UP-100IH Core Systems
The UP-100IH is the preferred system for simple compound screening that requires basic measured parameters such as aortic pressure, left ventricular pressure (isovolumetric LVP) and ECG Lead II measurement. The UP-100IH cannot be expanded to a working ejecting heart system.
The UP-100IH Core System, Basic is the more common configuration. It allows the researcher to measure two to three parameters in addition to those that are included in the standard system (aortic pressure and LVP). Most researchers will also want to operate in a constant pressure perfusion mode and measure coronary flow, which is a simple addition to the basic system. The basic system comes with a small amplifier housing, the advanced one with a full 19” rack for possible additional modules.
The UP-100IH Core System, Advanced allows up to the full range of available measured parameters beyond the basic system including direct coronary flow, ECG lead II, temperature measurement and metabolic parameters (pH, pO2, pCO2). If your chosen options require more than 10 slot units, you need the Advanced system.
To ensure that your system is properly configured as a functional unit that meets your application needs, please complete our Checklist and then contact Technical Services at 800-547-6766 or via email at support@hbiosci.com before placing an order. In Europe, please call +49 7665 92000 or via email at sales@hugo-sachs.de. Upgrades can also be modified to meet your specific needs.
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The UP-100IH is the preferred system for simple compound screening that requires basic measured parameters such as aortic pressure, left ventricular pressure (isovolumetric LVP) and ECG Lead II measurement. The UP-100IH cannot be expanded to a working ejecting heart system.
The UP-100IH Core System, Basic is the more common configuration. It allows the researcher to measure two to three parameters in addition to those that are included in the standard system (Aortic Pressure and LVP). Most researchers will also want to operate in a constant pressure perfusion mode and measure coronary flow, which is a simple addition to the basic system. The basic system comes with a small amplifier housing, the advanced one with a full 19” rack for additional modules.
The UP-100IH Core System, Advanced allows up to the full range of available measured parameters beyond the basic system including direct coronary flow, ECG lead II, temperature measurement and metabolic parameters (pH, pO2, pCO2). If your chosen options require more than 10 slot units, you need the Advanced system. The only difference between the Basic and Advanced system is the PLUGSYS case included.
Features & Benefits
Applications
Measured Signals & Calculated Parameters
Included items
Options and Upgrades
Features & Benefits
- Most compact Langendorff design.
- Using the SCP controller, constant pressure perfusion with no high water columns can be done.
- Versatile perfusion system can be used for in situ or ex vivo perfusion of heart, liver, kidney and other organs and tissues.
- Small bench footprint allows set up of multiple systems for increased throughput.
- Ideal economic choice for the cost-conscious physiology/pharmacology lab with the need to screen drugs for safety pharmacology and toxicology in multiple organs.
Applications
- Drug compound screening
- Safety pharmacology and toxicology
- Study of myogene autoregulation with the addition of Direct Coronary Flow Measurement Option
- Testing with inotropic substances
- Testing of lusitrope substances
- Testing of vasoactive substances
- Cardiac rhythm tests
- Ischemia/hypoxia studies
- Cardioplegia studies
- Toxicology studies
- Biochemical tests
Measured Signals and Calculated Parameters
The following signals are recorded as raw data:
- Isovolumetric left ventricular pressure (balloon method)
- Aortic (perfusion) pressure
The following parameters are calculated from the raw data (using a data acquisition system, e.g., ISOHEART):
- dLVP/dt, dLVP/dt Max., dLVP/dt Min., contractility index
- Systolic and diastolic LVP
- Heart rate
- Mean perfusion pressure
- Indirect coronary flow measurement and constant pressure perfusion, with the addition of the SCP module
Included Items
* The UP-100 Base Unit (73-4228) includes plexiglas stand, heat exchanger with built-in bubble trap, holder for pressure transducer. (System volume = 30 ml)
** Parts included with 73-0720: aortic cannulae (4 different diameters), adapter for Luer cannulae with side port for pressure measurement, modification of holder for perfusion pressure measurement, rod and adaptation for LVP measurement
Required Species-Specific Additions
For a functional unit, the core system requires the addition of one of the options UP-MOUSE or UP-RAT/GP:
| UP-MOUSE (73-4388) includes: | UP-RAT/GP (73-4389) includes: | ||
| 73-2798 | Aortic Cannula with Luer Taper for Mouse to UP100-IH, PSCI or EasyCell, OD 1.0 mm | N/A | (Required cannulae are included with the Core System as part of 73-2070) |
| 73-0126 | 3-Stop Tygon® E-Lab Tubing, 1.22 mm ID, 12/pack, Red/Grey | 73-0155 | 3-Stop Tygon® E-Lab Tubing, 2.79 mm ID, 12/pack, Purple/White |
| 73-3456 | Tube Set for Jacketed Buffer Reservoir with Fluid Line Shutoff Valves |
73-3456 | Tube Set for Jacketed Buffer Reservoir with Fluid Line Shutoff Valves |
| 73-3438 | Reservoir Jacketed for Buffer Solution, with Frit, 1 L |
73-3440 | Reservoir Jacketed for Buffer Solution, with Frit, 2 L |
| 73-0331 | Universal Mini Balloon Kit for LVP on Isolated Mouse Hearts | 73-3560 | Universal Balloon Kit V.2 for Rat/Guinea Pig Hearts |
| 73-2787 | Mouse/Neonatal Rat Ventricular Balloon Assembly Kit for Isovolumetric Contractile Force Measurements |
N/A | (10 Latex Balloons are included with 73-3560) |
Options & Upgrades
If your chosen options require more than 10 Slot Units you will need to choose the UP-100IH Core System, Advanced.
Constant Pressure or Constant Flow Perfusion
Constant pressure perfusion is achieved by the optional addition of an SCP controller which modulates the flow generated by the perfusion pump based on a perfusion pressure feedback loop. The controller also provides an accurate, low-cost way to indirectly measure coronary flow.
Coronary Flow Measurement Options for UP-100IH
The UP-100IH can be adapted for direct real-time coronary flow measurement with Ultrasound transit time technology. This allows measurement of fast flow changes, e.g. studying the myogene autoregulation (reactive hyperemia).
Alternatively coronary flow can be measured indirectly by controlling the pump speed of a roller pump with the SCP controller.
Cell Isolation Upgrade for UP-100IH
For isolating cardiomyocytes following a physiology experiment or if you require occasional cardiomyocyte isolation in addition to the standard Langendorff experiment. This option forms a separate perfusion loop to avoid collagenase coming into the normal perfusion circuit.
Oxygenating System Option for UP-100IH (for foaming solutions)
Choose this option if you are using buffer supplemented with albumin, fatty acids, washed erythrocytes, or other foaming additives.
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