Small Preparation Dishes for Mouse Heart
The small preparation dish is used as a tool for isolated mouse heart cannulation, ideal to fix the mouse heart on the aortic cannula under a microscope.
There are three preparation dishes avalable for mouse heart:
- 73-0129: for IH-SR mouse cannulae only
- 73-4327: for Luer taper and IH-SR mouse cannulae
- 73-4464: for Luer taper cannulae only such as 73-2798
Due to the small diameter of the aorta of the mouse heart, it can often be challenging to accomplish a quick cannulation. If the process takes too long, the heart becomes ischemically compromised and must be discarded or risk confounding any experimental data.
The small preparation dish is used as a tool for isolated mouse heart cannulation, ideal to fix the mouse heart on the aortic cannula under a microscope.
There are three preparation dishes avalable for mouse heart:
- 73-0129: for IH-SR mouse cannulae only
- 73-4327: for Luer taper and IH-SR mouse cannulae
- 73-4464: for Luer taper cannulae only such as 73-2798
The preparation dish is placed on ice and filled with 4°C cold perfusion solution in order to keep metabolic function and ischemia low. It is designed with a very thin base so that when placed on an ice bath the perfusate in the dish will be dramatically cooled resulting in cardioprotective hypothermia.
The mouse cannula is held stable in the dish while the heart is mounted. An inlet port on the dish serves as the attachment point for a syringe filled with cold buffer that is used to fill the tube and the cannula bubble free and to carefully flush the blood from the heart. Over-pressurizing the heart is prevented by a pressure relief port just prior to the aortic cannula.
Once the heart is secured onto the cannula with suture and flushed, it can be removed from the preparation dish and mounted onto the perfusion system.
