IPL-4 Core Isolated Perfused Lung System for Rabbit to Fetal Pig

IPL-4 Core System

IPL-4 is an isolated organ perfusion system is specially designed for rabbit or fetal pig lung for measurement of key parameters associated with ex-vivo pulmonary assays.

Please note: For a functional unit, the core system requires the addition of a selection of a cannula set and core system options as well as a ventilator. Please see the Full Description for details.

To ensure that your system is properly configured as a functional unit that meets your application needs, please complete our Checklist and then contact Technical Services at 800-547-6766 or via email at support@hbiosci.com before placing an order. In Europe, please call +49 7665 92000 or via email at sales@hugo-sachs.de. Upgrades can also be modified to meet your specific needs.

Please view our NEW comprehensive catalog!

Item# Description U.S. List Price Quantity
73-4296 IPL-4 Core System for Isolated Rabbit to Fetal Pig Lung, 230 V
73-4297 IPL-4 Core System for Isolated Rabbit to Fetal Pig Lung, 115 V
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IPL-4 is an isolated organ perfusion system is specially designed for rabbit or fetal pig lung for measurement of key parameters associated with ex vivo pulmonary assays.

Advanced Engineering
Features & Benefits
Applications
Measured Signals & Calculated Parameters
Included items
Additional Requirements for a Functional Unit
Other Components
Options and Upgrades


 

 Advanced Engineering

The IPL-4 system is specially designed for rabbit or fetal pig lung. The unique in-situ cannulation system allows the researcher to align the cannulation head with the operating table while simultaneously retracting the lung chamber. No parts of the system need to be removed during cannulation. The basic system can easily be upgraded or customized to add capability modules for virtually any ex vivo pulmonary assay.

Ventilation

When the lung is placed in the artificial thoracic chamber and ventilated at negative pressure, the ventilation head is removed and replaced by the pneumotachometer for the measurement of respiratory flow. The ventilation medium (air or gas mixture) is constantly flowing past the distal end of the pneumotachometer directly via the tracheal cannula into the lungs. The breathing frequency can be varied between 30 and 60 breaths/min. In addition, the inspiration/expiration ratio as a percentage of each breath can be set between 10 and 90% in 10% steps. The end-inspiratory, end-expiratory, and deep breath (sigh or hyperinflation) negative pressure can be set individually. A deep breath (sigh) is usually induced every 5 to 20 min.

Constant Flow or Constant Pressure Perfusion

The lung can be perfused at constant flow or constant pressure. The SCP module regulates the pressure. Switching betwen the two modes is simply done by one switch on the SCP module. The lung is perfused via the pulmonary artery by a roller pump. The perfusate is stored in a water-jacketed container to maintain constant temperature. Aeration is provided to keep the pH constant. A heat exchanger and a bubble trap are placed next to the pulmonary artery connection for exact adjustment of the perfusate temperature just before the thorax chamber and to prevent air bubbles entering the lung.

Lung Mechanics

The pressure inside the thoracic chamber is measured with a MPX pressure transducer. Air flow is determined with the pneumotachometer connected to a DLP2.5 differential pressure transducer. The tidal volume is derived from the pulmonary air flow by integration. The calculation of pulmonary compliance and resistance needs a computer-controlled data acquisition system (HSE-HA PULMODYN). The IPL-4 core system contains virually everything you need to accomplish the basic perfusion and ventilation experiments, requiring only the addition of core options for ventillation. Application-specific upgrades and options are also available.

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Features & Benefits

  • Optimized temperature conditions for the isolated lung, unique jacketed thoracic chamber
  • Operating table for non-damaging in situ preparation
  • Negative-pressure ventilation similar to in vivo condition or positive-pressure ventilation available
  • Low flow resistance and dead space volume, minimize perfusion artifacts
  • Drug injection pathway built directly into pulmonary perfusate stream
  • Unique compensation system for vascular transmural pressure changes
  • More measurement parameters than any other system

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Applications

  • Investigation of ventilation and perfusion in the isolated rabbit or fetal pig lung
  • Optimized in-situ preparation
  • Negative-pressure ventilation to mimic closely the in-vivo situation
  • Regular induction of hyperinflation of the lung (sigh)
  • Perfusion under constant flow condition using buffer solution or blood
  • Continuous measurement of lung mechanics (tidal volume, resistance, compliance), and perfusate characteristics (pressure, pCO2, pO2, pH)
  • Continuous measurement of lung weight changes (edema)

