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To study organs in isolation, certain requirements must be met. These include controlled temperature, a nutrient or buffer at physiological pH, aeration for the buffer, a perfusion pump to move the buffer and a system with a chamber to hold the isolated organ. Some organs may require additional equipment related to their specialized functions, such as a ventilator for isolated perfused lungs. Additionally, in order to ensure the health of the isolated organ, experimental conditions and various physiological parameters are monitored. These include perfusate flow and pressure, pH, pO2, pCO2, and biopotentials.

All functional isolated organ systems require the following components:

To ensure that your system is properly configured as a functional unit that meets your application needs, please complete our Checklist and then contact Technical Services at 800-547-6766 or or via email at  support@hbiosci.com before placing an order. In Europe, please call +49 7665 92000 or email at  sales@hugo-sachs.de.