Working Heart Upgrade for IH-SR Core System

The IH-SR Langendorff System can be upgraded to a fully-ejecting working heart system (according to Neely) for physiological cardiovascular studies, e.g., cardiac function and metabolism. The upgrade allows rapid and easy switching between working heart and Langendorff modes.

To upgrade our IH-SR Langendorff Core System to an IH-SR Working Heart System, add a Working Heart Upgrade and species-specific atrial cannulae.To measure flow, a TTFM-2 flowmeter and species-suitable flow probe are also required. Many other options are available for specialized applications.

Features & Benefits
Applications
Measured Signals & Calculated Parameters
Included Items
Species-Specific Additions
Specialized Applications & Upgrades

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Features & Benefits

• Optimized atrial cannulating conditions—true aortic flow and pressure signals (no bouncing afterload)
• Short atrial fill time (low flow resistance)—optimal ventricle filling
• Low resistance and dead space volume
• Minimal temperature and oxygen loss
• Unique vascular afterload system using a membrane—optimal vascular system including compliance simulation
• Physiological atrial and arterial pressure waveform—avoids non-physiological pressure wave; no dynamic pressure effects or altered coronary flow
• Read constant preload system—preload pressure independent of the atrial flow; no stress on mitral valve
• Low aortic cannula flow resistance—possible to insert a Millar transducer for LVP or LVP/Volume measurement
• No liquid pressure column as afterload—less stress on valves
• All electrodes, catheters and probes are fully enclosed  in the unique closed jacketed chamber—easy, direct access to the heart and to maintain physiological conditions while performing the experiments.

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Applications

• Real-time flow (cardiac output) measurement (add species-appropriate flow probes and PLUGSYS TTFM-2 Flowmeter module)
• Left ventricular pressure (LVP) measurement (add LVP Measurement option)
• Pressure-volume measurement (add Pressure-Volume Measurement option)
• High blood pressure-induced disease state simulation (add Increased Preload Pressure option)

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Measured Signals & Calculated Parameters

All parameters of standard Langendorff plus the following signals can be recorded:

• Preload (left atrial preload/ventricular filling pressure)
• Afterload (determining the diastolic and systolic aortic pressure)
• Intracardial left ventricular pressure or pressure-volume loops
• Atrial, aortic and coronary flow (calculated from atrial and aortic flow)

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Included Items

The Working Heart Option (73-2818) includes:

Left atrium cannulating system consisting of preload reservoir (capacity 2 to 7 ml), movable atrium connection adapter, and holder for P75 pressure transducer for preload measurement. Atrial cannulae must be ordered separately.

Atrial Cannulae (Required, Purchase Separately)

Complete the Working Heart upgrade by choosing the appropriate species-specific addition:

• 73-4033 Working Heart Left Atrial Cannula for Mouse Heart, 1.3 mm OD
• 73-4034 Working Heart Left Atrial Cannula for Rat and Guinea Pig Heart, 2.3 mm OD

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Specialized Applications & Upgrades

Left Atrial and Aortic Flow Measurement Option 

Flow measurement can be accomplished in an IH-SR unit using two methods: 

• Indirect measurement by controlling the pump sped of a roller pump (SCP controller)
• Direct measurement with Ultrasound Transit Time Technic (TTFM-2 module)

Intracardial Left Ventricular Pressure (LVP) Measurement Option

Easily allows introduction of a tip pressure catheter directly into the left ventricle via the adapter port and the aorta, rather than via apical puncture.

Pressure-Volume Loop (PVL) Measurement Option

Easily allows introduction of a pressure-volume loop catheter directly into the left ventricle via an adapter port and the aorta.

Increased Preload Pressure Option

Choose this option when you need to create left atrial preload pressures higher than 11 mmHg. (Gottlieb Valve).

Coronary Effluent Collection

Pulmonary artery cannulae, preparation dish and effluent funnel for collectionof effluent from the coronaries for metabolic studies, further analysis, or for continuous pO2,pH, or pCO2 measurements. 

Perfusate Oxygenation Option

For oxygenation of buffer solution supplemented with albumin, fatty acids, washed erythrocytes, or other foaming additives.

Perfusate Filtration Option

For filtration of recirculated perfusate.

Temperature Measurement Option

Measure perfusate temperature in any isolated perfused organ system. Since the system is well thermostated, temperature measurement is only necessary when specific temperature studies with special temperature protocols are being performed, e.g. cooling or transplantation studies.

Perfusion Solution Monitoring

Permits precise continuous or intermittent measurement in liquid media or perfusate of three key parameters: pO₂, pH and pCO₂.

Drug Addition Option

These options for drug addition can be added to any isolated organ system where flow is measured or calculated and a drug must be added in a certain ratio.

Cell Isolation Upgrade for IH-SR

Add this upgrade to a working IH-SR system for cell isolation/cell extraction applications.

Pacing Option

For addition of pacing to functional IH-SR or IH-5 Langendorff and Working HeartSystems. For small rodent hearts (mouse, rat and guinea pig). 

Single-Lead ECG and MAP Measurement Options

For single-lead ECG and MAP on functional IH-5 systems, one ECG lead is combined with one MAP electrode.

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