Gel Filtration SpinColumns (Size Exclusion)

Gel Filtration SpinColumns (Size Exclusion)

QuikPrep SpinColumns for gel fitration chromatography can be used for protein purification, buffer exchange, desalting, or for group separation.

Gel filtration, also called size-exclusion chromatography is an easy-to-use method for separation of molecules with different molecular sizes, using mild conditions.

Item# Description U.S. List Price Quantity
74-7220 Ultra-Micro SpinColumns, G-10 , Qty. of 24
74-7200 Ultra-Micro SpinColumns, G-10, Qty. of 96
74-4504 Micro SpinColumns, G-10, Qty. of 24
74-4500 Micro SpinColumns, G-10, Qty. of 96
74-3904 Macro SpinColumns, G-10, Qty. of 24
74-3900 Macro SpinColumns, G-10, Qty. of 96
74-5611 96-Well Micro SpinColumns, G-10, 1.1 ml Reservoir Plate (1)
74-5651 96-Well Macro SpinColumns, G-10, 1.1 ml Reservoir Plate (!)
74-7221 Ultra-Micro SpinColumns, G-25, Qty. of 24
74-7201 Ultra-Micro SpinColumns, G-25, Qty. of 96
74-4505 Micro SpinColumns, G-25, Qty. of 24
74-4501 Micro SpinColumns, G-25, Qty. of 96
74-3905 Macro SpinColumns, G-25, Qty. of 24
74-3901 Macro SpinColumns, G-25, Qty. of 96
74-5612 96-Well Micro SpinColumns, G-25, 1.1 ml Reservoir Plate (1)
74-5652 96-Well Macro SpinColumns, G-25, 1.1 ml Reservoir Plate (1)
74-7222 Ultra-Micro SpinColumns, G-50, Qty. of 24
74-7202 Ultra-Micro SpinColumns, G-50, Qty. of 96
74-4506 Micro SpinColumns, G-50, Qty. of 24
74-4502 Micro SpinColumns, G-50, Qty. of 96
74-3906 Macro SpinColumns, G-50, Qty. of 24
74-3902 Macro SpinColumns, G-50, Qty. of 96
74-5613 96-Well Micro SpinColumns, G-50, 1.1 ml Reservoir Plate (1)
74-5653 96-Well Macro SpinColumns, G-50, 1.1 ml Reservoir Plate (1)
74-7223 Ultra-Micro SpinColumns, G-100, Qty. of 24
74-7203 Ultra-Micro SpinColumns, G-100, Qty. of 96
74-4507 Micro SpinColumns, G-100, Qty. of 24
74-4503 Micro SpinColumns, G-100, Qty. of 96
74-3907 Macro SpinColumns, G-100, Qty. of 24
74-3903 Macro SpinColumns, G-100, Qty. of 96
74-5614 96-Well Micro SpinColumns, G-100, 1.1 ml Reservoir Plate (1)
74-5654 96-Well Macro SpinColumns, G-100, 1.1 ml Reservoir Plate (1)
74-7224 Ultra-Micro SpinColumns, P-2, Qty. of 24
74-7204 Ultra-Micro SpinColumns, P-2, Qty. of 96
74-4808 Micro SpinColumns, P-2, Qty. of 24
74-4802 Micro SpinColumns, P-2, Qty. of 96
74-4308 Macro SpinColumns, P-2, Qty. of 24
74-4302 Macro SpinColumns, P-2, Qty. of 96
74-5615 96-Well Micro SpinColumns, P-2, 1.1 ml Reservoir Plate (1)
74-5655 96-Well Macro SpinColumns, P-2, 1.1 ml Reservoir Plate (1)
74-7225 Ultra-Micro SpinColumns, P-6, Qty. of 24
74-7205 Ultra-Micro SpinColumns, P-6, Qty. of 96
74-4809 Micro SpinColumns, P-6, Qty. of 24
74-4803 Micro SpinColumns, P-6, Qty. of 96
74-4309 Macro SpinColumns, P-6, Qty. of 24
74-4303 Macro SpinColumns, P-6, Qty. of 96
74-5616 96-Well Micro SpinColumns, P-6, 1.1 ml Reservoir Plate (1)
74-5656 96-Well Macro SpinColumns, P-6, 1.1 ml Reservoir Plate (1)
74-3908 Macro SpinColumn, P-30, Qty. of 24
74-3892 Macro SpinColumn, P-30, Qty. of 96
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QuikPrep SpinColumns for gel filtration chromatography can be used for protein purification, buffer exchange, desalting, or for group separation.

