ProFlow Shear Flow Chamber PFC-1

ProFlow Shear Flow Chamber PFC-1

The PFC-1 ProFlow chamber is based on the designs of Dr. Melissa Knothe Tate, currently of the Case Western Reserve University.

  • Computer designed gaskets optimized for well-defined, well-controlled shear-flow
  • Based on technology developed at Case Western Reserve University
  • Single- and dual-flow capable chamber
  • Made from polycarbonate for easy cleanup

Item# Description U.S. List Price Quantity
64-1860 ProFlow chamber, fit Series 30 stage adapters
64-1861 ProFlow gaskets, 0.250 mm thick, 10 pk
64-0713 CS-15R15 Coverslips use with RC-20, RC-20H, RC-25F, RC-42LP, RC-37WC Chambers, 0.17 mm (0.0067 in), 15 mm diameter, pkg of 100
64-0722 (CS-25R20) 25 mm round coverslip, glass, #2 thickness
64-0754 (PE-90/10) Polyethylene tubing, PE-90, 10 ft
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The chamber uses silicone gaskets, similar in approach to our popular RC-30 chamber, to form a closed-bath area sandwiched between two opposing glass coverslips. However, the ProFlow chamber uses specially designed gaskets that optimize the shear-flow dynamics within the bath. This optimized design results in a nearly uniform shear flow across the entire width of the bath. This allows the shear force to be more easily calculated when these gaskets are used.

Precut gaskets are manufactured from medical-grade silicone, are 0.250 mm thick, and can be stacked to achieve bath heights in multiples of a single gasket. Gaskets are supplied in packs of 10 and can be purchasedProFlow Diagram separately.

The system consists of a top plate and two bottom plates. One bottom plate allows the formation of a single-sided flow chamber for use with cultured cells, and the other bottom plate allows the formation of a double-sided flow chamber for use with isolated sections or for conditions where controlled flow is desired on both sides of a structure.

The PFC-1 is designed to fit into our Series 30 stage adapters.

Perfusion can be achieved by adding a VC-8 or VC-6 perfusion system, the DVW vacuum system takes care of the waste.

The PFC-1 comes complete with chamber top, two chamber bases, 0.250 mm thickness precut gaskets (10 pk), 15 mm round coverslips (# 1.5 thickness, 100 pk, CS-15R15), 25 mm round coverslips (#2 thickness, 50 pk, CS-25R20) and PE-90 tubing (10 ft).

Bath Dimensions22.0x10.4 mm (0.866 x 0.4 in)
Bath Volume47.5 ul
Gasket Thickness250 um (0.01 in)
Top Coverslip15 mm round, #1.5 thickness
Bottom Coverslip25 mm round, #2 thickness
Scallop on chamber bottom- single sided flow35.9 mm wide, 2.54 mm deep
1.44 in wide, 0.1 in deep
Scallop on chamber bottom- double sided flow25.9 mm wide, 5.53 mm deep
1.02 in wide, 0.22 in deep
Upper window size12.5 mm
Lower window size19.0 mm
Input/output Tubes10.6x0.67 mm ODxID (0.042x0.027 in)
Perfusion tubingPE-90, 0.86 mm ID (0.034 in)
Integrated Components Brochure_06_7_11.pdfIntegrated Components for Live Cell Imaging Brochure
PFC-1 (120127).pdfProFlow Shear Flow Chamber (PFC-1) User's Mnaual