array(3) { ["show"]=> bool(true) ["ip"]=> string(13) "72.14.201.205" ["country"]=> string(2) "US" } desde la funcion de ip obtenemos IP invalida
harvardapparatus.com is a data controller for the purposes of the General Data Protection Regulation ("GDPR"). By proceeding to tick the consent box at right, you consent to harvardbioscience.com processing your personal data, under the GDPR and any other applicable legislation, that we collect from you in accordance with our Privacy Policy. If you wish to withdraw your consent for us to process your personal data please contact our Compliance Team. Please address any questions, comments and requests (including your right to withdraw your consent to process your personal data) regarding our data processing practices to our Compliance Team.
Allow

Overview of Equilibrium Dialysis

Equilibrium dialysis is a specific application of dialysis that is important for the study of the binding of small molecules and ions by proteins. It is one of several methods available for this purpose, and its attractive feature continues to be its physical simplicity. Another attractive feature of equilibrium dialysis is the ability to perform interaction studies without theuse of fluorescent or radiolabeled tags.

Generally, the objective of an equilibrium dialysis experiment is to measure the amount of a ligand bound to a macromolecule. This is typically done through an indirect process because in any mixture of the ligand and macromolecule, it is difficult to distinguish between the bound and free ligand. If, however, the free ligand can be dialyzed through a membrane, until its concentration across the membrane is at equilibrium, the free ligand concentration can be measured easily. Data obtained under different experimental conditions then provides information on various binding parameters of the compounds such as the binding constants and the number of binding sites or binding capacity.

Applications

  • Protein-drug binding assays
  • Receptor binding assays
  • Ligand binding assays
  • Protein-protein interactions
  • Protein-DNA interactions
  • Serum protein binding