Equilibrium dialysis is a specific application of dialysis that is important for the study of the binding of small molecules and ions by proteins. It is one of several methods available for this purpose, and its attractive feature continues to be its physical simplicity. Another attractive feature of equilibrium dialysis is the ability to perform interaction studies without the use of fluorescent or radiolabeled tags.

Applications include: protein-drug binding assays, receptor binding assays, ligand binding assays, protein-protein interactions, protein-DNA interactions, serum protein binding

Overview of Equilibrium Dialysis