Reverse Phase Chromatography SpinColumns

Reverse Phase Chromatography SpinColumns

QuikPrep SpinColumns for reverse phase chromatography are packed with a stationary non-polar phase. Available matrixes include: C4, C8, C18 and C18 Targa. See Description and Specifications for details.

Item# Description U.S. List Price Quantity
74-7242 Ultra-Micro SpinColumns, C18 Targa, Qty. of 24
74-7243 Ultra-Micro SpinColumns, C18 Targa, Qty. of 96
74-4613 Micro SpinColumns, C18 Targa, Qty. of 24
74-4614 Micro SpinColumns, C18 Targa, Qty. of 96
74-4115 Macro SpinColumns, C18 Targa, Qty. of 24
74-4116 Macro SpinColumns, C18 Targa, Qty. of 96
74-5637 96-Well Micro SpinColumn, C18 Targa, 1.1 ml Reservoir Plate (1)
74-5676 96-Well Macro SpinColumn, C18 Targa, 1.1 ml Reservoir Plate (1)
74-7226 Ultra-Micro SpinColumn, C18, Qty. of 24
74-7206 Ultra-Micro SpinColumn, C18, Qty. of 96
74-4607 Micro SpinColumn, C18, Qty. of 24
74-4601 Micro SpinColumns, C18, Qty. of 96
74-4107 Macro SpinColumn, C18, Qty. of 24
74-4101 Macro SpinColumn, C18, Qty. of 96
74-5617 96-Well Micro SpinColumn, C18, 1.1 ml Reservoir Plate (1)
74-5657 96-Well Macro SpinColumns, C18, 1.1 ml Reservoir Plate (1)
74-7227 Ultra-Micro SpinColumn, C8, Qty. of 24
74-7207 Ultra-Micro SpinColumn, C8, Qty. of 96
74-4608 Micro SpinColumns, C8, Qty. of 24
74-4602 Micro SpinColumn, C8, Qty. of 96
74-4108 Macro SpinColumn, C8, Qty. of 24
74-4102 Macro SpinColumn, C8, Qty. of 96
74-5618 96-Well Micro SpinColumn, C8, 1.1 ml Reservoir Plate (1)
74-5658 96-Well Macro SpinColumns, C8, 1.1 ml Reservoir Plate (1)
74-7228 Ultra-Micro SpinColumn, C4, Qty. of 24
74-7208 Ultra-Micro SpinColumn, C4, Qty. of 96
74-4603 Micro SpinColumn, C4, Qty. of 96
74-4609 Micro SpinColumn, C4, Qty. of 24
74-4109 Macro SpinColumn, C4, Qty. of 24
74-4103 Macro SpinColumn, C4, Qty. of 96
74-5619 96-Well Micro SpinColumn, C4, 1.1 ml Reservoir Plate (1)
74-5659 96-Well Macro SpinColumns, C4, 1.1 ml Reservoir Plate (1)
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QuikPrep SpinColumns for reverse phase chromatography are packed with a stationary non-polar phase. Available matrixes include: C4, C8, C18 and C18 Targa. 

 Specifications C18  C8  C4 
 Particle Size, µm  10  5  4.5
 Pore Size, À  300 300   300
 Pore Volume, ml/gm  0.9  0.9  0.9
 Surface Area, 100 m2/gm  100  100  100
 % Carbon (w/w)  8  5  3
 Silica Class  Type B  Type B  Type B
 Acid and Alkali Stable  pH 1.5 to 10   pH 1.5 to 10
 pH 1.5 to 10


About Reverse Phase Chromatography

Reverse phase chromatography uses a column packed with a stationary non-polar phase, and a polar mobile phase is passed through the column. Molecules with similar polarities attract one another causing the most polar compounds to elute first as the non-polar compounds are held by the non-polar matrix These non-polar compounds can then be released through the addition of an elution buffer.

Specifications74-724274-724374-461374-461474-411574-4116
Packing MaterialC18 Targa Packing MaterialC18 Targa Packing MaterialC18 Targa Packing MaterialC18 Targa Packing MaterialC18 Targe Packing MaterialC18 Targa Packing Material
Sample Size Metric10 to 2510 to 2525 to 7525 to 7575 to 15075 to 150
Sample Size Metric UOMµlµlµlµlµlµl
Column Type Sample Volume Sample Capacity Suggested
Elution Volume
Included Choice of Packing Materials
(Matrices)
Ultra-Micro  10 µl to 25 µl 3 to 30 µg
 28.5 µl

Two 2 ml centrifuge tubes with top caps

C4, C8, C18
Micro  25 µl to 75 µl 5 to 60 µg
 50 µl
Two 2 ml centrifuge tubes with top caps C4, C8, C18
Macro  75 µl to 150 µl 30 to 300 µg
 143 µl
Two 2 ml centrifuge tubes with top and bottom caps C4, C8, C-18
96-Well Micro  25 µl to 75 µl 5 to
60 µg
 50 µl
Two 96-well collection plates (1.1 ml per well) C4, C8, C18, C18 Targa
96-Well Macro  25 µl to
150 µl
30 to
300 µg
 143 µl
Two 96-well collection plates (1.1 ml per well) C4, C8, C18

C18, C8, C6= Reverse phase bonding materials of different alkyl chain lengths