UP-100IH Core Systems

The UP-100IH is a universal perfusion system adapted for basic Langendorff perfusion studies primarily in rat, guinea pig and juvenile rabbit heart. It can be used for mouse heart as well. It is the preferred system for simple compound screening that requires basic measured parameters such as aortic pressure, left ventricular pressure (isovolumetric LVP) and ECG Lead II measurement.

There are two UP-100IH Core Systems to choose from. These complete systems include all the primary equipment you need to accomplish the basic Langendorff experiment, only requiring the addition of species specific cannulae.

UP-100IH Core Systems

The UP-100IH Core Basic System is the more common configuration. It allows you to measure aortic pressure and left ventricular pressure (LVP), as well as two to three additional parameters of your choice. Most researchers will also want to operate in a constant pressure perfusion mode and measure coronary flow, which is a simple addition to the basic system.

The UP-100IH Core Advanced System allows up to the full range of available measured parameters beyond the aortic pressure and left ventricular pressure (LVP) that are included in the Basic Core System. Measurements such as direct coronary flow, ECG lead II, temperature and metabolic parameters (pH, pO₂, pCO₂) can easily be added.

The only difference between the Basic and Advanced Core System is the PLUGSYS case included. The Basic System comes with a small PLUGYS amplifier housing, the Advanced comes with a full PLUGSYS case that can be mounted on a 19” rack. If the options you need require more than 10 slot units, you will need to choose the Advanced Core System.

Advanced System Design
Features & Benefits
Applications
Measured Signals & Calculated Parameters
Included Items
Additional Requirements for a Functional Unit
Specialized Applications & Upgrades

Advanced System Design

The UP-100IH has a small footprint and small system volume  (<30 ml), making it ideal for multi-channel compound screening that can incorporate measurement of basic cardiac parameters such as  LVP, ECG and coronary flow.

The modular system can be additionally equipped with ECG lead II measurement, pacing, direct or indirect flow measurement, temperature measurement, metabolic monitoring, drug addition, and cell isolation.  The UP-100IH cannot be expanded to a working ejecting heart system.

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Features & Benefits  

• Versatile perfusion system can be outfitted in situ or ex vivo perfusion of heart, liver, kidney and other organs and tissues
• Constant pressure perfusion with no high water columns can be done using the SCP controller
• Small bench footprint allows set up of multiple systems for increased throughput
• Ideal economic choice for the cost-conscious physiology/pharmacology lab with the need to screen drugs for safety pharmacology and toxicology in multiple organs

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Applications

• Drug compound screening
• Safety pharmacology and toxicology
• Study of myogene autoregulation with the addition of Direct Coronary Flow Measurement Option
• Testing with inotropic substances
• Testing of lusitrope substances
• Testing of vasoactive substances
• Cardiac rhythm tests
• Ischemia/hypoxia studies
• Cardioplegia studies
• Toxicology studies
• Biochemical tests

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Measured Signals and Calculated Parameters

The following signals are recorded as raw data in retrograde Langendorff perfusion:

• Isovolumetric left ventricular pressure (balloon method)
• Aortic (perfusion) pressure

The following parameters are calculated from the raw data (using a data acquisition system, e.g., ISOHEART):

• dLVP/dt, dLVP/dt Max., dLVP/dt Min., contractility index
• Systolic and diastolic LVP
• Heart rate
• Mean perfusion pressure
• Indirect coronary flow measurement and constant pressure perfusion, with the addition of the SCP module

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Included Items

Included items are representative of a typical UP-100IH Core System. Individual components can be customized to your needs.

The Base Unit (73-4228) includes:

Plexiglas stand, heat exchanger with built-in bubble trap, holder for  pressure transducer and tubing for thermocirculator and perfusion circuit. (System volume = 30 ml). Cannulae not included.

Parts for Adaptation for Retrograde Langendorff Heart Perfusion (73-0720) include:

Aortic cannulae (four different diameters), adapter for Luer cannulae with side port for pressure measurement, modification of holder for perfusion pressure measurement, rod and adaptation for LVP measurement.

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Additional Requirements for a Functional Langendorff Unit

A UP100-IH functional unit requires the addition of species-specific cannulae. See table below.

For a functional unit, both core systems require the addition of  a species-specific option.

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Specialized Applications & Upgrades

Constant Pressure or Constant Flow Perfusion

Constant pressure perfusion is achieved by the optional addition of an SCP controller which modulates the flow generated by the perfusion pump based on a perfusion pressure feedback loop. The controller also provides an accurate, low-cost way to indirectly measure coronary flow. 

Coronary Flow Measurement

The UP-100IH can be adapted for direct real-time coronary flow measurement with Ultrasound transit time technology. This allows measurement of fast flow changes, e.g. studying the myogene autoregulation (reactive hyperemia). Alternatively coronary flow can be measured indirectly by controlling the pump speed of a roller pump  with the SCP controller.

Coronary Effluent Collection

For cannulating rat, guinea pig or small rabbit pulmonary artery for effluent sampling from a UP-100IH System. Used to sample the effluent of the pulmonary artery (effluent from the coronaries) for further analysis or for continuous pO₂, pH and pCO₂ measurements.

Perfusate Oxygenation

For oxygenation of buffers supplemented with albumin, fatty acids, washed erythrocytes or other foaming solutions. Normal oxygenation is done by bubbling in the reservoir. Foaming solutions cannot be bubbled and need a fiber oxygenator.

Cell Isolation Upgrade

For isolating cardiomyocytes in a UP-100IH System following a physiology experiment or for occasional cardiomyocyte isolation in addition to the standard Langendorff experiment. This option forms a separate perfusion loop to avoid collagenase coming into the normal perfusion circuit.

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