UP-100IH Core Systems

UP-100IH Core System

The UP-100IH is the preferred system for simple compound screening that requires  basic measured parameters such as aortic pressure, left ventricular pressure (isovolumetric LVP) and ECG Lead II measurement. The UP-100IH cannot be expanded to a working ejecting heart system.

The UP-100IH Core System, Basic is the more common configuration. It allows the researcher to measure two to three parameters in addition to those that are included in the standard system (aortic pressure and LVP). Most researchers will also want to operate in a constant pressure perfusion mode and measure coronary flow, which is a simple addition to the basic system. The basic system comes with a small amplifier housing, the advanced one with a full 19” rack for possible additional modules.  

The UP-100IH Core System, Advanced allows up to the full range of available measured parameters beyond the basic system including direct coronary flow, ECG lead II, temperature measurement and metabolic parameters (pH, pO2, pCO2). If your chosen options require more than 10 slot units, you need the Advanced system.

To ensure that your system is properly configured as a functional unit that meets your application needs, please complete our Checklist and then contact Technical Services at 800-547-6766 or via email at support@hbiosci.com before placing an order. In Europe, please call +49 7665 92000 or via email at sales@hugo-sachs.de. Upgrades can also be modified to meet your specific needs.

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Item# Description U.S. List Price Quantity
73-4385 UP-100IH Core System, Basic, 230 V
73-4384 UP-100IH Core System, Basic, 115 V
73-4387 UP-100IH Core System, Advanced, 230 V
73-4386 UP-100IH Core System, Advanced, 115 V
73-4388 Additions to UP-100IH Core System for Mouse Hearts (UP-MOUSE)
73-4389 Additions to UP-100IH Core System for Rat/Guinea Pig Hearts (UP-RAT/GP)
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The UP-100IH is the preferred system for simple compound screening that requires  basic measured parameters such as aortic pressure, left ventricular pressure (isovolumetric LVP) and ECG Lead II measurement. The UP-100IH cannot be expanded to a working ejecting heart system. 

The UP-100IH Core System, Basic is the more common configuration. It allows the researcher to measure two to three parameters in addition to those that are included in the standard system (Aortic Pressure and LVP). Most researchers will also want to operate in a constant pressure perfusion mode and measure coronary flow, which is a simple addition to the basic system. The basic system comes with a small amplifier housing, the advanced one with a full 19” rack for additional modules.  

The UP-100IH Core System, Advanced allows up to the full range of available measured parameters beyond the basic system including direct coronary flow, ECG lead II, temperature measurement and metabolic parameters (pH, pO2, pCO2). If your chosen options require more than 10 slot units, you need the Advanced system. The only difference between the Basic and Advanced system is the PLUGSYS case included.

Features & Benefits
Applications
Measured Signals & Calculated Parameters
Included items
Options and Upgrades

Features & Benefits

  • Most compact Langendorff design.
  • Using the SCP controller, constant pressure perfusion with no high water columns can be done.
  • Versatile perfusion system can be used for in situ or ex vivo perfusion of heart, liver, kidney and other organs and tissues.
  • Small bench footprint allows set up of multiple systems for increased throughput.
  • Ideal economic choice for the cost-conscious physiology/pharmacology lab with the need to screen drugs for safety pharmacology and toxicology in multiple organs.

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Applications

  • Drug compound screening
  • Safety pharmacology and toxicology
  • Study of myogene autoregulation with the addition of Direct Coronary Flow Measurement Option
  • Testing with inotropic substances
  • Testing of lusitrope substances
  • Testing of vasoactive substances
  • Cardiac rhythm tests
  • Ischemia/hypoxia studies
  • Cardioplegia studies
  • Toxicology studies
  • Biochemical tests

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Measured Signals and Calculated Parameters

The following signals are recorded as raw data:

  • Isovolumetric left ventricular pressure (balloon method)
  • Aortic (perfusion) pressure

The following parameters are calculated from the raw data (using a data acquisition system, e.g., ISOHEART):

  • dLVP/dt, dLVP/dt Max., dLVP/dt Min., contractility index
  • Systolic and diastolic LVP
  • Heart rate
  • Mean perfusion pressure
  • Indirect coronary flow measurement and constant pressure perfusion, with the addition of the SCP module

