EasyCell System for Cell Isolation
Used to isolate primary cells from hearts or other organs of species in the size range of mouse to guinea pigs.
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This perfusion system has been specifically created to isolate primary cells from hearts of species in the size range of mouse to guinea pigs. The system can also be configured for either in situ or ex vivo organ perfusion with the addition of an operating table or moist chamber and appropriate cannulae.
Primary Cell Isolation from Other Organs
Features & Benefits
- Simple constant flow perfusion system
- Easy to set up, operate and maintain
- Temperature control—water-jacketed heat exchanger and heart chamber for maintenance of physiological temperature
- Pulsation Dampening—air vessel/compliance chamber dampens perfusion pressure pulsation generated by the peristaltic pump. Serves as primary bubble trap to prevent air emboli from causing regional ischemia, increasing potential cell yield
- Wetted parts can be disinfected with 70% ethanol or autoclaved for sterilization
- Cardiomyocyte isolation from small rodent hearts
- Can be adapted for other small rodent organs in situ or ex vivo
- Basic research or student lab use
|230 V EasyCell System (73-4431)||115 V EasyCell System (73-5153)|
|73-3756||Easy Setup for Cell Extraction by Organ Disintegration, consisting of Base Stand with Clamps, Bubble Trap/Windkessel, Heating Coil, Protease Reservoir||73-3756||Easy Setup for Cell Extraction by Organ Disintegration consisting of Base Stand with Clamps, Bubble Trap/Windkessel, Heating Coil, Protease Reservoir|
|73-3761||Heart Chamber (ID 40 mm x 60 mm deep, Length 110 mm, OD 60 mm) with Mounting Clamp||73-3761||Heart Chamber (ID 40 mm x 60 mm deep, Length 110 mm, OD 60 mm) with Mounting Clamp|
|73-3438||Jacketed Reservoir for Buffer Solution with Frit, 1 L||73-3438||Jacketed Reservoir for Buffer Solution with Frit, 1 L|
|73-3456||Tube Set for Jacketed Buffer Reservoir with Fluid Line Shutoff Valve||73-3456||Tube Set for Jacketed Buffer Reservoir with Fluid Line Shutoff Valve|
|75-1004||Reglo Peristaltic (Roller) Pump||70-7000||Peristaltic Pump P70|
|73-4544||TC120 Thermocirculator, with 5 L stainless steel bath and lid, 220 V||73-4545||TC120 Thermocirculator, with 5 L stainless steel bath and lid, 120 V|
|73-1837||3-Stop Tygon® E-Lab Tubing, 2.29 mm ID, 12/pack, Purple/White||72-0668||3-Stop PVC Tubing, 2.05 mm ID, Purple/Purple, pkg. of 6|
|73-0126||3-Stop Tygon® E-Lab Tubing, 1.22 mm ID, 12/pack, Red/Grey||72-0662||3-Stop PVC Tubing, 1.14 mm ID, Red-Red, pkg. of 6|
|73-2798||Aortic Cannula with Luer Taper for Mice, OD 1.0 mm||73-2798||Aortic Cannula with Luer Taper for Mice, OD 1.0 mm|
|73-2868||Aortic Metal Cannula with Luer Taper for Small Rat, OD 2.0 mm||73-2868||Aortic Metal Cannula with Luer Taper for Small Rat, OD 2.0 mm|
|73-2871||Aortic Metal Cannula with Luer Taper for Rat/Guinea Pig, OD 3.0 mm||73-2871||Aortic Metal Cannula with Luer Taper for Rat/Guinea Pig, OD 3.0 mm|
Setup & Operation
The EasyCell System is a turnkey ex vivo heart perfusion system for cardiomyocyte isolation that is simple to setup, operate and maintain. Pressure monitoring can be added so that users can adjust flow rate to maintain a physiologic perfusion pressure.
Its compact design has a minimal footprint, conserving valuable bench space.
The heart is first perfused with a warmed, aerated standard perfusion buffer for blanching/clearing the heart of blood and then with a warmed, aerated protease solution for tissue disintegration. Unlike the PSCI system, the clearing buffer and collagenase share the same perfusion line, slightly delaying the delivery of the collagenase after switching over from the clearing buffer. The transition from clearing buffer to collagenase is accomplished by either moving the suction tube or turning a stopcock.
The EasyCell system has a single perfusion pathway that is fully compatible with ethanol for disinfection. The wetted components of the perfusion circuit can be flushed with a 70% ethanol solution or autoclaved for sterilization purposes should the cells need to be incubated for a number of hours following isolation rather than for immediate use.
Primary Cell Isolation from Other Organs
This perfusion system has been specifically created to isolate primary cells from organs of species in the size range of mouse to guinea pig. The system can be configured for either in situ or ex vivo organ perfusion.
Features & Benefits
- Simple to set up, operate and maintain
- Compact size minimizes bench space required
- Components of the perfusion circuit that come in contact with the perfusate can be flushed with 70% ethanol or autoclaved to sterilize
- Cell isolation from mouse, rat or guinea pig organs by disintegration using protease solutions
- Designed for cardiomyocyte isolation
- Can be used with other organs ex vivo or in situ
Setup and Operation
The system is first used with perfusate for the removal of red blood cells (blanching) and then with the protease solution for tissue disintegration. The perfusate for blanching is warmed and oxygenated in a separate buffer reservoir and supplied via a peristaltic pump and warming coil. The protease solution is in a separate reservoir, oxygenated, and stirred.
For in situ perfusion, an operating table can be placed just below the warming coil, for ex vivo applications (heart, liver, etc.) the system can be equipped with a jacketed chamber.
Harvard Apparatus offers a complete line of perfusion cannulae (aortic, ex vivo andin situ) to accommodate vessels from 1.0 to 3.5 mm ID. The ex vivo and in situ cannulae feature our unique tip basket to prevent vessel occlusion.
Base stand with clamps, bubble trap/Windkesselvessel, heating coil and protease reservoir
Additional equipment required
Thermocirculator, peristaltic pump, magnetic stirrer, reservoir, cannulae, operating table, transducer,monitoring system setup using the PLUGSYS Amplifier System