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Measured Signals and Calculated Parameters

Signals recorded as raw data:

  • Pulmonary Artery (Perfusion) Pressure
  • Perfusion Flow
  • Intrapleural (artificial thorax) Pressure orTracheal Pressure
  • Respiratory Flow

Parameters calculated from raw data*:

  • Tidal Volume, Minute Volume
  • Peak Inspiratory and Expiratory Airflow
  • Vascular Resistance
  • Respiration Rate
  • Inspiratory Time & Expiratory Time
  • End Inspiratory and Expiratory Pressure
  • Dynamic Airway Resistance & Compliance

* Calculations are automatic when PULMODYN data acquisition software is used.

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Included Items

IPL-4 Core System,  230 V (73-4296) includes: IPL-4 Core System, 115 V (73-4297) includes:
Item # Description Item # Description
73-4138 Basic Unit for the Rabbit to Fetal Pig Isolated Perfused Lung*
73-4138 Basic Unit for the Rabbit to Fetal Pig Isolated Perfused Lung*
73-0125 Lauda Thermostatic Circulator, Type E-103, 230 V/50 Hz, 3 L Bath Volume, Temperature Range 20 to 150°C
73-2802 Lauda Thermostatic Circulator, Type E-103, 115 V/60 Hz, 3 L Bath Volume, Temperature Range 20 to 150°C
73-0322
Jacketed Glass Reservoir for Buffer Solution, with Frit, 6.0 L 73-0322
Jacketed Glass Reservoir for Buffer Solution, with Frit, 6.0 L
73-3456 Tube Set for Jacketed Buffer Reservoir with Fluid Line Shutoff Valves 73-3456 Tube Set for Jacketed Buffer Reservoir with Fluid Line Shutoff Valves
70-7001 Harvard Peristaltic Pump P-230 (Complete with Control Box and P-230 Motor Drive
70-7001 Harvard Peristaltic Pump P-230 (Complete with Control Box and P-230 Motor Drive

73-1863 2-Stop Tygon® E-Lab Tubing, 2.79 mm ID, 12/pack, Purple/White
73-1863
2-Stop Tygon® E-Lab Tubing, 2.79 mm ID, 12/pack, Purple/White
73-0045 PLUGSYS Case, Type 603 73-0045 PLUGSYS Case, Type 603
73-2806 Servo Controller for Perfusion (SCP) 73-2806 Servo Controller for Perfusion (SCP)
Perfusion Pressure Measurements
73-0020 Low Range Blood Pressure Transducer P75 for PLUGSYS Module 73-0020 Low Range Blood Pressure Transducer P75 for PLUGSYS Module
73-1793 PLUGSYS Transducer Amplifier Module (TAM-D) 73-1793 PLUGSYS Transducer Amplifier Module (TAM-D)
Respiratory Flow Measurements
59-9349 Heated Linear Pneumotachometer 0 to 10 L/min flow range 59-9349 Heated Linear Pneumotachometer 0 to 10 L/min flow range
59-9703 Heater Controller for Single Pneumotachometer 230 VAC, 50 Hz 59-9702 Heater Controller for Single Pneumotachometer 115 VAC, 60 Hz
73-3882
Differential Low Pressure Transducer DLP2.5, Range  +- 2.5 cmH2O, HSE Connector
73-3882 Differential Low Pressure Transducer DLP2.5, Range  +- 2.5 cmH2O, HSE Connector
73-0065 PLUGSYS Transducer Amplifier Module (TAM-A) 73-0065 PLUGSYS Transducer Amplifier Module (TAM-A)
Artificial Thorax Pressure
73-0064
Differential Pressure Transducer MPX, Range +- 100 cmH2O, HSE Connector
73-0064 Differential Pressure Transducer MPX, Range +- 100 cmH2O, HSE Connector
73-0065 PLUGSYS Transducer Amplifier Module (TAM-A)  73-0065 PLUGSYS Transducer Amplifier Module (TAM-A) 
73-2488  Operating Table, Size 5, for IPL-2* 73-2488  Operating Table, Size 5, for IPL-2*

*The basic unit includes:Plexiglass stand, jacketed lung chamber (artificial thorax), double Perspex disk that seals the chamber and contains the connectors that interface with the perfusion system, heat exchanger, humidifier and warm up system for breathing air, pressure equilibration vessel for the venous outflow, holders for the pressure transducers and all needed accessories (pulmonary artery cannula, left atrium cannula). Tracheal cannula, must be purchased separately (different diameters available).

