Reverse Phase Chromatography SpinColumns

Reverse Phase Chromatography SpinColumns

QuikPrep® SpinColumns™ for reverse phase chromatography are packed with a stationary non-polar phase. Available matrixes include: C4, C8, C18 and TARGA C18. See Description and Specifications for details.

Item# Description U.S. List Price Quantity
74-7242 Ultra-Micro SpinColumns, TARGA C18, Qty. of 24
74-7243 Ultra-Micro SpinColumns, TARGA C18, Qty. of 96
74-4613 Micro SpinColumns, TARGA C18, Qty. of 24
74-4614 Micro SpinColumns, TARGA C18, Qty. of 96
74-4115 Macro SpinColumns, TARGA C18, Qty. of 24
74-4116 Macro SpinColumns, TARGA C18, Qty. of 96
74-5637 96-Well Micro SpinColumn, TARGA C18, 1.1 ml Reservoir Plate (1)
74-5676 96-Well Macro SpinColumn, TARGA C18, 1.1 ml Reservoir Plate (1)
74-7226 Ultra-Micro SpinColumn, C18, Qty. of 24
74-7206 Ultra-Micro SpinColumn, C18, Qty. of 96
74-4607 Micro SpinColumn, C18, Qty. of 24
74-4601 Micro SpinColumns, C18, Qty. of 96
74-4107 Macro SpinColumn, C18, Qty. of 24
74-4101 Macro SpinColumn, C18, Qty. of 96
74-5617 96-Well Micro SpinColumn, C18, 1.1 ml Reservoir Plate (1)
74-5657 96-Well Macro SpinColumns, C18, 1.1 ml Reservoir Plate (1)
74-7227 Ultra-Micro SpinColumn, C8, Qty. of 24
74-7207 Ultra-Micro SpinColumn, C8, Qty. of 96
74-4608 Micro SpinColumns, C8, Qty. of 24
74-4602 Micro SpinColumn, C8, Qty. of 96
74-4108 Macro SpinColumn, C8, Qty. of 24
74-4102 Macro SpinColumn, C8, Qty. of 96
74-5618 96-Well Micro SpinColumn, C8, 1.1 ml Reservoir Plate (1)
74-5658 96-Well Macro SpinColumns, C8, 1.1 ml Reservoir Plate (1)
74-7228 Ultra-Micro SpinColumn, C4, Qty. of 24
74-7208 Ultra-Micro SpinColumn, C4, Qty. of 96
74-4603 Micro SpinColumn, C4, Qty. of 96
74-4609 Micro SpinColumn, C4, Qty. of 24
74-4109 Macro SpinColumn, C4, Qty. of 24
74-4103 Macro SpinColumn, C4, Qty. of 96
74-5619 96-Well Micro SpinColumn, C4, 1.1 ml Reservoir Plate (1)
74-5659 96-Well Macro SpinColumns, C4, 1.1 ml Reservoir Plate (1)
Login for U.S. Pricing


QuikPrep® SpinColumns™ for reverse phase chromatography are packed with a stationary non-polar phase. Available matrixes include: C4, C8, C18 and TARGA C18. 

Available Matrices (Packing Materials)

 Specifications C18  C8  C4  TARGA C18
 Particle Size, µm  10  5  4.5 10
 Pore Size, À  300 300   300 120
 Pore Volume, ml/gm  0.9  0.9  0.9 0.8
 Surface Area, 100 m2/gm  100  100  100 330
 % Carbon (w/w)  8  5  3 18
 Silica Class  Type B  Type B  Type B Type B
 Acid and Alkali Stable  pH 1.5 to 10   pH 1.5 to 10
 pH 1.5 to 10
pH 1.5 to 10

C18, C8, C6 = Reverse phase bonding materials ofdifferent alkyl chain lengths
TARGA C18 = Monofunctional C18 phase with a unique polar and bulky end-capping

About Reverse Phase Chromatography

Reverse phase chromatography uses a column packed with a stationary non-polar phase, and a polar mobile phase is passed through the column. Molecules with similar polarities attract one another causing the most polar compounds to elute first as the non-polar compounds are held by the non-polar matrix These non-polar compounds can then be released through the addition of an elution buffer.

Specifications

Available Matrices (Packing Materials)

 Specifications C18  C8  C4  TARGA C18
 Particle Size, µm  10  5  4.5 10
 Pore Size, À  300 300   300 120
 Pore Volume, ml/gm  0.9  0.9  0.9 0.8
 Surface Area, 100 m2/gm  100  100  100 330
 % Carbon (w/w)  8  5  3 18
 Silica Class  Type B  Type B  Type B Type B
 Acid and Alkali Stable  pH 1.5 to 10   pH 1.5 to 10
 pH 1.5 to 10
pH 1.5 to 10

C18, C8, C4 = Reverse phase bonding materials of different alkyl chain lengths
TARGA C18 = Monofunctional C18 phase with a unique polar and bulky end-capping

SpinColumn Types

Column Type Sample Volume Sample Capacity Suggested
Elution Volume
Included Choice of Packing Materials
(Matrices)
Ultra-Micro  10 µl to 25 µl 3 to 30 µg
 28.5 µl

Two 2 ml centrifuge tubes with top caps

C4, C8, C18, TARGA C18
Micro  25 µl to 75 µl 5 to 60 µg
 50 µl
Two 2 ml centrifuge tubes with top caps C4, C8, C18, TARGA C18
Macro  75 µl to 150 µl 30 to 300 µg
 143 µl
Two 2 ml centrifuge tubes with top and bottom caps C4, C8, C-18, TARGA C18
96-Well Micro  25 µl to 75 µl 5 to
60 µg
 50 µl
Two 96-well collection plates (1.1 ml per well) C4, C8, C18, TARGA C18
96-Well Macro  25 µl to
150 µl
30 to
300 µg
 143 µl
Two 96-well collection plates (1.1 ml per well) C4, C8, C18, TARGA C18
DOCUMENT DESCRIPTION
QuikPrep Ultra Micro SpinColumn QSG 9511-042 Rev 2.0.pdf'Ultra-Micro SpinColumns Quick Start Guide
QuikPrep Micro SpinColumn QSG 9511-036 Rev 2.0-v1.pdf'Micro SpinColumns Quick Start Guide
QuikPrep Macro SpinColumn QSG 9511-032 Rev 2.0.pdf'Macro SpinColumns Quick Start Guide
QuikPrep 96 Well SpinColumns QSG 9511-033 Rev 2.0.pdf'96-Well SpinColumns Quick Start Guide