Reverse Phase Chromatography SpinColumns

Reverse Phase Chromatography Spin Columns

These SpinColumn are packed with a stationary non-polar phase. Reverse phase matrixes include: C4, C8, C18 and C18 Targa. See Specifications for details.

Item# Description U.S. List Price Quantity
74-7228 Ultra-Micro SpinColumn, C4, Pkg. 24
74-7208 Ultra-Micro SpinColumn, C4, Pkg. 96
74-7227 Ultra-Micro SpinColumn, C8, Pkg. 24
74-7207 Ultra-Micro SpinColumn, C8, Pkg. 96
74-7226 Ultra-Micro SpinColumn, C18, Pkg. 24
74-7206 Ultra-Micro SpinColumn, C18, Pkg. 96
74-4609 Micro SpinColumn, C4, Pkg. 24
74-4603 Micro SpinColumn, C4, Pkg. 96
74-4608 Micro SpinColumns, C8, Pkg. 24
74-4602 Micro SpinColumn, C8, Pkg. 96
74-4607 Micro SpinColumn, C18, Pkg. 24
74-4601 Micro SpinColumns, C18, Pkg. 96
74-4109 Macro SpinColumn, C4, Pkg. 24
74-4103 Macro SpinColumn, C4, Pkg. 96
74-4108 Macro SpinColumn, C8, Pkg. 24
74-4102 Macro SpinColumn, C8, Pkg. 96
74-4107 Macro SpinColumn, C18, Pkg. 24
74-4101 Macro SpinColumn, C18, Pkg. 96
74-5619 96-Well Micro SpinColumn, C4, 1 plate
74-5618 96-Well Micro SpinColumn, C8, 1 Plate
74-5617 96-Well Micro SpinColumn, C18, 1 plate
74-5637 96-Well Micro SpinColumn, C18 Targa, 1 plate
74-5659 96-Well Macro SpinColumns, C4, 1 Plate
74-5658 96-Well Macro SpinColumns, C8, 1 Plate
74-5657 96-Well Macro SpinColumns, C18, 1 Plate
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These SpinColumn are packed with a stationary non-polar phase. Reverse phase matrixes include: C4, C8, C18 and C18 Targa.

 Specifications C18  C8  C4 
 Particle Size, µm  10  5  4,5
 Pore Size, À  300 300   300
 Pore Volume, ml/gm  0.9  0.9  0.9
 Surface Area, 100 m2/gm  100  100  100
 % Carbon (w/w)  8  5  3
 Silica Class  Type B  Type B  Type B
 Acid and Alkali Stable  pH 1.5 – 10   pH 1.5 – 10
 pH 1.5 – 10


About Reverse Phase Chromatography

Reverse phase chromatography uses a spin column packed with a stationary non-polar phase, and a polar mobile phase is passed through the column. Molecules with similar polarities attract one another causing the most polar compounds to elute first as the non-polar compounds are held by the non-polar matrix These non-polar compounds can then be released through the addition of an elution buffer.

Specifications
Column Type Sample Volume Bed Volume Sample Concentration Included Choice of Packing Materials (Matrices)
Ultra-Micro  10 µl - 25 µl 37.96 µl 3 - 30 µg

Two 2 ml centrifuge tubes with top caps

C-4, C-8, C18
Micro  25 µl - 75 µl 66.42 µl
5 - 60 µg
Two 2 ml centrifuge tubes with top caps C-4, C-8, C-18
Macro  75 µl - 150 µl 191.45 µl
30- 300 µg
Two 2 ml centrifuge tubes with top and bottom caps C-4, C-8, C-18
96-Well Micro  25 µl - 75 µl 66.42 µl
5 - 60 µg
Two 96-well collection plates (1.1 ml per well) C-4, C-8, C-18
96-Well Macro  25 µl - 150 µl 191.45 µl
30- 300 µg
Two 96-well collection plates (1.1 ml per well) C-4, C-8, C-18