Fast Micro-Equilibrium Dialyzers (Reusable)

The QuikPrep^® Fast Micro-Equilibrium Dialyzer is a unique equilibrium dialysis chamber for small samples 25 to 1500 µl. It is ideally suited for binding assays or interaction studies. Two chambers with equivalent sample volumes are joined together with a membrane. In binding studies, one chamber contains a macromolecule while the other holds the ligand. The macromolecules are bound to their chamber while ligands are able to pass freely across the membranes to bind with the proteins. Once the chambers are at equilibrium samples can be extracted and the binding affinity determined. The entire assembly can be placed into a water bath for temperature controlled dialysis.

Membranes with molecular weight cut off from 500 to 300,000 Da are available to suit a wide range of applications. The large membrane surface to volume ratio allows for decreased dialysis times. The inert PTFE material allows for maximum sample retention, and allows the dialyzer unit to be autoclaved for reuse.

Each Fast Micro-Equilibrium Dialyzer includes a body chamber, a link chamber, and two solid caps. Additional links may be purchased for more complex configurations. Membranes are sold separately.

Advantages       

• Easy to use
• Leak proof
• Reusable
• Inert to proteins
• High sample recovery
• Low protein binding
• Autoclavable

Applications

• Protein binding assays
• Protein-drug binding assays
• Receptor binding assays
• Ligand binding assays
• Protein-protein interactions
• Protein-DNA interactions