Fast Micro-Equilibrium Dialyzers

Fast Micro-Equilibrium Dialyzer

Reusable two-chamber system designed to be used in binding or interaction studies. For sample volumes from 25–1500 µl.

Membranes with molecular weight cut offs from 500 to 300,000 Da are available in regenerated cellulose, cellulose acetate and polycarbonate. (Sold separately.) Additional chambers (links) can also be purchased for more complex configurations. 

The Fast Micro-Equilibrium Dialyzer is made of inert PTFE allowing for high levels of sample recovery.  Each includes a body chamber, a link chamber, and two solid caps.



Item# Description U.S. List Price Quantity
7416-251D Fast Micro-Equilibrium Dialyzer, 25 µl Volume, Qty. of 1
7416-255D Fast Micro-Equilibrium Dialyzer, 25 µl Volume, Qty. of 5
7416-501D Fast Micro-Equilibrium Dialyzer, 50 µl Volume, Qty. of 1
7416-505D Fast Micro-Equilibrium Dialyzer, 50 µl Volume, Qty. of 5
7416-1001D Fast Micro-Equilibrium Dialyzer, 100 µl Volume, Qty. of 1
7416-1005D Fast Micro-Equilibrium Dialyzer, 100 µl Volume, Qty. of 5
7416-2501D Fast Micro-Equilibrium Dialyzer, 250 µl Volume, Qty. of 1
7416-2505D Fast Micro-Equilibrium Dialyzer, 250 µl Volume, Qty. of 5
7416-5001D Fast Micro-Equilibrium Dialyzer, 500 µl Volume, Qty. of 1
7416-5005D Fast Micro-Equilibrium Dialyzer, 500 µl Volume, Qty. of 5
7416-10001D Fast Micro-Equilibrium Dialyzer, 1000 µl Volume, Qty. of 1
7416-10005D Fast Micro-Equilibrium Dialyzer, 1000 µl Volume, Qty. of 5
7416-15001D Fast Micro-Equilibrium Dialyzer, 1500 µl Volume, Qty. of 1
7416-15005D Fast Micro-Equilibrium Dialyzer, 1500 µl Volume, Qty. of 5
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The Fast Micro-Equilibrium Dialyzer is a unique equilibrium dialysis chamber for small samples 25 to 1500 µl. It is ideally suited for binding assays or interaction studies. Two chambers with equivalent sample volumes are joined together with a membrane. In binding studies, one chamber contains a macromolecule while the other holds the ligand. The macromolecules are bound to their chamber while ligands are able to pass freely across the membranes to bind with the proteins. Once the chambers are at equilibrium samples can be extracted and the binding affinity determined. The entire assembly can be placed into a water bath for temperature controlled dialysis.

Membranes with molecular weight cut off from 500 to 300,000 Da are available to suit a wide range of applications. The large membrane surface to volume ratio allows for decreased dialysis times. The inert PTFE material allows for maximum sample retention, and allows the dialyzer unit to be autoclaved for reuse.

Each Fast Micro-Equilibrium Dialyzer includes a body chamber, a link chamber, and two solid caps. Additional links may be purchased for more complex configurations. Membranes are sold separately.

Advantages       

  • Easy to use
  • Leak-proof
  • Reusable
  • Inert to proteins
  • High sample recovery
  • Low protein binding
  • Autoclavable

Applications

  • Protein binding assays
  • Protein-drug binding assays
  • Receptor binding assays
  • Ligand binding assays
  • Protein-protein interactions
  • Protein-DNA interactions

 

Specifications7416-251D7416-255D7416-501D7416-505D7416-1001D7416-1005D7416-2501D7416-2505D7416-5001D7416-5005D7416-10001D7416-10005D7416-15001D7416-15005D
Filter Mediaµlµlµlµlµlµlµlµlµlµlµlµlµlµl
Packing Material252550501001002502505005001000100015001500