Ponemah CARDIO Software
For over 3 decades Ponemah Software has been trusted by researchers worldwide to discover new insights into their research applications. Ponemah is the data acquisition platform that powers our DSI Implantable Telemetry solutions for cardiovascular research. Ponemah CARDIO software is tailored to acquire, visualize, and report cardiovascular endpoints. A wide range of essential cardiovascular metrics can be derived in real time from the primary signals acquired from in vivo models or ex vivo heart perfusion setups.
 Data acquisition, visualization, and analysis of in vivo or ex vivo cardiovascular hemodynamic and electrophysiologic signals.
 Accurate and consistent results through industry validated algorithms.
 Adjust algorithm settings to optimize signal analysis from various species and unique morphologies.
 Acquisition of primary signals from our DSI* ACQ7700 signal conditioners or HSE* PLUGSYS Modules as well as thirdparty analog signals.
 Builtin visualization tools to explore data and gain complete confidence in your results
 Fasttracks subsequent experiments by saving settings as a reusable Protocol
 Immediate results with realtime data visualization and analysis with visual validation marks.

Refine results by reanalyzing data segments posthoc and/or changing mark placement.
*Data Sciences International, Hugo Sachs Elektronik and Harvard Apparatus are all divisions of Harvard Bioscience, Inc.
To ensure that your system is properly configured as a complete setup that meets your experimental needs, please email us at sales@harvardapparatus.com or call us at 8005970580. In Europe, please call +49 7665 92000 or email sales@hugosachs.de. Upgrades can also be modified to meet your specific needs.
Additional Resources:
 Complete line of Ex Vivo Heart Perfusion Systems
 HSE/HA Tissue Bath Systems
 Isolated Lung or Abdominal Organ Perfusion Systems
 HSE PLUGSYS Cases and Modules
 HSE Data Acquisition Hardware
 DSI ACQ7700 Cases and Signal Conditioners
 Other Ponemah Platforms including CARDIO, PULMODYN, and BDAS
Data Analysis Modules Included with Ponemah CARDIO*
Each data analysis module derives a wide range of scientifically validated, industry approved cardiovascular endpoints.
 MultiLead Electrocardiogram (ECG)
 Blood Pressure (BP)
 Left Ventricular Pressure (LVP)
 Cardiac Volume (CVOL)
 Action Potential (MAP)
 Systemic Blood Flow (SBF)
 Coronary Blood Flow (CBF)
*Expand the specifications section below for a complete list of parameters derived from the primary signals
Reliable Data Services: We also offer Data Analysis as a service. We can assist with the creation of high quality, usable reports that summarize your experimental data and provide you with more time to evaluate the experimental results and plan your future studies. Contact us for more information.
 DSI Scientific Services for assistance in study setup and data analysis.
 Customized report packages for summarizing experimental data.
 Saves time, enhances confidence in results, and facilitates planning for future experiments.
MultiLead Electrocardiogram (ECG)
Analyzes ECG signals to provide single and multilead calculations. The ECG signal can have positive, negative, or biphasic T waves, P waves, and QRS complexes and validation marks, such as for Q, R, S, End of T and Beginning of P are automatically drawn.
MultiLead ECG parameters can also be calculated to provide additional information related to QT interval prolongation and dispersion.
The list below describes the parameters calculated either in realtime or during subsequent analysis.
Name  Definition 
Num  The number of the cardiac cycle. 
HR  The heart rate is computed in beatsperminute. 
RH, PH, TH  Height of the waves from the Isoelectric level, in millivolts. 
THN  Lowest point between the end of the S wave and the end of the T. 
STI  Time interval in milliseconds from the S wave to end of the following T wave. 
STE  The ST elevation, measured in “ST Measure” milliseconds after the S wave, from the Isoelectric level. 
QRS  Time interval of the QRS complex. 
PRI, QTI, RRI  Common Interval measurements. 
QAT  Q Alpha T is the time interval from the Q wave to the peak of the following T wave. 
