Perfusion Fast-Step (SF-77B)

Perfusion Fast-Step (SF-77B)

A simple but highly effective delivery device for stimulus solutions used in a variety of patch clamp and other electrophysiology applications.

  • Solution stimulus delivery for patch clamp and other electrophysiology studies
  • Solution changes in milliseconds
  • Minimal flow turbulence
  • No switching through intervening solutions
  • Manual or automatic step control (digital or analog)
  • Modest cost and easy maintenance
  • Use with most chambers or culture dishes

Item# Description U.S. List Price Quantity
64-0114 Model SF-77B Standard System with MM Series Manifolds, 1 pkg. 3SG700-5 Glass, GH-1 Glass Holder, and 1 pkg. each PE-10 and PE-50 Tubing
64-0117 (SF-77B/5M) Perfusion stepper system with 5 manifolds
64-0115 Model SF-77BST Theta Glass System with MM Series Manifolds, 1 pkg. TGC-200-10 Glass, GH-2T Glass Holder, and 1 pkg. each PE-10 and PE-50 Tubing
64-0116 Model SF-77BLT Large Tube System with ML Series Manifolds, 1 pkg. SG1000-5 Glass, GH-10 Glass Holder, and 2 pkg. of PE-50 Tubing
64-1515 Standard perfusion stepper system, 200-240 VAC, 50/60Hz
64-1515X6 (SF-77B) Standard perfusion stepper system, 200-240 VAC, 50/60Hz
64-1516 Perfusion stepper system, theta tubing, 200-240 VAC, 50/60Hz
64-1517 Perfusion stepper system, large tubing, 200-240 VAC, 50/60Hz
64-1518 (SF-77B/5M) Perfusion stepper system with 5 manifolds, 200-240 VAC, 50/60Hz
64-0174 Model VC-66MCS Complete Mini-Valve Perfusion System, 6-Channel
64-0119 Model 3SG700-5 3-Barrel Square Glass Tubes, 0.6 mm x 5 cm (ID x L), pkg. of 10
64-0120 Model 3SG700-10 3-Barrel Square Glass Tubes, 0.6 mm x 10 cm (ID x L), pkg. of 10
64-0121 Model SG-800-5 Single Barrel Square Glass Tubes,0.6 mm x 5 cm (ID x L), pkg. of 25
64-0122 Model SG-1000-5 Single Barrel Square Glass Tubes, 1 mm x 5 cm (ID x L), pkg. of 25
64-0811 Premium Theta Glass, Model TG200-4, Pkg. of 100
64-0203 Model MM-2 Perfusion Manifold, 2 Inputs
64-0204 Model MM-4 Perfusion Manifold, 4 Inputs
64-0205 Model MM-6 Perfusion Manifold, 6 Inputs
64-0124 Model GH-1 Glass Holder for 3SG700-5, 3SG700-10 and SG800-5 Glass
64-0125 Model GH-2T Glass Holder for Theta Glass
64-0126 Model GH-10 Glass Holder for SG1000-5 Glass
64-0752 PE-50/10 PE-50 Polyethylene Tubing, 10 ft L
64-0753 Model PE-50/100 PE-50 Polyethylene Tubing, 100 ft L
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The SF-77B is a simple but highly effective stimulus solution delivery device for use in a variety of patch clamp and electrophysiology studies. Control and test solutions flow continuously through adjacent stimulus delivery tubes and a stepper mechanism selects which tube is directed at the preparation. The rapid response and nominal hysteresis of the stepper mechanism allows for very fast switching times. Complete solution changes are typically achieved within 20 msec for a large 700 mm step and times are significantly shortened as the step size is decreased.

Multiple Solution Studies
In the standard configuration, up to six different solutions are connected to a single input manifold which in turn is connected to one of three square glass stimulus ports. The three stimulus port design is superior to a two-port design in that complex solution exchange protocols can be brought to bear on the sample under study. Since the complete system is designed to accommodate three manifolds (one for each stimulus port) and each manifold can accommodate up to 6 feed lines, it is possible to immediately select between 18 different input solutions.


  • Solution changes between stimulus ports occur within milliseconds
  • Changes between solutions connected to individual port occur within 5 secs
  • Entirely new solutions can be added into any port with a waiting time of no more than 30 seconds
  • Cell is never required to pass through intervening solutions to get from control to test solution

Manual or External Control
The stepper mechanism can be manually controlled via the front panel or externally directed from your data acquisition program. Manually, the system can be stepped to 8 positions in 7 equally spaced steps. These same 8 positions can also be directly selected by applying an analog signal to the external analog input BNC or by passing a 3 byte word to the TTL input on the instrument rear panel.

Square Glass Stimulus Ports
The square glass tubes used for solution delivery significantly reduces mixing turbulence between solution flows allowing the SF-77B to be used for studies with both membrane patches and whole cells, even when the cells are not fixed to a substrate.