Gel filtration, also called size-exclusion chromatography is an easy-to-use method for separation of molecules with different molecular sizes, using mild conditions. Gel Filtration uses the size of molecules in solution to determine separation.

SpinColumns have short media packing so the samples are separated by size, the large molecules travel out of the column with the void volume the smaller molecules, salts, etc. remain in the column. Desalting columns are used not only to remove low molecular weight contaminants such as salt, but also for buffer exchange before and after different chromatography techniques and for the rapid removal of reagents to terminate a reaction.

Examples of group separations include:

  • Removal of unincorporated nucleotides during DNA sequencing
  • Removal of free low molecular weight labels
  • Termination of reactions between macromolecules and low molecular weight reactants
  • Removal of products, cofactors or inhibitors from enzymes
  • Removal of unreacted radiolabels such as [α-32P]-ATP from nucleic acid labeling reactions

Available Matrices (Packing Materials)

Matrix Dia µm MWCO, Da Applications
 G-10*  40 to120  ≤700  Desalting peptides
 G-25*  40 to 120  ≤1,500  Desalting proteins and nucleic acids
 G-50*  50 to 150  100 to 5,000  Removal of free labels from labeled macromolecules
G-100*  40 to 150 1,000 to 10,000   Molecular weight determination 
 P-2**  45 to 90 100 to 1,800  Rapid carbohydrate and small peptide separations and desalting
 P-6**  45 to 90 1,000 to 6,000  Purification of proteins and polypeptides
P-30**  45 to 90  2,500 to 40,000  Purification of proteins

*Sephadex  **Polyacrylamide

For additional column specifications and technical information, please download our Guide to Gel Fitration or Size Exclusion Chromatography.

 

 

Specifications
Column Type Sample Volume Sample Capacity Suggested
Elution Volume
Included Choice of Packing Materials
(Matrices)
Ultra-Micro  10 µl to 25 µl 3 to 30 µg
 28.5 µl

Two 2 ml centrifuge tubes with top caps

G-10, G-25, G-50, G-100, P-2, P-6
Micro  25 µl to 75 µl 5 to 60 µg
 50 µl
Two 2 ml centrifuge tubes with top caps G-10, G-25, G-50, G-100, P-2, P-6
Macro  75 µl to 150 µl 30 to 300 µg
 143 µl
Two 2 ml centrifuge tubes with top and bottom caps G-10, G-25, G-50, G-100, P-2, P-6, P-30

96-Well Micro  25 µl to 75 µl 5 to
60 µg
 50 µl
Two 96-well collection plates (1.1 ml per well) G-10, G-25, G-50, G-100, P-2, P-6

96-Well Macro  25 µl to
150 µl
30 to
300 µg
 143 µl
Two 96-well collection plates (1.1 ml per well) G-10, G-25, G-50, G-100, P-2, P-6

G-10, -25, G-50, G-100 = Sephadex           
P-2, P-6, P-30 = Porous polyacrylamide

For additional column specifications and technical information, please download our Guide to Gel Fitration or Size Exclusion Chromatography.

DOCUMENT DESCRIPTION
QuikPrep Ultra Micro SpinColumn QSG 9511-042 Rev 2.0.pdf'Ultra-Micro SpinColumns Quick Start Guide
QuikPrep Micro SpinColumn QSG 9511-036 Rev 2.0-v1.pdf'Micro SpinColumns Quick Start Guide
QuikPrep Macro SpinColumn QSG 9511-032 Rev 2.0.pdf'Macro SpinColumns Quick Start Guide
QuikPrep 96 Well SpinColumns QSG 9511-033 Rev 2.0.pdf'96-Well SpinColumns Quick Start Guide