pH Control Unit for IPL-2 (pHC-2)

pH Control Module for IPL-2

Controls and regulates the pH in the buffer solution by carefully bubbling with CO2. Used if Krebs-Henseleit (KH) buffer solution (KH) with albumin is used for perfusion.

KH stabilizes the pH at 7.4 by using 5% CO2. Carbogen has 95% O2 and 5% CO2 but 95% O2 are very high compared to normoxic situations. 

In order to keep the pH at 7.4 the perfusate is bubbled with CO2 on occasion. The pH is measured with a pH electrode, if the pH is higher than 7.4 a valve opens and the buffer is bubbled with CO2 until the pH is at 7.4. As soon as pH 7.4 is reached, bubbling stops.

Item# Description U.S. List Price Quantity
73-4282 Perfusate pH Control Option to IPL-2 Core System, 230 V (pHC-2)
73-4283 Perfusate pH Control Option to IPL-2 Core System, 115 V (pHC-2)
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This option controls and regulates the pH in the buffer solution by carefully bubbling with CO2.  Delivery of COmaintains pH when the system is not deoxygenated with an N2 /CO2 gas mixture. The pH is controlled by a feedback system which regulates CO2 gas bubbling into a dedicated small volume reservoir for minimizing foaming of perfusate.

Used if Krebs-Henseleit (KH) buffer solution (KH) with albumin is used for perfusion. KH stabilizes the pH at 7.4 by using 5% CO2. Carbogen has 95% O2 and 5% CO2 but 95% O2 are very high compared to normoxic situations. 

In order to keep the pH at 7.4 the perfusate is bubbled with CO2 on occasion. The pH is measured with a pH electrode, if the pH is higher than 7.4 a valve opens and the buffer is bubbled with CO2 until the pH is at 7.4. As soon as pH 7.4 is reached, bubbling stops.

Features & Benefits

  • pHCM Controller delivers gas (commonly CO2) by bubbling into mixed buffer to maintain a desired pH set point dictated by pHCM module
  • Minimizes foaming of perfusates due to minimal gassing of 100% gas to maintain pH  

Included Items

pH Control Unit for IPL-2, 230 V, pHC-2
(73-4282) includes:
pH Control Unit for IPL-2, 115 V, pHC-2
(73-4283) includes:
Item # Product Name Item # Product Name
73-2269  Jacketed Glass Container to Isolated Lung*  73-2269  Jacketed Glass Container to Isolated Lung*
73-0114 REGLO Analog 4 Channel MS-4/8-100, 230 VAC, 50 Hz  73-0113  REGLO Analog 4 Channel MS-4/8-100, 115 VAC, 60 Hz
73-0155 3-Stop Tygon® E-Lab Tubing, 2.79 mm ID, 12/pack, Purple/White  73-0155  3-Stop Tygon® E-Lab Tubing, 2.79 mm ID, 12/pack, Purple/White
69-0072 Foodtrode™ Protein Resistant pH Electrode  69-0072  Foodtrode™ Protein Resistant pH Electrode
69-0075 Protelyte™ Reference Electrolyte Solution, 100 ml  69-0075  Protelyte™ Reference Electrolyte Solution, 100 ml
69-0077 Electrode Connecting Cable, BNC to AS7, 1 m (3.3 ft)  69-0077  Electrode Connecting Cable, BNC to AS7, 1 m (3.3 ft)
73-1784 BNC-BNC Connecting Cable  73-1784  BNC-BNC Connecting Cable
73-0215 PLUGSYS pH Measurement Module (pHMM)  73-0215  PLUGSYS pH Measurement Module (pHMM)
73-1776 PLUGSYS pH Control Module (pHCM)  73-1776  PLUGSYS pH Control Module (pHCM)
73-2811 Mixer for IPL-2 Buffer Reservoir  73-2811  Mixer for IPL-2 Buffer Reservoir

Utilizes 4 slot units. Magnetic stirrer and stirbar sold separately

* With overflow to maintain constant perfusion solution level.

Specifications