QTcb, QTcf, QTcv, QTcm and QTck  Multiple corrected QT intervals (Bazett, Fridericia, Van de Water, Matsunaga and King). 
EQTS, EQTSc, EQTM, EQTMcs, EQTMce, QTMc  Various cross channel calculations available with Multilead ECG Analysis. 
QTD  QT Dispersion available with Multilead ECG Analysis. 
QRI, QRSA  QR interval, QR amplitude. 
MxdV  Maximum derivative of the R wave. 
TA  Area of the T wave from the Isoelectric level. 
PCt, TCt  The number of valid waves encountered in the logging period. 
QTCt  QT count. 
Count  Reports the number of ECG cycles in a given logging (averaging) period. 
BAD  The number of arrhythmic beats detected during a specified logging period. 
GW, TW  The Good Wave counts and number (Total Wave) of good and bad complexes. 
QATN  Time, in milliseconds, between the Q wave and the lowest point between the end of S and the end of T wave. 
PWdth  P Width reports the time, in milliseconds, between the start and end of the P wave. 
TpeI  The time in milliseconds between the peak of the T wave and the end of the T wave. 
TP  The signal value at the peak of the T wave relative to the Isoelectric level. 
Noise  This parameter reports an approximation of the noise level in the ECG cycle. 
Blood Pressure (BP) Analysis
Analyzes arterial and venous pressures. Pulse Wave Velocity calculations are also available when combined with a DSI or HSE hardwired solution using two pressure catheters.
In addition to displaying aortic pressure recordings, validation marks are automatically set for Diastolic, End Diastolic, Systolic. In addition, users can define and display % Recovery Point.
The list below describes the parameters calculated either in realtime or during subsequent analysis.
Name  Definition 
Num  The number of the cardiac cycle. 
Sys, Dia, Mean  Systolic, Diastolic, and Mean pressure. 
PH, HR, TTPK, ET  Common parameters include Pulse height, Heart rate, Time to peak, Ejection time. 
+dP/dt, dP/dt  Maximum positive and negative value of the first derivative of the pressure. 
%REC  The amount of time it takes the pressure to recover. 
NPMN  Nonpulsatile mean pressure. 
QA  The QA Interval is the time in milliseconds from the start of the Qwave, in the ECG trigger channel, to the start of the systolic pressure rise. 
Mean2  An alternate representation for Mean calculated as (Systolic + 2 * Diastolic)/3. 
PTT  Pulse Transit Time (PTT) is the time between the prior systolic time of the upstream channel and the systolic time of the selected channel. This time is reported in ms. 
PWV  Pulse Wave Velocity (PWV) is the velocity calculated by using the Pulse Wave Distance (PWD) and Pulse Transit Time (PTT). PWV is calculated as: Pulse Wave Velocity = Pulse Wave Distance / Pulse Transit Time. 
IBIs, IBIms, IBIed  InterBeat Interval (IBI) is the time in milliseconds between cardiac cycles and allows Heart Rate Variable (HRV) to be calculated from Blood Pressure signals (Frequency Domain). 
Count  Reports the number of cycles in a given logging period. 
Left Ventricular Pressure (LVP) Analysis
Analyzes pressure signals from the left ventricle and is used as a reference signal for other analysis modules, such as Cardiac Volume. Parameters such as Left Ventricular End Diastolic Pressure, Systolic Pressure, Percent Recovery Times and dP/dt max can be marked automatically.
The list below describes the parameters calculated either in realtime or during subsequent analysis.
Name  Definition 
Num  The number of the cardiac cycle. 
Sys  The systolic pressure is the maximum pressure that occurs during the cardiac cycle. 
LVEDP  The left ventricular end diastolic pressure is the pressure at the last zero crossing of the differentiated pressure during the rise to the systolic period. 
EMw  Electromechanical Window represents the time difference in ms between the end of electrical systole (end of the T wave) and the end of ventricular relaxation. It is a potential biomarker for Torsades de Pointes (TdP) risk that has greater predictability than using QT prolongation. 
Min  The minimum pressure during the cardiac cycle. 
