Open Diamond Bath Imaging Chambers (RC-22, RC-22C)

Open Diamond Bath Imaging Chambers (RC-22, RC-22C)

The RC-22 and RC-22C are small volume, open bath recording chambers designed for tissue and brain slice studies.

  • Designed for tissue slice samples or cell cultured coverslips
  • Applications include patch clamp, intracellular / extracellular recordings, and imaging
  • Diamond shaped bath provides laminar solution flow
  • Small bath volume facilitates rapid solution exchange
  • Tissue slice anchor included

Item# Description U.S. List Price Quantity
64-0228 Recording Chamber RC-22 for Tissue Slice with SHD-22L/2 Anchor
64-0229 Recording Chamber RC-22 for Tissue Slice with SHD-22CL/2 Anchor
64-0277 Series 20 Plain Platform, Model P-1
64-0284 Series 20 Heater Platform, Model PH-1
64-1526 PM-1 Chamber platform with magnetic clamps
64-1506 (RPC-1) Replacement platform clamps and screws for P-1, P-3, P-4, P-5, and P-6 platforms, 2 pack
64-1920 (SA-20PS1) Adapter Ring for Pecon Heating Insert P
64-0755 PE-160/10 Polyethylene Tubing PE-160, 10 ft.
64-0756 (PE-160/100) Polyethylene tubing, PE-160, 100 ft
64-0707 CS-22/40 Coverslips, use with RC-22, RC22C, RC-24, RC-24E, RC-24N, RC-26, RC-26G, RC-26GLP, RC-30, and RC-50, pkg of 50
64-0198 Nylon Mesh Kit - 12 pieces
64-0246 Slice Anchor - Stainless Steel for RC-22 Chamber, 1.5 mm, SHD-22L/15
64-0247 Slice Anchor - Stainless Steel for RC-22C Chamber, 1.5 mm, SHD-22CL/15
64-0106 Model CC-28 Cable Assemblies with Connector for TC-324B/344B
64-0107 TA-29 Replacement Cable with Bead Thermistor
64-0274 Heater Elements (2) for PH (Heater) Platforms with Mounting Pads and Screws, RH-2
64-1400 Right Hand Suction Tube, Series 20 Classic Design for use with RC-22/22C, RC-25/25F, RC-26/26G/26GLP/26Z
Login for U.S. Pricing

The RC-22 and RC-22C feature an open bath and include a slice anchor. The diamond shape geometry of these chamber baths facilitate laminar solution flow which can provide minimal distortion of the sample during perfusion.

Slices are secured via a supplied stainless steel anchor (or harp). Threads are made of Lycra© and have a spacing of 1.5 mm. The RC-22 is supplied with an SHD-22L/15 anchor, and the RC-22C is supplied with an SHD-22CL/15 anchor. While anchor mass (typically 300 milligrams) may be sufficient to hold a slice in place, Warner anchors rely on a close fit between the anchor body and the chamber sidewall. This feature can reduce stress on the anchored slice.

Solution aspiration is via a stainless steel suction tube which is secured by a clamp. This insures that the adjustable-height suction tube will not move once set by the user. Each chamber has an agar bridge well for reference electrodes. The working volume of the RC-22 and RC-22C is 115 µl and 138 µl, respectively. Chamber sidewalls have a height of 1.2 mm to facilitate electrode access and placement.

The RC-22 and RC-22C chambers require a platform (PM-1,PH-1 or P-1) and stage adapter to complete the assembly for mounting onto a microscope. The bottom-forming coverslip is 22 x 40 mm.


A temperature controller TC-344C or TC-324C that connects to the heated platform can be added to set and maintain a certain temperature.

Perfusion can be achieved by adding a VC-8 or VC-6 perfusion system, the DVW vacuum system takes care of the waste.

Each chamber is supplied with a slice anchor, a swatch of nylon mesh, a pack of #1 thickness glass coverslips (CS-22/40, 22 x 40 mm, 50/pkg) and 10 ft of PE-160 tubing.

