Gel Filtration SpinColumns

Gel Filtration Spin Columns

Gel Filtration SpinColumns can be used for protein purification, buffer exchange, desalting, or for group separation.

Gel filtration, also called size-exclusion chromatography is an easy-to-use method for separation of molecules with different molecular sizes, using mild conditions.

Item# Description U.S. List Price Quantity
74-7220 Ultra-Micro Spin Columns, G-10 , Qty. of 24
74-7200 Ultra-Micro Spin Columns, G-10, Qty. of 96
74-7221 Ultra-Micro Spin Columns, G-25, Qty. of 24
74-7201 Ultra-Micro Spin Columns, G-25, Qty. of 96
74-7222 Ultra-Micro Spin Columns, G-50, Qty. of 24
74-7202 Ultra-Micro Spin Columns, G-50, Qty. of 96
74-7223 Ultra-Micro Spin Columns, G-100, Qty. of 24
74-7203 Ultra-Micro Spin Columns, G-100, Qty. of 96
74-7224 Ultra-Micro Spin Columns, P-2, Qty. of 24
74-7204 Ultra-Micro Spin Columns, P-2, Qty. of 96
74-7225 Ultra-Micro Spin Columns, P-6, Qty. of 24
74-7205 Ultra-Micro Spin Columns, P-6, Qty. of 96
74-4504 Micro Spin Columns, G-10, Qty. of 24
74-4500 Micro Spin Columns, G-10, Qty. of 96
74-4505 Micro Spin Columns, G-25, Qty. of 24
74-4501 Micro Spin Columns, G-25, Qty. of 96
74-4506 Micro Spin Columns, G-50, Qty. of 24
74-4809 Micro Spin Columns, P-6, Qty. of 24
74-4803 Micro Spin Columns, P-6, Qty. of 96
74-4502 Micro Spin Columns, G-50, Qty. of 96
74-4507 Micro Spin Columns, G-100, Qty. of 24
74-4503 Micro Spin Columns, G-100, Qty. of 96
74-4808 Micro Spin Columns, P-2, Qty. of 24
74-4802 Micro Spin Columns, P-2, Qty. of 96
74-3904 Macro Spin Columns, G-10, Qty. of 24
74-3900 Macro Spin Columns, G-10, Qty. of 96
74-3905 Macro Spin Columns, G-25, Qty. of 24
74-3901 Macro Spin Columns, G-25, Qty. of 96
74-3906 Macro Spin Columns, G-50, Qty. of 24
74-3902 Macro Spin Columns, G-50, Qty. of 96
74-3907 Macro Spin Columns, G-100, Qty. of 24
74-3903 Macro Spin Columns, G-100, Qty. of 96
74-4308 Macro Spin Columns, P-2, Qty. of 24
74-4302 Macro Spin Columns, P-2, Qty. of 96
74-4309 Macro Spin Columns, P-6, Qty. of 24
74-4303 Macro Spin Columns, P-6, Qty. of 96
74-3908 Macro SpinColumn, P-30, Qty. of 24
74-3892 Macro SpinColumn, P-30, Pkg. 96
74-5611 96-Well Micro Spin Columns, G-10, 1 Plate
74-5612 96-Well Micro Spin Columns, G-25, 1 Plate
74-5613 96-Well Micro Spin Columns, G-50, 1 Plate
74-5614 96-Well Micro Spin Columns, G-100, 1 Plate
74-5615 96-Well Micro Spin Columns, P-2,1 Plate
74-5616 96-Well Micro Spin Columns, P-6,1 Plate
74-5651 96-Well Macro Spin Columns, G-10, 1 Plate
74-5652 96-Well Macro Spin Columns, G-25, 1 Plate
74-5653 96-Well Macro Spin Columns, G-50, 1 Plate
74-5654 96-Well Macro Spin Columns, G-100, 1 Plate
74-5655 96-Well Macro Spin Columns, P-2,1 Plate
74-5656 96-Well Macro SpinColumns, P-6, 1 Plate
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Gel Filtration SpinColumns can be used for protein purification, buffer exchange, desalting, or for group separation.