TTI  TensionTime Index is the area under the left ventricular pressure during the ejection phase of the contraction. This is the integration between the LVEDP point and dP/dtMAX. 
DP  Developed pressure is the difference between the systolic pressure and the left ventricular end diastolic pressure (SYSLVEDP). 
HR  The heart rate is computed in beatsperminute. It is calculated by taking the reciprocal of the time interval for the cardiac cycle multiplied by 60. 
+dP/dt  +dP/dt is the maximum positive value of the first derivative of the pressure that occurs during the cardiac cycle. 
dP/dt  dP/dt is the maximum negative value of the first derivative of the pressure that occurs during the cardiac cycle. 
CI  Contractility index is +dP/dt divided by the pressure at that point. 
RT1, RT2  The Relaxation Time is the period from dP/dt to the time specified by the Relaxation Time attribute. The time is reported in milliseconds. 
dP (A, B, C, and D)  These parameters report the value of dP/dt at the pressure levels specified in dP/dt A, dP/dt B, dP/dt C, and dP/dt D (in the attributes window). 
NPMN  The nonpulsatile mean pressure reported for a logging period. This parameter is still reported even if no pulse pressure exists. 
QA  The QA Interval is the time in milliseconds from the start of the Qwave, in the ECG trigger channel, to the start of the systolic pressure rise (LVEDP). 
IVT  The time in milliseconds from the start of the systolic pressure rise (LVEDP) to the maximum slope of the systolic pressure rise (+dP/dt). 
TTIT  LVEDP to dP/dt. The time is in milliseconds. 
Tau  Tau is the time constant isovolumic left ventricular pressure decay. It is reported in milliseconds, and can be defined using Pressure, Developed Pressure and dP/dt. 
Period  The duration of the current cycle time, in milliseconds. 
Count  Reports the number of cycles in a given logging period. 
Cardiac Volume (CVOL) Analysis
Can analyze any volume from the circulatory system, as measured by a pressure volume catheter, and can derive on a beattobeat basis values for the cardiac cycle. Validation marks provide the user with visual documentation that the realtime analysis is providing appropriate cycle recognition.
Additional features built into the Data Review functionality allow the Cardiac Volume channel and LVP channel to be analyzed in a post process mode by the PressureVolume Loop analysis. PressureVolume Loops permit the display and analysis of left ventricular pressure cycles plotted against cardiac volume cycles.
PressureVolume Loop analysis features and calculations:
 Cuvette Calibration  Catheter Selection  Choose from 40 predefined PV catheters for volume calibration during acquisition.
 Parallel Volume calculation  Volume component due to parallel conductance. Parallel volume is subtracted from summed volume signal by means of a saline bolus injection or input of empirical data.
 Alpha Correction Factor  Reconcile the volume obtained using conductance technology with the volume obtained by other means. Stroke Volume is divided by Alpha.
 ESPVR (End Systolic PV Relationship)  Slope, X intercept, correlation coefficient.
 EDPVR (End Diastolic PV Relationship)  α, β, X intercept, correlation coefficient.
 PRSW (Pre Recruitable Stroke Work)  Slope, X intercept, correlation coefficient.
The list below describes the parameters calculated either in realtime or during subsequent analysis.
Name  Definition 
Num  The number of the cardiac cycle. 
Vmin  The minimum volume value in a cycle. 
Vmax  The maximum volume value in a cycle. 
SV  The stroke volume, which equals Vmax  Vmin. 
dPV  The volume at the LVP Min Slope mark. 
Ped  The pressure at the LVEDP mark. 
Ved  The volume at the LVEDP mark. 
%EF  Ejection fraction, calculated as Stroke Volume/Vmax * 100. 
PFR  Peak filling rate. 
dV  Peak emptying rate. 
TPFR  Time to peak filling rate. 
DFT  Diastolic Filling Time. 
%FT  Time to peak filling as a percentage of Diastolic fill time, equals TPFR/DFT * 100. 
Pes  Pressure at max elastance. 
