Two Electrode Voltage Clamp Workstation (TEV-700)
This workstation provides many of the necessary components typically used with the OC-725C voltage clamp for research involving Oocytes.
The TEV-700 Workstation provides many of the necessary components typically used with the OC-725C voltage clamp for research involving Oocytes. When these components are purchased with the OC-725 Oocyte Clamp, the complete package is discounted, as shown below in the order information section.
BPM-1 - This solid steel plate provides a stable platform for mounting micromanipulators mounted on magnetic bases. A highly durable powder coating applied to the surfaces makes it impervious to most common lab spills.
Electrode Holders
Two vented electrode holders with silver wires are typically required with the clamp; a straight type for use with the voltage headstage and a 45° type with mounting handle for use with current electrodes. Vents have been added to the electrodes to prevent pressure build-up inside the electrode which can damage oocytes.
Select the holder based on your glass outer diameter. Unvented holders are available and have a 'N' at the end of the model number. Vented holders have a 'V' on the end of the model number. A two meter length cable assembly is provided to connect the current electrode holder to the clamp.
Holders are available separately.
System includes:
- Oocyte Clamp Amplifier; OC-725C; 64-0028
- Oocyte Recording Chamber; RC-3Z; 64-0319
- Left Micro-Manipulator; MM-33L; 64-0055
- Right Micro-Manipulator; MM-33R; 64-0056
- Magnetic Base (2 in complete system); MB/B; 64-0060
- Voltage and current electrode holders must be purchased separately
Optional magnetic steel base plate available; BPM-1; 64-1586
| Specifications |
|---|
| OC-725C Oocyte Clamp Specifications | ||
|---|---|---|
| Test Condition #1 | Model cell used to obtain specifications. Model membrane: 1 MΩ in parallel with 220 nF. Current and voltage electrodes both 1 MΩ impedence. | |
| Test Condition #2 | Noise measurements made with an 8-pole Bessel filter. | |
| Voltage Recording Channel (Vm) | V Probe Input Impedance | 0.5 x 1012 &, 1 pF |
| Output Resistance | 100 Ω | |
| DC Offset | ± 200 mV at input. Variations from zero with 10 turn control (20 mV/turn). | |
| Noise (0-10 kHz) | 3 µV RMS with input grounded. 20 µV RMS with model cell. | |
| Electrode Test | 10 mV/MΩ read on meter 100 mV/MΩ at Vm x10 output. | |
| Meter Range | ± 199.9 mV full scale | |
| Capacity Compen | 0 - 90 pF | |
| Current Sensing Channel (Bath Clamp) (Im) | Noise (0-1 kHz) | 4.5 nA RMS with bath clamp |
| 10 nA RMS in output leg | ||
| I Monitor Output | 1 nA/mV to 1 mA/10V in 7 steps and 3 ranges, x0.1, x1, and x10. | |
| Gain Telegraph Output | 0.2 to 2.6 V in 7 steps (200 mV/step) and 3 ranges, x0.1, x1, and x10. Compatible with data acquisition software* | |
| Meter Range, full scale | Clamp Current: ± 199.9 µA | |
| Electrode Voltage Ve | ± 199.9 mV; current meter reads Ve when clamp mode switch is off. | |
| Current Electrode Channel | Compliance Voltage | ± 180 V |
| Clamp Speed | 350 µsec; (10-90%) with 100 mV square wave command applied to model cell. | |
| Gain | Variable AC/DC: 0 to 2000 | |
| Fixed DC Gain | Switch selected: 1 x 106 | |
| Ve DC Offset | ± 200 mV at input (20 mV/turn) | |
| Electrode Test | 10 mV/MΩ read on current meter. | |
| 100 mV/MΩ at Ve x10 output rear panel | ||
| Commands | Hold | Manually set with digital potentiometer, 2 digit resolution and 2 ranges: |
| x1 range: ± 1 to 99 mV in 1 mV steps | ||
| x2 range: ± 2 to 198 mV in 2 mV steps | ||
| External | Signals applied to COMMAND IN÷10 1 V applied = 100 mV | |
| Physical Dimensions | Case | 8.9 cmx 43.2 cmx 30.5 cm |
| Voltage Headstage | 12.5 mm D x 5 cm L with 1.8 m cable | |
| Mounting Handle | 4.8 mm D x 6.3 cm L | |
| Bath Headstage | 2.3 cm x 3.5 cm x 4.2 cm with 1.8 m cable | |
| Shipping Weight | 6.8 kg | |
| Warranty | Three years, parts & labor | |
| Power Requirements | 100-130 V or 220-240 VAC, 50/60 Hz, 20 VA | |
| RC-3Z Oocyte Recording Chamber Specifications | ||
| Material | Polycarbonate | |
| Footprint, L x W | 13 x 7.7 cm (5.1 x 3 in) | |
| Bath Dimensions | ||
| Narrow Bath | 27 x 3.2 x 3.2 mm | |
| Volume | ~85 µl/mm Height | |
| Wide Bath | 27 x 6.3 x 3.2 mm | |
| Volume | ~170 µl/mm Height | |
| Chamber Floor Insert | 25 x 75 x 1 mm Polycarbonate | |
| Input Tubing Size | 1/16 in OD (PE-160) | |
| Output Tubing Size | 1/16 in ID | |
| DOCUMENT | DESCRIPTION |
|---|---|
| Connecting the OC-725 to pclamp (2005.06.24).pdf | Connecting the OC-725 to pClamp |