Ultra Quiet Imaging Chamber (JG-23)

Ultra Quiet Imaging Chamber (JG-23)

JG-23 Series chambers have a special sloped surface and bath atrium that enables ultra quiet solution delivery.

  • Eliminates vibration associated with solution exchange
  • Incorporates Warner's diamond fluidics
  • Multiplexed solution delivery is possible without the potential of solution switching artifacts

Item# Description U.S. List Price Quantity
64-0255 Slice Anchor - Stainless Steel for RC-26G Chamber, 2.0 mm, SHD-26GH/2
64-0254 Slice Anchor - Stainless Steel for RC-26G Chamber, 1.5 mm, SHD-26GH/15
64-0253 Slice Anchor - Stainless Steel for RC-26G Chamber, 1.0 mm, SHD-26GH/10
64-0107 TA-29 Replacement Cable with Bead Thermistor
64-0106 Model CC-28 Cable Assemblies with Connector for TC-324B/344B
64-0755 PE-160/10 Polyethylene Tubing PE-160, 10 ft.
64-0708 CS-22/50 Coverslips for use with RC-26Z Chambers, 0.15 mm (0.006 in), 22 x 50 mm, pkg of 85
64-1506 (RPC-1) Replacement platform clamps and screws for P-1, P-3, P-4, P-5, and P-6 platforms, 2 pack
64-1526 PM-1 Chamber platform with magnetic clamps
64-0284 Series 20 Heater Platform, Model PH-1
64-0277 Series 20 Plain Platform, Model P-1
64-1488 Ultra Quiet Imaging Chamber (JG-23) low profile, wide bath
64-1487 Ultra Quiet Imaging Chamber (JG-23) high profile, wide bath
64-1486 Ultra Quiet Imaging Chamber (JG-23) high profile, narrow bath
Login for U.S. Pricing

The JG-23 has been designed especially for those researchers planning multiple solution changes while attempting to maintain a tight focus on the specimen during an optical experiment. The design of this chamber eliminates all vibrations associated with solution exchange that could cause specimens or optical fields to fluctuate during recordings.

The JG-23 incorporates the same advanced design as the Series 20 diamond-shaped bath chambers. A large imaging field allows for maximal observation of biological specimens with the addition of a sloped input. The advantages of this system over other chambers are that the sloped inflow allows for a smooth and 'ultra-quiet' delivery of solution to the chamber.

The input slope permits the use of I-V type infusion lines to deliver drops at a fixed rate to the chamber without the transfer of noise to the specimen field. In addition, multiplexed solution delivery is possible without the potential of solution switching artifacts.

JG-23 chambers require a platform ((PM-1, PH-1, or P-1) to complete the assembly for mounting onto a microscope.

A temperature controller TC-344C or TC-324C that connects to the heated platform can be added to set and maintain a certain temperature.

Perfusion can be achieved by adding a VC-8 or VC-6 perfusion system, the DVW vacuum system takes care of the waste.

Each chamber includes two magnetic plates, a magnetic perfusion tube holder, one pack of #1 thickness coverslips (CS-22/50, 22x50 mm, 85/pkg), and 10 ft of polyethylene (PE-150) tubing.

Slice anchors are available for both the JG-23W/HP and JG-23W/LP.

Material Polycarbonate Polycarbonate Polycarbonate
Bath dimensions (L x W) 6.3 x 21mm 15.2 x 24 mm 15.2 x 24 mm
Volume, by depth 128 µl* 238 µl* 238 µl*
Bottom coverglass 22 x 50 mm 22 x 50 mm 22 x 50 mm
OD (L x W x H) 50 x 23 x 4 mm 50 x 23 x 4 mm 50 x 23 x 2 mm

*Volumes are for 1 mm solution height


  1. Semi-automated region of interest generation for the analysis of optically recorded neuronal activity
    Mellen NM, Tuong CM
    Neuroimage. 2009 Oct 1;47(4):1331-40. Epub 2009 Apr 9
  2. A vibrating microtome attachment for cutting brain slice preparations at reproducible compound angles relative to the midline
    Mellen NM
    J Neurosci Methods. 2008 Feb 15;168(1):113-8. Epub 2007 Oct 2
  3. Lysophospholipids regulate excitability and exocytosis in cultured bovine chromaffin cells
    Pan CY, Wu AZ, Chen YT
    J Neurochem. 2007 Aug;102(3):944-56
  4. Neuroprotective potential of ceftriaxone in in vitro models of stroke
    Lipski J, Wan CK, Bai JZ, Pi R, Li D, Donnelly D
    Neuroscience. 2007 May 11;146(2):617-29. Epub 2007 Mar 23
  5. Functional Imaging Reveals Respiratory Network Activity During Hypoxic and Opioid Challenge in the Neonate Rat Tilted Sagittal Slab Preparation
    Barnes BJ, Tuong CM, Mellen NM
    J Neurophysiol. 2007 Mar;97(3):2283-92. Epub 2007 Jan 10
Integrated Components Brochure_06_7_11.pdfIntegrated Components for Live Cell Imaging Brochure
JG-23W Series (080724).pdfJW-23W Series Instructions
perfusion_strategies.pdfPerfusion Strategies (Leinders-Zufall)