Ves  Volume at max elastance. 
SW  Stroke Work. 
Ea  Arterial elastance. 
HR  Heart rate. Computed in beatsperminute. 
CO  Cardiac Output. 
Action Potential (MAP) Analysis
Analyzes a host of action potentials with focus on key regions such as plateau, recovery time and upstroke velocity.
The list below describes the parameters calculated either in realtime or during subsequent analysis.
Name  Definition 
Num  The number of the cardiac cycle. 
Max  The Maximum Voltage that occurred during a cardiac cycle. 
EDV  The voltage prior to the upstroke of the action potential. 
Min  The minimum voltage that occurred during a cardiac cycle. 
Plto  The plateau voltage. 
Amp  PLTO minus EDV. 
Rate  The beatsperminute. 
+dV  The maximum positive rate of change that occurred. 
dV  The maximum negative rate of change that occurred. 
REC1  The time, in milliseconds, from the beginning of the upstroke of the action potential, to the point where the signal drops below the level corresponding to the % Recovery 1 level. 
xR1  The number of cycles that did not reach the % Recovery 1 level, within the logging period. 
REC2  The time, in milliseconds, from the beginning of the upstroke of the action potential, to the point where the signal drops below the level corresponding to the % Recovery 2 level. 
xR2  The number of cycles that did not reach the % Recovery 2 level, within the logging period. 
REC3  The time, in milliseconds, from the beginning of the upstroke of the action potential, to the point where the signal drops below the level corresponding to the % Recovery 3 level. 
xR3  The number of cycles that did not reach the % Recovery 3 level, within the logging period. 
%Max  The time, in milliseconds, from the beginning of the upstroke of the action potential, to the point where the signal recovers to 100% or as close to 100% as possible. 
Time  The time interval between action potentials. 
Cnt  The number of cycles in the logging period. 
RiseT  The time, in milliseconds, between the EDV and Max points. 
Systemic Blood Flow (SBF) Analysis
Analyzes systemic blood flow from the circulatory system and eliminates drift resulting from certain types of flow meters.
The list below describes the parameters calculated either in realtime or during subsequent analysis.
Name  Definition 
Num  The number of the cardiac cycle. 
Mean  The integrated level of the flow for the cardiac cycle. 
FMax  Maximum Flow that occurred for a cardiac cycle. 
FMin  Minimum Flow that occurred for a cardiac cycle. 
CO  Cardiac Output is the mean flow normalized to ml/Min. Note: When running in a logging mode other than 1 epoch, the CO value will be calculated from the averaged mean flow value. 
SV  Stroke Volume. 
+dQ  +dQ is the maximum positive value of the first derivative of the flow that occurs during the cardiac cycle. 
Iso  The value of flow during the rapid up rise of the flow signal. 
TPR  Total Peripheral Resistance. 
NPMN  The nonpulsatile mean calculated over the complete logging interval. 
Coronary Blood Flow (CBF) Analysis
Provides coronary flow and volume information during systolic and diastolic periods in conjunction with the LVP module.
The list below describes the parameters calculated either in realtime or during subsequent analysis.
Name  Definition 
Num  The number of the cardiac cycle. 
Mean  The integrated level of the flow for the cardiac cycle. 
FMax  Maximum Flow that occurred for a cardiac cycle. 
FMin 
Minimum Flow that occurred for a cardiac cycle. 
CO  Cardiac Output. 
SV  Stroke Volume. 
+dQ  +dQ is the maximum positive value of the first derivative of the flow that occurs during the cardiac cycle. 
SFIw  Flow during systolic part of cardiac cycle. 
DFlw  Flow during diastolic part of cardiac cycle. 
SVol  Volume during the systolic part of the cardiac cycle. 
DVol  Volume during the diastolic part of the cardiac cycle. 
Flow  Any negative flow that occurred during the cardiac cycle. 
TVol  Total Volume is the sum of Systolic Volume and Diastolic Volume: SVol + DVol. 
NPMN  The nonpulsatile mean calculated over the complete logging interval. 