Small Volume Closed Bath Imaging Chambers (RC-20, RC-20H)

Small Volume Closed Bath Imaging Chambers (RC-20, RC-20H)

Polycarbonate chambers featuring fast perfusate exchange and linear solution flow.

  • Closed bath chamber design
  • Our smallest volume, closed bath chambers
  • Ideal for fluorescent, calcium, and time-lapse imaging studies

Item# Description U.S. List Price Quantity
64-0222 Small Volume Imaging Chamber, 48 ul
64-0223 Small Volume Imaging Chamber, 90 ul
64-0281 Series 20 Plain Platform, Model P-5
64-0288 Series 20 Heater Platform, Model PH-5
64-1564 PM-5 Chamber platform with magnetic clamps
64-1506 (RPC-1) Replacement platform clamps and screws for P-1, P-3, P-4, P-5, and P-6 platforms, 2 pack
64-0755 PE-160/10 Polyethylene Tubing PE-160, 10 ft.
64-0756 (PE-160/100) Polyethylene tubing, PE-160, 100 ft
64-0703 CS-15R Coverslips for use with RC-20, RC-20H, and RC-25F Chambers, 0.15 mm (0.006 in), 15 mm diameter, pkg of 100
64-0713 CS-15R15 Coverslips use with RC-20, RC-20H, RC-25F, RC-42LP, RC-37WC Chambers, 0.17 mm (0.0067 in), 15 mm diameter, pkg of 100
64-0276 Replacement Retaining Ring
64-0106 Model CC-28 Cable Assemblies with Connector for TC-324B/344B
64-0107 TA-29 Replacement Cable with Bead Thermistor
64-0274 Heater Elements (2) for PH (Heater) Platforms with Mounting Pads and Screws, RH-2
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The RC-20 and RC-20H are very small volume imaging chambers featuring fast solution exchange and short working distance. The smaller and narrower RC-20 insures linear solution flow and fast exchange while the RC-20H features a larger viewing area. An access port (Hamilton syringe compatible)* at the bath input may be used to inject substances into the solution flow or to remove gas bubbles.

Two 15 mm round coverslips enclose the bath. The distance between the top and bottom coverslips is 1 mm. The top coverslip is secured into place with a retainer ring.

Studies involving physiological bicarbonate buffers can be performed with these chambers with no gas loss at the chamber interface. In addition the closed design allows the use of permeable supports. With laminar flow, minimal distortion of the sample will occur during perfusion.

RC-20 and RC-20H chambers require a platform (PM-5,PH-5 or P-5) and stage adapter to complete the assembly for mounting onto a microscope.

chamber 2   chamber 1  chamber 3 
A temperature controller TC-344C or TC-324C that connects to the heated platform can be added to set and maintain a certain temperature. Perfusion can be achieved by adding a VC-8 or VC-6 perfusion system, the DVW vacuum system takes care of the waste.

Each chamber is supplied with a package of #1 thickness glass coverslips (CS-15R, 15 mm diameter, 100/pkg), 10 ft of PE-160 tubing, and a CR-15 top coverslip retaining ring.

* 25 Gauge (0.45 mm OD) needle

Material polycarbonate polycarbonate
Bath dimensions
(L x W)
11.5 x 6.3 mm 10.5 x 10.0 mm
Volume, by depth 36 μl/mm 70 μl/mm
Top coverglass 15 mm round 15 mm round
Bottom coverglass 15 mm round 15 mm round
Injection port
(ID x OD)
0.91 x 0.56 mm, stainless steel 0.91 x 0.56 mm, stainless steel
Input tubing
(ID x OD)
1.14 x 1.57 mm (PE-160) 1.14 x 1.57 mm (PE-160)
Output tubing
(ID x OD)
1.14 x 1.57 mm (PE-160) 1.14 x 1.57 mm (PE-160)

RC-20 drawing

  1. The Class II Phosphatidylinositol 3 kinase C2 Beta Is Required for the Activation of the K+ Channel KCa3.1 and CD4 T-Cells
    Srivastava S, Di L, Zhdanova O, Li Z, Vardhana S, Wan Q, Yan Y, Varma R, Backer J, Wulff H, Dustin ML, Skolnik EY
    Mol Biol Cell. 2009 Jul 8
  2. Temperature Compensation for Hybrid Devices: Kinesin's Km is Temperature Independent
    Tucker R, Saha AK, Katira P, Bachand M, Bachand GD, Hess H
    Small. 2009 Jun;5(11):1279-82
  3. Functional Cystic Fibrosis Transmembrane Conductance Regulator Expression in Cystic Fibrosis Airway Epithelial Cells by AAV6.2-Mediated Segmental Trans-Splicing
    Song Y, Lou H, Boyer J, Limberis M, Vandenberghe L, Hackett N, Leopold P, Wilson J, Crystal R
    Hum Gene Ther. 2008 Dec 12
  4. Protein histidine phosphatase 1 negatively regulates CD4 T cells by inhibiting the K+ channel KCa3.1
    Srivastava S, Zhdanova O, Di L, Li Z, Albaqumi M, Wulff H, Skolnik EY
    Proc Natl Acad Sci U S A. 2008 Sep 23;105(38):14442-6. Epub 2008 Sep 16
  5. Dynamics of Somatostatin Type 2A Receptor Cargoes in Living Hippocampal Neurons
    Lelouvier B, Tamagno G, Kaindl AM, Roland A, Lelievre V, Le Verche V, Loudes C, Gressens P, Faivre-Baumann A, Lenkei Z, Dournaud P
    J Neurosci. 2008 Apr 23;28(17):4336-49
  6. Response to Histamine Allows the Functional Identification of Neuronal Progenitors, Neurons, Astrocytes, and Immature Cells in Subventricular Zone Cell Cultures
    Agasse F, Bernardino L, Silva B, Ferreira R, Grade S, Malva JO
    Rejuvenation Res. 2008 Feb;11(1):187-200
  7. P2Y2 receptor desensitization on single endothelial cells
    Sanabria P, Ross E, Ramirez E, Colon K, Hernandez M, Maldonado HM, Silva WI, Jimenez-Rivera CA, Gonzalez FA
    Endothelium. 2008 Jan-Feb;15(1):43-51
  8. Plasmonic Nanosensors for Imaging Intracellular Biomarkers in Live Cells
    Kumar S, Harrison N, Richards-Kortum R, Sokolov K
    Nano Lett. 2007 May;7(5):1338-43. Epub 2007 Apr 17
  9. The unique cytoarchitecture of human pancreatic islets has implications for islet cell function
    Cabrera O, Berman DM, Kenyon NS, Ricordi C, Berggren PO, Caicedo A
    Proc Natl Acad Sci U S A. 2006 Feb 14;103(7):2334-9. Epub 2006 Feb 6
  10. Individual Mice Can Be Distinguished by the Period of Their Islet Calcium Oscillations
    Nunemaker CS, Zhang M, Wasserman DH, McGuinness OP, Powers AC, Bertram R, Sherman A, Satin LS
    Diabetes. 2005 Dec;54(12):3517-22
  11. Role for Hydrogen Peroxide in Flow-Induced Dilation of Human Coronary Arterioles
    Miura H, Bosnjak JJ, Ning G, Saito T, Miura M, Gutterman DD
    Circ Res. 2003 Feb 7;92(2):e31-40
Integrated Components Brochure_06_7_11.pdfIntegrated Components for Live Cell Imaging Brochure
RC-20 20H (080724).pdfRC-20 20H Chamber Instructions
perfusion_strategies.pdfPerfusion Strategies (Leinders-Zufall)