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Gel Filtration Chromatography SpinColumns (Size Exclusion)

Price on Request
SKU
GelF-GRP

QuikPrep® SpinColumns™ for gel fitration chromatography can be used for protein purification, buffer exchange, desalting, or for group separation.

Gel filtration, also called size-exclusion chromatography is an easy-to-use method for separation of molecules with different molecular sizes, using mild conditions.

Grouped product items
Item No. Description Qty
74-7220 Ultra-Micro SpinColumns, G-10 , Qty. of 24 Please login to add to Quote
74-7200 Ultra-Micro SpinColumns, G-10, Qty. of 96 Please login to add to Quote
74-4504 Micro SpinColumns, G-10, Qty. of 24 Please login to add to Quote
74-4500 Micro SpinColumns, G-10, Qty. of 96 Please login to add to Quote
74-3904 Macro SpinColumns, G-10, Qty. of 24 Please login to add to Quote
74-3900 Macro SpinColumns, G-10, Qty. of 96 Please login to add to Quote
74-5611 96-Well Micro SpinColumns, G-10, 1.1 ml Reservoir Plate (1) Please login to add to Quote
74-5651 96-Well Macro SpinColumns, G-10, 1.1 ml Reservoir Plate (1) Please login to add to Quote
74-7221 Ultra-Micro SpinColumns, G-25, Qty. of 24 Please login to add to Quote
74-7201 Ultra-Micro SpinColumns, G-25, Qty. of 96 Please login to add to Quote
74-4505 Micro SpinColumns, G-25, Qty. of 24 Please login to add to Quote
74-4501 Micro SpinColumns, G-25, Qty. of 96 Please login to add to Quote
74-3905 Macro SpinColumns, G-25, Qty. of 24 Please login to add to Quote
74-3901 Macro SpinColumns, G-25, Qty. of 96 Please login to add to Quote
74-5612 96-Well Micro SpinColumns, G-25, 1.1 ml Reservoir Plate (1) Please login to add to Quote
74-5652 96-Well Macro SpinColumns, G-25, 1.1 ml Reservoir Plate (1) Please login to add to Quote
74-7222 Ultra-Micro SpinColumns, G-50, Qty. of 24 Please login to add to Quote
74-7202 Ultra-Micro SpinColumns, G-50, Qty. of 96 Please login to add to Quote
74-4506 Micro SpinColumns, G-50, Qty. of 24 Please login to add to Quote
74-4502 Micro SpinColumns, G-50, Qty. of 96 Please login to add to Quote
74-3906 Macro SpinColumns, G-50, Qty. of 24 Please login to add to Quote
74-3902 Macro SpinColumns, G-50, Qty. of 96 Please login to add to Quote
74-5613 96-Well Micro SpinColumns, G-50, 1.1 ml Reservoir Plate (1) Please login to add to Quote
74-5653 96-Well Macro SpinColumns, G-50, 1.1 ml Reservoir Plate (1) Please login to add to Quote
74-7223 Ultra-Micro SpinColumns, G-100, Qty. of 24 Please login to add to Quote
74-7203 Ultra-Micro SpinColumns, G-100, Qty. of 96 Please login to add to Quote
74-4507 Micro SpinColumns, G-100, Qty. of 24 Please login to add to Quote
74-4503 Micro SpinColumns, G-100, Qty. of 96 Please login to add to Quote
74-3907 Macro SpinColumns, G-100, Qty. of 24 Please login to add to Quote
74-3903 Macro SpinColumns, G-100, Qty. of 96 Please login to add to Quote
74-5614 96-Well Micro SpinColumns, G-100, 1.1 ml Reservoir Plate (1) Please login to add to Quote
74-5654 96-Well Macro SpinColumns, G-100, 1.1 ml Reservoir Plate (1) Please login to add to Quote
74-7224 Ultra-Micro SpinColumns, P-2, Qty. of 24 Please login to add to Quote
74-7204 Ultra-Micro SpinColumns, P-2, Qty. of 96 Please login to add to Quote
74-4808 Micro SpinColumns, P-2, Qty. of 24 Please login to add to Quote
74-4802 Micro SpinColumns, P-2, Qty. of 96 Please login to add to Quote
74-4308 Macro SpinColumns, P-2, Qty. of 24 Please login to add to Quote
74-4302 Macro SpinColumns, P-2, Qty. of 96 Please login to add to Quote
74-5615 96-Well Micro SpinColumns, P-2, 1.1 ml Reservoir Plate (1) Please login to add to Quote
74-5655 96-Well Macro SpinColumns, P-2, 1.1 ml Reservoir Plate (1) Please login to add to Quote
74-7225 Ultra-Micro SpinColumns, P-6, Qty. of 24 Please login to add to Quote
74-7205 Ultra-Micro SpinColumns, P-6, Qty. of 96 Please login to add to Quote
74-4809 Micro SpinColumns, P-6, Qty. of 24 Please login to add to Quote
74-4803 Micro SpinColumns, P-6, Qty. of 96 Please login to add to Quote
74-4309 Macro SpinColumns, P-6, Qty. of 24 Please login to add to Quote
74-4303 Macro SpinColumns, P-6, Qty. of 96 Please login to add to Quote
74-5616 96-Well Micro SpinColumns, P-6, 1.1 ml Reservoir Plate (1) Please login to add to Quote
74-5656 96-Well Macro SpinColumns, P-6, 1.1 ml Reservoir Plate (1) Please login to add to Quote
74-3908 Macro SpinColumn, P-30, Qty. of 24 Please login to add to Quote
74-3892 Macro SpinColumn, P-30, Qty. of 96 Please login to add to Quote