System Versatility
The design of the SF-77B permits the use of various size glass tubing for perfusion delivery.

SF-77B Standard System (0.7 mm ID tubes):
This standard system is shipped with 3SG700-5 single-walled 3-barrel glass tubing which eliminates the need to glue individual barrels together. Spacing between barrels is 0.7 mm and step speed between adjacent barrels is typically 20 msec. Single barrel SG800-5 tubes (up to 5) can be used with the same holder.

SF-77B/5M Standard System with 5 tubes (0.7 mm ID tubes):
This is the same package as the standard system except that the manifold bracket and tube holder have been extended to accept 5 single tubes and associated perfusion lines. The individual tubes can be glued together to make a larger assembly, if desired. Spacing between barrels is 0.6 mm and step speed between adjacent barrels is typically less than 20 msec. Also included are four (4) 6-to-1 MM Series manifolds and one (1) 2-to-1 MM Series manifold.

Large Tube System
(1.0 mm ID tubes) Larger stimulus ports are required when using the SF-77B with larger cell structures such as the Xenopus oocyte. Solutions are delivered through 1.0 mm ID square tubes (SG1000-5) with barrel-to-barrel spacing of 1.4 mm.

1 msec Stepping With Theta Tubing
The SF-77B has been successfully tested using theta style capillary tubing with a step speed of 1 msec between adjacent barrels. Standard 2 mm diameter theta tubing is pulled to a tip diameter of 300 um with a spacing of 100 um between barrels. In this design, placement of the pipette tip is critical due to the smaller perfusion stream. Since mechanical artifacts can be evident when using these small step sizes the motor drive voltage is adjustable at the rear panel.

Easy Set-Up
The stepper mechanism is compact, lightweight, and free of either mechanical or electrical noise. The mechanism connects to the control box with a 2 meter shielded cable and is provided with a mounting rod for attachment to a manipulator. Manifolds can support 2,4 or 6 inputs depending on the experiment. Solutions flow from reservoirs to the manifold through PE-50 tubing and PE-10 tubing is used to connect the manifold outputs to the glass tubes.

We designed the VC-6M to accompany the SF-77B stepper. It conveniently fits inside a Faraday enclosure (e.g. FC-0, FC-1, FC-2) and is remotely controlled with the VC-6 controller. When purchased with the SF-77B, the complete package is discounted. These Fast-Step Systems are supplies with one 2 to 1 and two 6 to 1 manifolds. Other manifolds are available separately.

