Universal Perfusion System (UP-100)

The UP-100 is a multi-purpose perfusion system best utilized when different types of organs must be perfused either in situ or ex vivo. The modular design of this system allows easy adaptation for different applications using additions or extensions to the base unit.

Typical organs can be liver, kidney, mesenteric bed and heart of guinea pig, rat and mouse. The system also has been used for a bit more exotic perfusions of rabbit ear or rat hind limb. For in vivo perfusions an operating table is placed under the UP-100 stand. For ex vivo perfusions a moist chamber can be added, or for heart studies. a heart chamber.

Features & Benefits
Applications
System Extensions for Perfusions Ex Vivo
System Extensions for Perfusions In Situ
Included and Required Components

Features & Benefits

• Multipurpose system for perfusing isolated organs ex vivo or in situ
• Ideal for perfusing isolated organs such as: liver, rabbit ear, heart, kidney, rat hind limb, mesenteric bed
• Allows for constant flow or constant pressure perfusion

Applications

• Ex vivo perfusion of liver, kidney, mesenteric bed (requires the addition of a moist chamber)
  • Test of vasodilative drugs
  • Studying metabolic processes
  • Neural vascular tone
  • Organ preservation for transplant
  • General drug tests
  • Toxicology tests
• In situ perfusion of liver, kidney, mesenteric bed, hind limb, hindquarter (requires the addition of an operating table):
  
  • Blood vessel tone in peripheral vascular bed
  • Balance tests by muscle work (glucose/lactate/pyruvate, high energy phosphates/orthophosphate, etc.)
  • Test of vasodilative drugs in occlusive diseases of legs
  • Test of muscle relaxants (end-plate pharmacology)

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System Extensions for Perfusion Ex Vivo

Internal organs (kidney, liver, mesenteric bed) must be kept under optimal physiological conditions—moist and at defined temperature during perfusion. For these applications the UP-100 is combined with the Moist Chamber.

System Extensions for Perfusion In Situ

For in situ perfusion of organs such as liver and kidney, or for perfusion of regional vascular systems like hindquarter, an operating table (e.g. 73-3777, 73-3586 and 73-3976) can be placed on the main Plexiglas plate below the UP-100 mounting platform. The compact arrangement allows the connection line between organ and heat exchanger to remain short to ensure consistent perfusate temperature.

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Included and Required Components

Included items: Plexiglass stand, heat exchanger with built-in bubble trap, holder for APT300 pressure transducer, membrane system, pressure gauge and special spindle pump for perfusion pressure adjustment, membrane resistor (73-2316 only)

Additional equipment required: Moist Chamber or operating table, thermocirculator, cannulae, peristaltic pump, transducer for perfusion pressure measurement, monitoring system setup using the PLUGSYS Amplifier System e.g. perfusion pressure, flow, temperature, pH, pO₂, pCO₂. Recording and evaluation of the signals using BDAS or LabChart software.

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