QuikPrep® SpinColumns™ for gel filtration chromatography can be used for protein purification, buffer exchange, desalting, or for group separation.

Gel filtration, also called size-exclusion chromatography is an easy-to-use method for separation of molecules with different molecular sizes, using mild conditions. Gel Filtration uses the size of molecules in solution to determine separation.

SpinColumns have short media packing so the samples are separated by size, the large molecules travel out of the column with the void volume the smaller molecules, salts, etc. remain in the column. Desalting columns are used not only to remove low molecular weight contaminants such as salt, but also for buffer exchange before and after different chromatography techniques and for the rapid removal of reagents to terminate a reaction.

Examples of group separations include:

  • Removal of unincorporated nucleotides during DNA sequencing
  • Removal of free low molecular weight labels
  • Termination of reactions between macromolecules and low molecular weight reactants
  • Removal of products, cofactors or inhibitors from enzymes
  • Removal of unreacted radiolabels such as [α-32P]-ATP from nucleic acid labeling reactions

Available Matrices (Packing Materials)

Matrix Particle Diameter, µm Fractionation Range, Da Exclusion Limit, Da Applications
G-10 40 to 120 (dry) <700  700 Desalting peptides
G-25 40 to 120 (dry) 1,000 to 5,000  5,000 Desalting proteins and nucleic acids
G-50 20 to 80 (dry) 1,000 to 30,000  30,000 Removal of free labels from labeled macromolecules
G-100 40 to 120 (dry) 4,000 to 150,000  150,000 Molecular weight determination
P-2 <45 (wet)  100 to 1,800  1,800 Rapid carbohydrate and small peptide separations and desalting
P-6 90 to 180 (wet) 
1,000 to 8,000  6,000 Purification of proteins and polypeptides
P-30 90 to 180 (wet) 
2,500 to 40,000  40,000 Purification of proteins

G-10, G-25, G-50- G-100 = Sephadex    P-2, P-6, P-30 = Porous polyacrylamide beads

For additional column specifications and technical information, please download our Guide to Gel Fitration or Size Exclusion Chromatography.

 

 

Available Matrices (Packing Materials)

Matrix Particle Diameter, µm Fractionation Range, Da Exclusion Limit, Da Applications
G-10 40 to 120 (dry) <700  700 Desalting peptides
G-25 40 to 120 (dry) 1,000 to 5,000  5,000 Desalting proteins and nucleic acids
G-50 20 to 80 (dry) 1,000 to 30,000  30,000 Removal of free labels from labeled macromolecules
G-100 40 to 120 (dry) 4,000 to 150,000  150,000 Molecular weight determination
P-2 <45 (wet)  100 to 1,800  1,800 Rapid carbohydrate and small peptide separations and desalting
P-6 90 to 180 (wet) 
1,000 to 8,000  6,000 Purification of proteins and polypeptides
P-30 90 to 180 (wet) 
2,500 to 40,000  40,000 Purification of proteins

G-10, G-25, G-50- G-100 = Sephadex    P-2, P-6, P-30 = Porous polyacrylamide beads

SpinColumn Types

Column Type Sample Volume Sample Capacity Suggested
Elution Volume
Included Choice of Packing Materials
(Matrices)
Ultra-Micro  10 µl to 25 µl 3 to 30 µg
 28.5 µl

Two 2 ml centrifuge tubes with top caps

G-10, G-25, G-50, G-100, P-2, P-6
Micro  25 µl to 75 µl 5 to 60 µg
 50 µl
Two 2 ml centrifuge tubes with top caps G-10, G-25, G-50, G-100, P-2, P-6
Macro  75 µl to 150 µl 30 to 300 µg
 143 µl
Two 2 ml centrifuge tubes with top and bottom caps G-10, G-25, G-50, G-100, P-2, P-6, P-30

96-Well Micro  25 µl to 75 µl 5 to
60 µg
 50 µl
Two 96-well collection plates (1.1 ml per well) G-10, G-25, G-50, G-100, P-2, P-6

96-Well Macro  25 µl to
150 µl
30 to
300 µg
 143 µl
Two 96-well collection plates (1.1 ml per well) G-10, G-25, G-50, G-100, P-2, P-6

For additional column specifications and technical information, please download our Guide to Gel Fitration or Size Exclusion Chromatography.