Normal Phase Chromatography SpinColumns

Normal Phase Chromatography SpinColumns

QuikPrep® SpinColumns™ for normal phase chromatography are packed with a stationary polar phase. Available matrixes include: Cyano (CN), Amino (NH2) and PHEA (Polyhydroxyethyl Aspartamide) and Silica. See Description and Specifications for details.

Normal phase chromatography uses a spin column packed with a stationary polar phase, and a non-polar mobile phase is passed through the column. Molecules with similar polarities attract one another causing the least polar compounds to elute first as the more polar compounds are held by the polar matrix. These polar compounds can then be released through the addition of an elution buffer.

Item# Description U.S. List Price Quantity
74-7231 Ultra-Micro SpinColumns, NH2, Qty. of 24
74-7211 Ultra-Micro SpinColumn, NH2, Qty. of 96
74-4611 Micro SpinColumn, NH2, Qty. of 24
74-4605 Micro SpinColumn, NH2, Qty. of 96
74-4111 Macro SpinColumn, NH2, Qty. of 24
74-4104 Macro SpinColumn, NH2, Qty. of 96
74-5622 96-Well Micro SpinColumn, NH2, 1.1 ml Reservoir Plate (1)
74-5662 96-Well Macro SpinColumns, NH2, 1.1 ml Reservoir Plate (1)
74-7230 Ultra-Micro SpinColumns, CN, Qty. of 24
74-7210 Ultra-Micro SpinColumn, CN, Qty. of 96
74-4610 Micro SpinColumn, CN, Qty. of 24
74-4604 Micro SpinColumn, CN, Qty. of 96
74-4110 Macro SpinColumn, CN, Qty. of 24
74-4106 Macro SpinColumn, CN, Qty. of 96
74-5621 96-Well Micro SpinColumn, CN, 1.1 ml Reservoir Plate (1)
74-5661 96-Well Macro SpinColumns, CN, 1.1 ml Reservoir Plate (1)
74-7232 Ultra-Micro SpinColumn, PHEA, Qty. of 24
74-7212 Ultra-Micro SpinColumn, PHEA, Qty. of 96
74-4811 Micro SpinColumn, PHEA, Qty. of 24
74-4805 Micro SpinColumn, PHEA, Qty. of 96
74-4311 Macro SpinColumn, PHEA, Qty. of 24
74-4305 Macro SpinColumn, PHEA, Qty. of 96
74-5623 96-Well Micro SpinColumn, PHEA, 1.1 ml Reservoir Plate (1)
74-5663 96-Well Macro SpinColumns, PHEA, 1.1 ml Reservoir Plate (1)
74-7229 Ultra-Micro SpinColumn, Silica, Qty. of 24
74-7209 Ultra-Micro SpinColumn, Silica, Qty. of 96
74-4606 Micro SpinColumn, Silica, Qty. of 24
74-4600 Micro SpinColumn, Silica, Qty. of 96
74-4105 Macro SpinColumn, Silica, Qty. of 24
74-4100 Maro SpinColumn, Silica, Qty. of 96
74-5620 96-Well Micro SpinColumn, Silica, 1.1 ml Reservoir Plate (1)
74-5660 96-Well Macro SpinColumns, Silica, 1.1 ml Reservoir Plate (1)
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QuikPrep® SpinColumns™ for normal phase chromatography are packed with a stationary polar phase. Available matrixes include: Cyano (CN), Amino (NH2) and Hydrophilic PHEA (Polyhydroxyethyl Aspartamide) and Silica.

Available Matrices (Packing Materials)

 Specifications NH CN  PHEA
 Particle Size, µm  25 to 40  25 to 40  12
 Pore Size, À  60 60  300
 Pore Volume, ml/gm  0.75  0.75  0.9
 Surface Area, 100 m2/gm  350  350  100
 % Carbon (w/w)  N/A  N/A  N/A
 Silica Class  Irregular  Irregular  Type B
 Acid and Alkali Stable  pH 2 to 8   pH 2 to 8
 pH 4 to 6.5

CN = Cyano   NH2 = Amino   PHEA = Polyhydroxyethyl Aspartamide (Hydrophilic) 

About Normal Phase Chromatography

Normal phase chromatography uses a column packed with a stationary polar phase, and a non-polar mobile phase is passed through the column. Molecules with similar polarities attract one another causing the least polar compounds to elute first as the polar compounds are held by the polar matrix. These polar compounds can then be released through the addition of an elution buffer

Specifications

Available Matrices (Packing Materials)

 Specifications NH CN  PHEA
 Particle Size, µm  25 to 40  25 to 40  12
 Pore Size, À  60 60  300
 Pore Volume, ml/gm  0.75  0.75  0.9
 Surface Area, 100 m2/gm  350  350  100
 % Carbon (w/w)  N/A  N/A  N/A
 Silica Class  Irregular  Irregular  Type B
 Acid and Alkali Stable  pH 2 to 8   pH 2 to 8
 pH 4 to 6.5

CN = Cyano   NH= Amino   PHEA = Polyhydroxyethyl Aspartamide (Hydrophilic) 


SpinColumn Types

Column Type Sample Volume Sample Capacity Suggested
Elution Volume
Included Choice of Packing Materials
(Matrices)
Ultra-Micro  10 µl to 25 µl 3 to 30 µg
 28.5 µl

Two 2 ml centrifuge tubes with top caps

Amino, Cyano, Hydrophilic PHEA Silica
Micro  25 µl to 75 µl 5 to 60 µg
 50 µl
Two 2 ml centrifuge tubes with top caps Amino, Cyano, Hydrophilic PHEA Silica
Macro  75 µl to 150 µl 30 to 300 µg
 143 µl
Two 2 ml centrifuge tubes with top and bottom caps Amino, Cyano, Hydrophilic PHEA Silica
96-Well Micro  25 µl to 75 µl 5 to
60 µg
 50 µl
One 96-well collection plate (1.1 ml per well) Amino, Cyano, Hydrophilic PHEA Silica
96-Well Macro  25 µl to
150 µl
30 to
300 µg
 143 µl
One 96-well collection plates (1.1 ml per well) Amino, Cyano, Hydrophilic PHEA Silica
DOCUMENT DESCRIPTION
QuikPrep Ultra Micro SpinColumn QSG 9511-042 Rev 2.0.pdf'Ultra-Micro SpinColumns Quick Start Guide
QuikPrep Micro SpinColumn QSG 9511-036 Rev 2.0-v1.pdf'Micro SpinColumns Quick Start Guide
QuikPrep Macro SpinColumn QSG 9511-032 Rev 2.0.pdf'Macro SpinColumns Quick Start Guide
QuikPrep 96 Well SpinColumns QSG 9511-033 Rev 2.0.pdf'96-Well SpinColumns Quick Start Guide