Gel filtration, also called size-exclusion chromatography is an easy-to-use method for separation of molecules with different molecular sizes, using mild conditions. Gel Filtration uses the size of molecules in solution to determine separation.

SpinColumns have short media packing so the samples are separated by size, the large molecules travel out of the column with the void volume the smaller molecules, salts, etc. remain in the column. Desalting columns are used not only to remove low molecular weight contaminants such as salt, but also for buffer exchange before and after different chromatography techniques and for the rapid removal of reagents to terminate a reaction.

Examples of group separations include:

  • Removal of unincorporated nucleotides during DNA sequencing
  • Removal of free low molecular weight labels
  • Termination of reactions between macromolecules and low molecular weight reactants
  • Removal of products, cofactors or inhibitors from enzymes
  • Removal of unreacted radiolabels such as [α-32P]-ATP from nucleic acid labeling reactions

Available Matrices (Packing Materials)

Matrix Dia µm MWCO, Da Applications
 G-10*  40-120  ≤ 700  Desalting peptides
 G-25*  40-120  ≤ 1,500  Desalting proteins and nucleic acids
 G-50*  50-150  100-5,000  Removal of free labels from labeled macromolecules
G-100*  40-150 1,000-10,000   Molecular weight determination 
 P-2**  45-90 100-1,800  Rapid carbohydrate and small peptide separations and desalting
 P-6**  45-90 1,000-6,000  Purification of proteins and polypeptides
P-30**  45-90  2,500-40,000  Purification of proteins

*Sephadex  **Polyacrylamide

Column Types

Column Type Sample Volume Bed Volume Sample Concentration Included Choice of Packing Materials (Matrices)
Ultra-Micro  10 µl - 25 µl 37.96 µl 3 - 30 µg

Two 2 ml centrifuge tubes with top caps

G-10, G-25, G-50, G-100, P-2, P-6
Micro  25 µl - 75 µl 66.42 µl
5 - 60 µg
Two 2 ml centrifuge tubes with top caps G-10, G-25, G-50, G-100, P-2, P-6
Macro  75 µl - 150 µl 191.45 µl
30- 300 µg
Two 2 ml centrifuge tubes with top and bottom caps G-10, G-25, G-50, G-100, P-2, P-6, P-30
96-Well Micro  25 µl - 75 µl 66.42 µl
5 - 60 µg
Two 96-well collection plates (1.1 ml per well)* G-10, G-25, G-50, G-100, P-2, P-6
96-Well Macro  25 µl - 150 µl 191.45 µl
30- 300 µg
Two 96-well collection plates (1.1 ml per well)* G-10, G-25, G-50, G-100, P-2, P-6

* Addtional 96-well spin columns with 450 µl per well collection plates are available.

For additional column specifications and technical information, please download our Guide to Gel Fitration or Size Exclusion Chromatography.

 

 

Specifications
Column Type Sample Volume Bed Volume Sample Concentration Included Choice of Packing Materials (Matrices)
Ultra-Micro  10 µl - 25 µl 37.96 µl 3 - 30 µg

Two 2 ml centrifuge tubes with top caps

G-10, G-25, G-50, G-100, P-2, P-6
Micro  25 µl - 75 µl 66.42 µl
5 - 60 µg
Two 2 ml centrifuge tubes with top caps G-10, G-25, G-50, G-100, P-2, P-6
Macro  75 µl - 150 µl 191.45 µl
30- 300 µg
Two 2 ml centrifuge tubes with top and bottom caps G-10, G-25, G-50, G-100, P-2, P-6, P-30
96-Well Micro  25 µl - 75 µl 66.42 µl
5 - 60 µg
Two 96-well collection plates (1.1 ml per well)* G-10, G-25, G-50, G-100, P-2, P-6
96-Well Macro  25 µl - 150 µl 191.45 µl
30- 300 µg
Two 96-well collection plates (1.1 ml per well)* G-10, G-25, G-50, G-100, P-2, P-6

* Addtional 96-well spin columns with 450 µl per well collection plates are available.

For additional column specifications and technical information, please download our Guide to Gel Fitration or Size Exclusion Chromatography.