 RC-22 RC-22C
Material polycarbonate polycarbonate
Bath dimension
(L x W x H)
22 x 8 x 3.8 mm 23 x 10 x 3.8 mm
Volume, by depth 115 μl/mm 138 μl/mm
Bottom coverglass 22 x 40 mm 22 x 40 mm
Input tubing
(ID x OD)
1.14 x 1.57 mm (PE-160) 1.14 x 1.57 mm (PE-160)
Aspirator coupling 1.67 mm (OD) 1.67 mm (OD)

RC-22 drawing

  1. Perfusion and Aspiration Techniques
    Warner Instruments
    YouTube 2012

  1. Tuning the excitability of midbrain dopamine neurons by modulating the Ca2+ sensitivity of SK channels
    Ji H, Hougaard C, Herrik KF, Strøbaek D, Christophersen P, Shepard PD
    Eur J Neurosci. 2009 May;29(9):1883-95. Epub 2009 Apr 20
  2. Excitatory synapses in the rat superficial dorsal horn are strengthened following peripheral inflammation during early postnatal development
    Li J, Baccei ML
    Pain. 2009 May;143(1-2):56-64. Epub 2009 Feb 26
  3. Multiple pathological events in exercised dystrophic mdx mice are targeted by pentoxifylline: outcome of a large array of in vivo and ex vivo tests
    Burdi R, Rolland JF, Fraysse B, Litvinova K, Cozzoli A, Giannuzzi V, Liantonio A, Camerino GM, Sblendorio V, Capogrosso RF, Palmieri B, Andreetta F, Confalonieri P, De Benedictis L, Montagnani M, De Luca A
    J Appl Physiol. 2009 Apr;106(4):1311-24. Epub 2009 Jan 8
  4. Cell Electrophysiology with Carbon Nanopipettes
    Schrlau MG, Dun NJ, Bau HH
    ACS Nano. 2009 Mar 24;3(3):563-8
  5. Peripheral nerve injury sensitizes neonatal dorsal horn neurons to tumor necrosis factor
    Li J, Xie W, Zhang JM, Baccei ML
    Mol Pain. 2009 Mar 2;5:10
  6. The Role of L- and T-Type Calcium Channels in Local and Remote Calcium Responses in Rat Mesenteric Terminal Arterioles
    Braunstein TH, Inoue R, Cribbs L, Oike M, Ito Y, Holstein-Rathlou NH, Jensen LJ
    J Vasc Res. 2009;46(2):138-51. Epub 2008 Sep 2
  7. Decreased number of interneurons and increased seizures in neuropilin 2 deficient mice: Implications for autism and epilepsy
    Gant JC, Thibault O, Blalock EM, Yang J, Bachstetter A, Kotick J, Schauwecker PE, Hauser KF, Smith GM, Mervis R, Li Y, Barnes GN
    Epilepsia. 2009 Apr;50(4):629-45. Epub 2008 Jul 24
  8. Oxidative impairment of hippocampal long-term potentiation involves activation of protein phosphatase 2A and is prevented by ketone bodies
    Maalouf M, Rho JM
    J Neurosci Res. 2008 Nov 15;86(15):3322-30
  9. Gentamicin treatment in exercised mdx mice: Identification of dystrophin-sensitive pathways and evaluation of efficacy in work-loaded dystrophic muscle
    De Luca A, Nico B, Rolland JF, Cozzoli A, Burdi R, Mangieri D, Giannuzzi V, Liantonio A, Cippone V, De Bellis M, Nicchia GP, Camerino GM, Frigeri A, Svelto M, Camerino DC
    Neurobiol Dis. 2008 Nov;32(2):243-53. Epub 2008 Jul 23
  10. Developmental Changes in the Fidelity and Short-Term Plasticity of GABAergic Synapses in the Neonatal Rat Dorsal Horn
    Ingram RA, Fitzgerald M, Baccei ML
    J Neurophysiol. 2008 Jun;99(6):3144-50. Epub 2008 Apr 9
  11. Action potential throughput in aged rat hippocampal neurons: Regulation by selective forms of hyperpolarization
    Gant JC, Thibault O
    Neurobiol Aging. 2008 Mar 24
  12. Excitatory effects of human immunodeficiency virus 1 Tat on cultured rat cerebral cortical neurons
    Brailoiu GC, Brailoiu E, Chang JK, Dun NJ
    Neuroscience. 2008 Feb 6;151(3):701-10. Epub 2007 Dec 5
  13. Odorant receptors directly activate phospholipase C/inositol-1,4,5-trisphosphate coupled to calcium influx in Odora cells
    Guang Liu, Robert M. Badeau, Akihiko Tanimura and Barbara R. Talamo
    Journal of Neurochemistry, 2006, 96, 1591–1605
  14. Perinatal hyperoxia for 14 days increases nerve conduction time and the acute unitary response to hypoxia of rat carotid body chemoreceptors
    David F. Donnelly, Insook Kim, Claire Carle and John L. Carroll
    J Appl Physiol 99: 114-119, 2005
Integrated Components Brochure_06_7_11.pdfIntegrated Components for Live Cell Imaging Brochure
RC-22 22C (2000.08.15).pdfRC-22 and RC-22C Chamber Instructions
perfusion_strategies.pdfPerfusion Strategies (Leinders-Zufall)