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Included Items

UP-100IH Core System, Basic, (73-4385) and Advanced (73-4387),
230 V include:
UP-100IH Core System, Basic, (73-4384) and Advanced (73-4386), 115 V include:
73-4228 Universal Perfusion System (UP-100) Base Unit* 73-4228 Universal Perfusion System (UP-100) Base Unit*
73-2070 Heart Chamber to UP-100 System (Size 3 Rodents) including Holder 73-2070 Heart Chamber to UP-100 System (Size 3 Rodents) including Holder
73-0720 Parts for Adaptation of the UP-100 for Retrograde Langendorff Heart Perfusion** 73-0720 Parts for Adaptation of the UP-100 for Retrograde Langendorff Heart Perfusion**
73-4544 Thermostatic Circulator TC120-ST5, Bath Volume 5 L, 230 V/50 Hz
73-4545  Thermostatic Circulator TC120-ST5, Bath Volume 5 L, 115 V/60 Hz
73-2448 REGLO Analog 2 Channel MS-2/8-160, 230 VAC, 50 Hz 73-2952 REGLO Analog 2 Channel MS-2/8-160, 115 VAC, 50 Hz
73-2068 Windkessel for Absorption of Pulsation Caused by Roller Pump 73-2068 Windkessel for Absorption of Pulsation Caused by Roller Pump

73-1521

73-0045

PLUGSYS Case, Type 601 (Basic)

PLUGSYS Case, Type 603 (Advanced)

73-1521

73-0045

PLUGSYS Case, Type 601 (Basic)

PLUGSYS Case Type 603 (Advanced)

73-3862 APT300 Pressure Transducers (2 included) 73-3862 APT300 Pressure Transducers (2 included)
73-1973 PLUGSYS Transducer Amplifier Module (TAM-D) 73-1973 PLUGSYS Transducer Amplifier Module (TAM-D)
73-0065 PLUGSYS Transducer Amplifier Modules (TAM-A) 73-0065 PLUGSYS Transducer Amplifier Modules (TAM-A)
73-3869 Holder for APT300 Transducer, 8 mm Rod, Length 75 mm 73-3869 Holder for APT300 Transducer, 8 mm Rod, Length 75 mm
73-0566 Perspex Block Clamp to mount transducer onto rod (up to 9 mm OD) 73-0566 Perspex Block Clamp to mount transducer onto rod (up to 9 mm OD)

* The UP-100 Base Unit (73-4228) includes plexiglas stand, heat exchanger with built-in bubble trap, holder for pressure transducer. (System volume = 30 ml)

** Parts included with 73-0720: aortic cannulae (4 different diameters), adapter for Luer cannulae with side port for pressure measurement, modification of holder for perfusion pressure measurement, rod and adaptation for LVP measurement

Required Species-Specific Additions

For a functional unit, the core system requires the addition of one of the options UP-MOUSE or UP-RAT/GP:

UP-MOUSE (73-4388) includes: UP-RAT/GP (73-4389) includes:
73-2798 Aortic Cannula with Luer Taper for Mouse to UP100-IH, PSCI or EasyCell, OD 1.0 mm  N/A (Required cannulae are included with the Core System as part of 73-2070)
73-0126 3-Stop Tygon® E-Lab Tubing, 1.22 mm ID, 12/pack, Red/Grey 73-0155 3-Stop Tygon® E-Lab Tubing, 2.79 mm ID, 12/pack, Purple/White
 73-3456  Tube Set for Jacketed Buffer Reservoir with Fluid Line Shutoff Valves
 73-3456  Tube Set for Jacketed Buffer Reservoir with Fluid Line Shutoff Valves
 73-3438  Reservoir Jacketed for Buffer Solution, with Frit, 1 L
 73-3440  Reservoir Jacketed for Buffer Solution, with Frit, 2 L
73-0331 Universal Mini Balloon Kit for LVP on Isolated Mouse Hearts  73-3560  Universal Balloon Kit V.2 for Rat/Guinea Pig Hearts
73-2787 Mouse/Neonatal Rat Ventricular Balloon Assembly Kit for Isovolumetric Contractile Force Measurements
 N/A  (10 Latex Balloons are included with 73-3560)


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Options & Upgrades

 If your chosen options require more than 10 Slot Units you will need to choose the UP-100IH Core System, Advanced.

Constant Pressure or Constant Flow Perfusion

Constant pressure perfusion is achieved by the optional addition of an SCP controller which modulates the flow generated by the perfusion pump based on a perfusion pressure feedback loop. The controller also provides an accurate, low-cost way to indirectly measure coronary flow. 

Coronary Flow Measurement Options for UP-100IH

The UP-100IH can be adapted for direct real-time coronary flow measurement with Ultrasound transit time technology. This allows measurement of fast flow changes, e.g. studying the myogene autoregulation (reactive hyperemia).

Alternatively coronary flow can be measured indirectly by controlling the pump speed of a roller pump  with the SCP controller.

Cell Isolation Upgrade for UP-100IH

For isolating cardiomyocytes following a physiology experiment or if you require occasional cardiomyocyte isolation in addition to the standard Langendorff experiment. This option forms a separate perfusion loop to avoid collagenase coming into the normal perfusion circuit. 

Oxygenating System Option for UP-100IH (for foaming solutions)

Choose this option if you are using buffer supplemented with albumin, fatty acids, washed erythrocytes, or other foaming additives.

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Specifications