Reverse Phase Chromatography SpinColumns
QuikPrep® SpinColumns™ for reverse phase chromatography are packed with a stationary non-polar phase. Available matrixes include: C4, C8, C18 and TARGA C18. See Description and Specifications for details.
QuikPrep® SpinColumns™ for reverse phase chromatography are packed with a stationary non-polar phase. Available matrixes include: C4, C8, C18 and TARGA C18.
Available Matrices (Packing Materials)
| Specifications | C18 | C8 | C4 | TARGA C18 |
| Particle Size, µm | 10 | 5 | 4.5 | 10 |
| Pore Size, À | 300 | 300 | 300 | 120 |
| Pore Volume, ml/gm | 0.9 | 0.9 | 0.9 | 0.8 |
| Surface Area, 100 m2/gm | 100 | 100 | 100 | 330 |
| % Carbon (w/w) | 8 | 5 | 3 | 18 |
| Silica Class | Type B | Type B | Type B | Type B |
| Acid and Alkali Stable | pH 1.5 to 10 | pH 1.5 to 10 |
pH 1.5 to 10 |
pH 1.5 to 10 |
C18, C8, C6 = Reverse phase bonding materials ofdifferent alkyl chain lengths
TARGA C18 = Monofunctional C18 phase with a unique polar and bulky end-capping
About Reverse Phase Chromatography
Reverse phase chromatography uses a column packed with a stationary non-polar phase, and a polar mobile phase is passed through the column. Molecules with similar polarities attract one another causing the most polar compounds to elute first as the non-polar compounds are held by the non-polar matrix These non-polar compounds can then be released through the addition of an elution buffer.
Available Matrices (Packing Materials)
| Specifications | C18 | C8 | C4 | TARGA C18 |
| Particle Size, µm | 10 | 5 | 4.5 | 10 |
| Pore Size, À | 300 | 300 | 300 | 120 |
| Pore Volume, ml/gm | 0.9 | 0.9 | 0.9 | 0.8 |
| Surface Area, 100 m2/gm | 100 | 100 | 100 | 330 |
| % Carbon (w/w) | 8 | 5 | 3 | 18 |
| Silica Class | Type B | Type B | Type B | Type B |
| Acid and Alkali Stable | pH 1.5 to 10 | pH 1.5 to 10 |
pH 1.5 to 10 |
pH 1.5 to 10 |
C18, C8, C4 = Reverse phase bonding materials of different alkyl chain lengths
TARGA C18 = Monofunctional C18 phase with a unique polar and bulky end-capping
SpinColumn Types
| Column Type | Sample Volume | Sample Capacity | Suggested Elution Volume |
Included | Choice of Packing Materials (Matrices) |
| Ultra-Micro | 10 µl to 25 µl | 3 to 30 µg |
28.5 µl |
Two 2 ml centrifuge tubes with top caps |
C4, C8, C18, TARGA C18 |
| Micro | 25 µl to 75 µl | 5 to 60 µg |
50 µl |
Two 2 ml centrifuge tubes with top caps | C4, C8, C18, TARGA C18 |
| Macro | 75 µl to 150 µl | 30 to 300 µg |
143 µl |
Two 2 ml centrifuge tubes with top and bottom caps | C4, C8, C-18, TARGA C18 |
| 96-Well Micro | 25 µl to 75 µl | 5 to 60 µg |
50 µl |
Two 96-well collection plates (1.1 ml per well) | C4, C8, C18, TARGA C18 |
| 96-Well Macro | 25 µl to 150 µl |
30 to 300 µg |
143 µl |
Two 96-well collection plates (1.1 ml per well) | C4, C8, C18, TARGA C18 |
