230 V/50 Hz 73-4545 Thermostatic Circulator TC120-ST5, Bath Volume 5 L, 115 V/60 Hz
70-7001 Harvard Peristaltic Pump P230
70-7001 Harvard Peristaltic Pump P230
73-0045 PLUGSYS Case, Type 603 73-0045 PLUGSYS Case, Type 603
73-1793 PLUGSYS Transducer Amplifier Module (TAM-D) 73-1793 PLUGSYS Transducer Amplifier Module (TAM-D)

73-0065 PLUGSYS Transducer Amplifier Module (TAM-A) (Does not include balloon) 73-0065 PLUGSYS Transducer Amplifier Module (TAM-A) (Does not include balloon)

73-3862 APT300 Pressure Transducers (2 included) 73-3862 APT300 Pressure Transducers (2 included)
73-3871 Holder for APT300 Transducer for IH-5 73-3871 Holder for APT300 Transducer for IH-5

* The IH-5 Base Unit (73-4996) includes Plexiglas stand, aortic block with aorta link unit with Windkessel, heat exchanger, jacketed heart chamber, needle valve for adjusting gas flow, holder for transducer for perfusion pressure, tubings. Aortic cannula and buffer reservoir must be ordered separately.

For a functional unit, species-specific core options and pressure controller systems must be added.

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Additional Requirements for a Functional Langendorff Unit

An IH-SR Langendorff basic functional unit requires the addition of core options to the selected core system (either 73-4343 or 73-4344). Specifically, the core system requires the addition of a selection of one of the perfusion pressure controllers (SCP or STARLING-IHSR) and one of the species-specific options indicated in the table below:

 Langendorff Only Configuration
 (Add these core options to 73-4397 or 73-4398)
 Langendorff Working Heart-Ready Configuration
(Add these core options to 73-4397 or 73-4398)
 Species Specific Addition (choose one or both)
• 73-4399 for Rat/Guinea Pig Hearts (IH5-RGP)
• 73-4400 for Rabbit Hearts (IH5-RAB)

 Species Specific Addition (choose one or both)
• 73-4399 for Rat/Guinea Pig Hearts (IH5-RGP)
• 73-4400 for Rabbit Hearts (IH5-RAB)

 Constant Pressure/Flow Controller (required)
• 73-2806 SCP PLUGSYS Servo Controller Module 

 Perfusion Pressure Control (required)*
• 73-4435 STARLING-IH5 Starling Pressure Controller

Heart Chamber (choose one)
• 73-4401 for Cardiovascular Studies (IH5-HC-CARDIO)
• 73-4402 for Electrophysiology Studies (IH5-HC-EPHYS)**

Heart Chamber (choose one)
• 73-4401 for Cardiovascular Studies (IH5-HC-CARDIO)
• 73-4402 for Electrophysiology Studies (IH5-HC-EPHYS)**

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Additional Components

Please contact our technical team for help.

ISOHEART Data Acquisition Software

Stimulator and Electrodes for Pacing 

Aortic Cannulae

Buffer Reservoirs

Left Ventricular Pressure Kits (Balloons)

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Options & Upgrades

IH-5 Core System Options

 Options for required additions to the IH-5 Core System for species-specific Langendorff Only or Langendorff Working Heart-Ready configurations.

Working Heart Upgrade

The IH-5 Langendorff Core System is prepared for upgrade to a working ejecting heart  according to Neely later on. To upgrade an IH-5 Lagendorff Core System to a IH-5Working Heart System, add the Working Heart Upgrade and one of the species-specific additions. Researchers who know they will be upgrading to working ejecting heart in the future may start with a Langendorff Working Heart-Ready configuration. If you intend to measure coronary flow, you need a TTFM-2 flowmeter and a species suitable flowmeter. (SCP cannot be used for Working Heart.)

Langendorff Only configuration can easily be modified to a Langendorff Working Heart-Ready configuration. Please contact our technical team for help!

Coronary Flow Measurement Options for Working Heart

Flow measurement can be accomplished in an IH-SR unit using two methods: 

  • Indirect measurement by controlling the pump sped of a roller pump (SCP controller)
  • Direct measurement with Ultrasound Transit Time Technic (TTFM-2 module)

Oxygenating System Option (for foaming solutions)

Choose this option if you are using buffer supplemented with albumin, fatty acids, washed erythrocytes, or other foaming additives.

Cannulating Options (for metabolic studies)

Used for effluent collection from an IH-5 system for lfurther analysis, metabolic studies, or for continuous pO2, pH and pCO2 measurements.

LVP Measurement Option Working Heart

Easily allows introduction of a tip pressure catheter directly into the left ventricle via the adapter port and the aorta, rather than via apical puncture.

Pressure-Volume Loop Measurement Option for Working Heart

Easily allows introduction of a pressure-volume loop catheter directly into the left ventricle via an adapter port and the aorta.

Continuous Measurement of  pO2, pH and pCO2

The Universal Perfusion Solution Monitor permits precise continuous or discontinuous measurement in liquid media or perfusate of these three key parameters: pO2, pH and pCO2.

Single-Lead ECG and MAP Measurement

For single-lead ECG and MAP on functional IH-5 systems, one ECG lead is combined with one MAP electrode.

Multi-Lead ECG and MAP Measurement

For multi-lead ECG and MAP measurements on functional IH-5 Langendorff or working heart systems, up to 12 ECG leads are combined with up to 12 MAP electrodes.

Drug Addition

These options for drug addition can be added to any isolated organ system where flow is measured or calculated and a drug must be added in a certain ratio.

Temperature Measurement

Measure perfusate temperature in any isolated perfused organ system

Filtration of Recirculated Perfusate


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Specifications