Abdominal Organ Chambers for Large Animal Liver or Kidney

Flexible and useful standard jacketed and fully heated chamber for ex-vivio perfusion of most abdominal organs from large animal models. For perfusion studies with pig liver or pig kidney, such as transplantation studies, xenotransplantation studies, pharmacological research with blood or erythrocyte-containing perfusate.

This chamber has been designed to perfuse the above mentioned isolated organs under best physiological temperatured conditions. Organ perfusion of a few hours to one day are possible. 

Features & Benefits
Applications
Application Examples
Included and Required Components

Features & Benefits

• Jacketed heated chamber maintains physiological temperature conditions
• Allows for constant flow or pressure perfusion in a single system
• Ability to expand set up to monitor and record pressure, flow, pH, pO₂, pCO₂ and temperature
• Compatible with a variety of accessories, making it suitable for a wide range of applications
• Provides a complete perfusion system when combined with a perfusion control system with pressure and flow regulation

Applications

• For use in physiological or pharmacological researchfor the perfusion of a pig liver or kidney with blood or erythrocyte-containing perfusate
• For liver or kidney transplantation studies
• For liver or kidney xenotransplantation studies

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Application Examples

Plg Liver

The pig liver to be perfused is placed in a moist, thermostated chamber (inside dimensions: 400 x 300 x 180 mm) and perfused with blood or erythrocyte-containing perfusate under constant-flow conditions via the portal vein. A centrifugal pump with a gentle action on blood is used to reduce hemolysis. Since this type of pump does not supply a constant flow or pressure, the constant flow is maintained by an electronic controller (SCP).

Pig Kidney

For the kidney, instead of the liver chamber, a smaller chamber (inside dimensions 260 x 200 x 210 mm) is used. The kidney is mainly perfused at constant pressure, which is also controlled by an SCP controller module.

Application Example

The pig liver (or pig kidney) to be perfused is placed in the moistened, thermostated chamber and perfused with blood through the portal vein under
constant-flow conditions. (In case of a pig kidney the renal artery and renal vein are used to cannulate and constant pressure perfusion has to be used.)

A centrifugal pump with a gentle action on blood is employed and required to reduce hemolysis. It is controlled by an electronic controller (SCP) so that the apparatus can be operated under constant-flow conditions or under constant-pressure conditions. Flow control requires that the flow is measured by means of a flow probe and a flowmeter (TTFM-2).

The perfusion pressure should also be measured to monitor the experiment. This requires a pressure transducer (P75 or APT300) and a bridge amplifier (TAM).

A suitable oxygenator is necessary for oxygenating and warming the blood. It can be of a type used in human medicine, e.g. METRONIC, CARMEDIA, AFFINITY NT or similar. Due to law regulations we are not allowed to sell such types of oxygenators, they must be purchsed by the user.

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Included and Required Components

Included items: Jacketed plexiglass chamber with connections for perfusion tubes, jacketed cover, tubing for thermostatic circulation, tubing for perfusion

Additional equipment required: Thermocirculator, pump with analog control*, Servo Controlled Perfusion System (SCP), measurement system for flow and pressure, oxygenator with heat exchanger, e.g. Terumo, Capiox SX10® or Medtronic MinimaxPlus PRF®, monitoring system setup using the PLUGSYS Amplifier System. Recording and evaluation of the signals uses BDAS orLabChart software.

* For blood we recommend a Pump Drive BVP-ZX with centrifugal pump head.

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