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Additional Requirements for a Functional Unit

For a working unit, the core system requires the addition of a selection of either the VCMR-4 or PPV-4 in combination with the PPV-683 Small Animal Ventilator.

Negative Pressure Ventilation

  • VCM modules control the ventilation of the isolated lung preparation. They allow for the physiological negative pressure ventilation of the lung and positive ventilation during preparation.
    • 30 to 100 breaths per minute
    • I:E ratio can be set between 10 and 90% in 10% steps
    • End-inspiratory, end-expiratory, sigh and negative pressures can be individually set
  • The TCM Timer Counter Module allows periodic sigh (hyperinflation) breaths to minimize edema formation

Negative Pressure Ventilation Controller 
with Pressure Regulator for IPL-4*

VCMR-4 (73-4298) includes:
Utilizes 6 slot units

 73-1755 PLUGSYS VCM4-R Ventilation Control Module with Regulator 
 73-1750  PLUGSYS TCM Time Counter Module


* Negative pressure ventilation with a pressure regulator requires a pressurized gas supply from a tank or house air in the range of 2 to 8 bar (29 to 116 PSI) and the Pressure-Free Gas Supply Adapter (73-4312), purchased separately.

Positive Pressure Ventilation (PPV-4)

Choose the PPV-4 option when only positive pressure ventilation is desired. This adapter is used to connect to the small animal ventilator, Type 683. 

Positive Pressure Ventilation for IPL-4
includes:

 73-4312 Adapter for Positive Pressure Ventilation on IPL-4


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Other Components

Small Animal Ventilator, Type 683

The Model 683 Small Animal Ventilator comes standard with two piston and cylinder assemblies. For the IPL-4, the large piston must be installed. This allows tidal volumes to be set from 3.0 to 30 cc in 0.3 cc increments. By setting the cylinder stroke between gradations it is possible to achieve finer resolution in tidal volume settings. Respiratory rate can be set from 18 to 150 strokes per minute. Volume and rate settings can be adjusted while the ventilator is running.

 

Aerosol Nebulizer Kit (73-3433)

  • Low particle sizes (100% of the particles are below 10 µm)
  • No Solution Warming Required
  • Recommended for Nebulizing Drugs Sensitive to Ultrasonics
  • Aerosol is Automatically Transported by Compressed Air at a Pressure of 1.5 bar (22 PSI)
  • Aerosol nebulizer with multi-gas adapter kit (73-3433)
  • Pressure-free gas supply adapter for alternative gas supply for IPL-1 (73-2789)
  • Aerosol filter
  • Mounting hardware

PULMODYN Data Acquisition Software

Online evaluation of a wide range of signals and classical respiration parameters.

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 Options & Upgrades

Real-Time Flow Measurement

Direct real-time flow measurement with inline flow probe and TTFM-2 Transit Time Flowmeter Module

Continuous Measurement of  pO2, pH and pCO2

The Universal Perfusion Solution Monitor permits precise continuous or discontinuous measurement in liquid media or perfusate of these three key parameters: pO2, pH and pCO2.

Drug Addition

These options for drug addition can be added to any isolated organ system where flow is measured or calculated and a drug must be added in a certain ratio.

Temperature Measurement

Measure perfusate temperature in any isolated perfused organ system

Deoxygenation

For deoxygenation of blood or buffers containing proteins (e.g. albumin) or erythrocytes

Aerosol Nebulizer

Delivers an alternative gas supply to IPL-1 with option VCMP. Puchase with alternative gas supply PRGS-1

Filtration of Recirculated Perfusate

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Specifications
DOCUMENT DESCRIPTION
2018-HSE-HA-ISO-Lung.pdf2018 Isolated Lung Perfusion Systems Catalog/td>