Open Round Bath Imaging Chamber (RC-21BRW)

Open Round Bath Imaging Chamber (RC-21BRW)

This recording chamber features a round, wide aperture, open bath for maximum imaging area.

  • Designed for physiological measurements on cell cultured coverslips
  • Uses a 25 mm diameter round coverslip as the chamber floor
  • Quiet operation
  • Broad imaging area
  • Wicked solution removal from bath
  • Can be used with upright or inverted microscopes

Item# Description U.S. List Price Quantity
64-0227 Open Chamber for 25 mm Round Coverslip, 240 ul, RC-21BRW
64-0278 Series 20 Plain Platform, Model P-2
64-0285 Series 20 Heater Platform, Model PH-2
64-1561 PM-2 Chamber platform with magnetic clamps
64-0755 PE-160/10 Polyethylene Tubing PE-160, 10 ft.
64-0756 (PE-160/100) Polyethylene tubing, PE-160, 100 ft
64-0705 CS-25R Coverslips, 0.15 mm (0.006 in), 25 mm diameter, pkg of 100
64-0106 Model CC-28 Cable Assemblies with Connector for TC-324B/344B
64-0107 TA-29 Replacement Cable with Bead Thermistor
64-0274 Heater Elements (2) for PH (Heater) Platforms with Mounting Pads and Screws, RH-2
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The RC-21BRW is an open bath recording chamber using a 25 mm diameter round coverslip as the chamber bottom. The wide, round aperture of the RC-21BRW maximizes the viewing area on the coverslip and enables excellent access for electrodes. Constant solution level and quiet operation are assured with the use of a wick between the bath and suction well. Bath volume is 240 µl per mm solution height.

The low profile design of the RC-21BRW is ideal for use with large diameter objectives and will accommodate either upright or inverted microscopes. The large imaging area (17 mm diameter) increases the likelihood of viewing desired cell features located around the periphery of the chamber.

RC-21BRW chambers require a platform (PM-2, PH-2, or P-2) and a stage adapter to complete the assembly for mounting onto a microscope.


A temperature controller TC-344C or TC-324C that connects to the heated platform can be added to set and maintain a certain temperature.

Perfusion can be achieved by adding a VC-8 or VC-6 perfusion system, the DVW vacuum system takes care of the waste.

Each chamber includes a pack of #1 thickness glass coverslips (CS-25R, 25 mm diameter, 100/pkg) and 10 ft of PE-160 tubing.

An application video is available for this product through the JoVE website!
You can view our selection of videos and access full length videos

Material Polycarbonate
Bath dimension (D x H) 17.4 mm x 2.5 mm
Volume, by depth 240 μl/mm
Bottom coverglass 25 mm round
Injection port
(ID x OD)
0.91 x 0.56 mm, stainless steel
Input tubing
(ID x OD)
1.14 x 1.57 mm (PE-160)
Output tubing
(ID x OD)
1.14 x 1.57 mm (PE-160)
  1. Na+,K+,2Cl– Cotransport and Intracellular Chloride Regulation in Rat Primary Sensory Neurons: Thermodynamic and Kinetic Aspects
    Rocha-González HI, Mao S, Alvarez-Leefmans FJ
    J Neurophysiol. 2008 Jul;100(1):169-84. Epub 2008 Apr 2
  2. Simultaneous Measurement of Water Volume and pH in Single Cells Using BCECF and Fluorescence Imaging Microscopy
    Francisco J. Alvarez-Leefmans, Jose J. Herrera-Perez, Martin S. Marquez,y and Victor M. Blanco
    Biophysical Journal Volume 90 January 2006 608.618
  3. Modulation of Monocarboxylic Acid Transporter-1 Kinetic Function by the cAMP Signaling Pathway in Rat Brain Endothelial Cells*
    Smith JP, Drewes LR
    J Biol Chem. 2006 Jan 27;281(4):2053-60. Epub 2005 Nov 21
Integrated Components Brochure_06_7_11.pdfIntegrated Components for Live Cell Imaging Brochure
RC-21BRW (080724).pdfRC-21BRW Chamber Instructions
perfusion_strategies.pdfPerfusion Strategies (Leinders-Zufall)