Number of Steps 1 to 7 (2 to 8 positions)
Step Size Adjustable from 100 µm to 1.5 mm in 100 µm increments, with STEPS/POSITION switch
Step Speed Typically 20 msec for 700 µm step
Step Control
Manual 8 positions with POSITION selector
Analog Signal 5 positions with voltage levels 0-4 V, 1V per step
Digital Signal 8 positions with 3 byte TTL signal
Max. Stepped Range 12.5 mm
Mounting Handle 8 mm X 10 cm (D x L)
Stepper Weight 110.5 g (including handle)
Three manifolds supplied
MM series for SF-77B and SF-77BST
ML series with SF-77 BLT
Manifolds have 2, 4 or 6 inputs
One two input and two six input manifolds are supplied
MM Series MM-2, MM-4 and MM-6 manifolds use PE-50 tubing at input and PE-10 tubing at output
ML Series ML-2, ML-4 and ML-6 manifolds use PE-50 tubing at both input and output
Solution Flow Rates Rates measured with solution reservoir height of approx. 60 cm (24 in)
With MM Series 100 µL/min
With ML Series 1 mL/min
Control Box
Size, H x W x D 6.3 x 14 x 23 cm
Power 100 to 130 or 220 to 250 VAC, 50/60 Hz, 10 VA
Shipping Weight 2.7 kg
Warranty 2 years, parts and labor
  1. A new culturing strategy improves functional neuronal development of human neural progenitor cells
    Schaarschmidt G, Schewtschik S, Kraft R, Wegner F, Eilers J, Schwarz J, Schmidt H
    J Neurochem. 2009 Apr;109(1):238-47
  2. State-dependent modulation of CFTR gating by pyrophosphate
    Tsai MF, Shimizu H, Sohma Y, Li M, Hwang TC
    J Gen Physiol. 2009 Apr;133(4):405-19
  3. Roles of GRK and PDE4 activities in the regulation of beta2 adrenergic signaling.
    Xin W, Tran TM, Richter W, Clark RB, Rich TC.
    J Gen Physiol. 2008 Apr;131(4):349-64. Epub 2008 Mar 17.
  4. Stabilization of cardiac ryanodine receptor prevents intracellular calcium leak and arrhythmias
    Lehnart SE, Terrenoire C, Reiken S, Wehrens XH, Song LS, Tillman EJ, Mancarella S, Coromilas J, Lederer WJ, Kass RS, Marks AR
    Proc Natl Acad Sci U S A. 2006 May 16;103(20):7906-10
  5. Cross talk between activation and slow inactivation gates of Shaker potassium channels
    Panyi G, Deutsch C,
    J Gen Physiol (2006) 128:547-59
  6. The Block of CFTR by Scorpion Venom is State-Dependent
    Fuller MD, Zhang ZR, Cui G, McCarty NA
    Biophys J. 2005 Dec;89(6):3960-75
  7. Neuroprotection in ischemia: blocking calcium-permeable acid-sensing ion channels
    Xiong ZG, Zhu XM, Chu XP, Minami M, Hey J, Wei WL, MacDonald JF, Wemmie JA, Price MP, Welsh MJ, Simon RP
    Cell. 2004 Sep 17;118(6):687-98
  8. Nociceptor and Hair Cell Transducer Properties of TRPA1, a Channel for Pain and Hearing
    Nagata K, Duggan A, Kumar G, García-Añoveros J
    J Neurosci. 2005 Apr 20;25(16):4052-61
  9. Tonic inhibition in mouse hippocampal CA1 pyramidal neurons is mediated by ?5 subunit-containing ?-aminobutyric acid type A receptors
    Caraiscos VB, Elliott EM, You-Ten KE, Cheng VY, Belelli D, Newell JG, Jackson MF, Lambert JJ, Rosahl TW, Wafford KA, MacDonald JF, Orser BA
    Proc Natl Acad Sci U S A. 2004 Mar 9;101(10):3662-7
  10. Activity-dependent bidirectional regulation of GABAA receptor channels by the 5-HT4 receptor-mediated signalling in rat prefrontal cortical pyramidal neurons
    Cai X, Flores-Hernandez J, Feng J, Yan Z
    J Physiol (2002) 540.3: 743.759
  11. NMDA receptor function in mouse models of Huntington disease
    Cepeda C, Ariano MA, Calvert CR, Flores-Hernández J, Chandler SH, Leavitt BR, Hayden MR, Levine MS
    J Neurosci Res. 2001 Nov 15;66(4):525-39
  12. Structural Domains of the CB1 Cannabinoid Receptor That Contribute to Constitutive Activity and G-Protein Sequestration.
    Nie J, Lewis DL,
    J Neurosci (2001) 21:8758.8764
  13. Allosteric interaction between the amino terminal domain and the ligand binding domain of NR2A.
    Zheng F, Erreger K, Low C-M, Banke T, Lee CJ, Conn PJ, Traynelis SF,
    Nature Neurosci (2001) 4:894-901
  14. A uniform extracellular stimulus triggers distinct cAMP signals in different compartments of a simple cell
    Rich TC, Fagan KA, Tse TE, Schaack J, Cooper DM, Karpen JW
    Proc Natl Acad Sci U S A. 2001 Nov 6;98(23):13049-54
  15. Vanilloid receptor-1 is essential for inflammatory thermal hyperalgesia
    Davis JB, Gray J, Gunthorpe MJ, Hatcher JP, Davey PT, Overend P, Harries MH, Latcham J, Clapham C, Atkinson K, Hughes SA, Rance K, Grau E, Harper AJ, Pugh PL, Rogers DC, Bingham S, Randall A, Sheardown SA
    Nature. 2000 May 11;405(6783):183-7
  16. The endogenous lipid anandamide is a full agonist at the human vanilloid receptor (hVR1).
    Smart D, Gunthorpe MJ, Jerman JC, Nasir S, Gray J, Muir AI, Chambers JK, Randall AD, Davis JB,
    Brit J Pharmacol (2000) 129:227-30
  17. Permeation and Activation of the M2 Ion Channel of Influenza A Virus.
    Mould JA, Drury JE, Frings SM, Kaupp UB, Pekosz A, Lamb RA, Pinto LH,
    J Biol Chem (2000) 275:31038.50
  18. Functional Expression of a Mammalian Odorant Receptor.
    Zhao H, Ivic L, Otaki JM, Hashimoto M, Mikoshiba K, Firestein S,
    Science (1998) 279:237-242
  19. Selectivity Changes during Activation of Mutant Shaker Potassium Channels
    Zheng J, Sigworth FJ
    J Gen Physiol (1997) 110:101.117
  20. Molecular Characterization of Human and Rat RGS 9L, a Novel Splice Variant Enriched in Dopamine Target Regions, and Chromosomal Localization of the RGS 9 Gene.
    Granneman JG, Zhai Y, Zhu Z, Bannon MJ, Burchwtt SA, Schmitt CJ, Andrade R, Cooper J,
    Mol Pharmacol (1989) 54:687-94
SF-77B (080908).pdfPerfusion Fast Step (SF-77B) Manual