Anion Exchange Chromatography SpinColumns
QuikPrep® SpinColumns™ for anion exchange chromatography are filled with a positively charged resin to attract negatively charged molecules from samples.
Matrices are available in either strong or weak ionized states. This expresses the ability of the exchange matrix to maintain its charge with a change in pH and not the strength of the bond. Strong exchangers have functional groups that will always remain ionized, while the functional groups of the weak exchangers can be neutralized by changing the pH.
QuikPrep® SpinColumns™ for anion exchange chromatography are filled with a positively charged resin to attract negatively charged molecules from samples.
Matrices are available in either strong or weak ionized states. This expresses the ability of the exchange matrix to maintain its charge with a change in pH and not the strength of the bond. Strong exchangers have functional groups that will always remain ionized, while the functional groups of the weak exchangers can be neutralized by changing the pH.
Available Matrices (Packing Materials)
| Matrix | Functional Group | pH Range | Ionic Capacity | Applications |
| Strong Anion Q |
Quaternary ammonium | 2 to 12 | 0.18 to 0.25 mmol (sulfate ion)/ml | High molecular weight protein separation |
| Weak Anion DEAE |
Diethylaminoethyl | 5 to 9 | 0.11 to 0.16 mmol (Cl-)/ml | Protein separation |
| Weak Anion PEI |
Linear polyethylenimine | 4 to 8 | 0.4 to 0.5 mmol (OH-)/g | Peptide, protein, nucleic acid and oligonucleotide separation |
Q = Q-Sepharose Fast Flow (quaternary amine sepharose) DEAE = Diethylaminoethlyl-cellulose
Linear PEI = PolyWAX LP™ (silica based)
About Ion Exchange Chromatography
Ion exchange chromatography (IEX) is an effective method of sample purification and fractionation based on molecular charge. Samples are passed through spin columns containing a predetermined matrix. Target molecules are captured and held by ionic bonds to the insoluble stationary matrix while the remaining sample is allowed to flow through the column. The ionic bonds are then broken through the addition of an elution buffer which changes the pH and alters the ionic strength of the molecules being retained. The bound molecules are eluted in order of the least to most strongly bound allowing for the collection of samples in fractions for separate analysis.
Available Matrices (Packing Materials)
| Matrix | Functional Group | pH Range | Ionic Capacity | Applications |
| Strong Anion Q |
Quaternary ammonium | 2 to 12 | 0.18 to 0.25 mmol (sulfate ion)/ml | High molecular weight protein separation |
| Weak Anion DEAE | Diethylaminoethyl | 5 to 9 | 0.11 to 0.16 mmol (Cl-)/ml | Protein separation |
| Weak Anion PEI | Linear polyethylenimine | 4 to 8 | 0.4 to 0.5 mmol (OH-)/g | Peptide, protein, nucleic acid and oligonucleotide separation |
Q = Q-Sepharose Fast Flow (quaternary amine sepharose) DEAE = Diethylaminoethlyl-cellulose Linear PEI = PolyWAX LP™ (silica based)
SpinColumn Types
| Column Type | Sample Volume | Sample Capacity | Suggested Elution Volume |
Included | Choice of Packing Materials (Matrices) |
| Ultra-Micro | 10 µl to 25 µl | 3 to 30 µg |
28.5 µl |
Two 2 ml centrifuge tubes with top caps |
Strong Anion Q, Weak Anion DEAE, Weak Anion PEI |
| Micro | 25 µl to 75 µl | 5 to 60 µg |
50 µl |
Two 2 ml centrifuge tubes with top caps | Strong Anion Q, Weak Anion DEAE, Weak Anion PEI |
| Macro | 75 µl to 150 µl | 30 to 300 µg |
143 µl |
Two 2 ml centrifuge tubes with top and bottom caps | Strong Anion Q, Weak Anion DEAE, Weak Anion PEI |
| 96-Well Micro | 25 µl to 75 µl | 5 to 60 µg |
50 µl |
Two 96-well collection plates (1.1 ml per well) | Strong Anion Q, Weak Anion DEAE, Weak Anion PEI |
| 96-Well Macro | 25 µl to 150 µl |
30 to 300 µg |
143 µl |
Two 96-well collection plates (1.1 ml per well) | Strong Anion Q, Weak Anion DEAE, Weak Anion PEI |
