Open Diamond Bath Imaging Chamber (RC-21BDW)

Open Diamond Bath Imaging Chamber (RC-21BDW)

An open bath, polycarbonate recording chamber that uses 25 mm round coverslips and incorporates Warner's diamond bath fluidics.

  • Designed for physiological measurements on cell cultured coverslips
  • Identical to the RC-21BRW except for a diamond shaped bath
  • Uses A 25 mm round coverslip as the chamber floor
  • Diamond shaped bath provides laminar solution flow
  • Quiet operation
  • Wicked solution removal from bath
  • Can be used with upright or inverted microscopes

Item# Description U.S. List Price Quantity
64-0362 Open Chamber for 25 mm Round Coverslip, 143 ul, RC-21BDW
64-0278 Series 20 Plain Platform, Model P-2
64-0285 Series 20 Heater Platform, Model PH-2
64-1561 PM-2 Chamber platform with magnetic clamps
64-1506 (RPC-1) Replacement platform clamps and screws for P-1, P-3, P-4, P-5, and P-6 platforms, 2 pack
64-0755 PE-160/10 Polyethylene Tubing PE-160, 10 ft.
64-0756 (PE-160/100) Polyethylene tubing, PE-160, 100 ft
64-0705 CS-25R Coverslips, 0.15 mm (0.006 in), 25 mm diameter, pkg of 100
64-0106 Model CC-28 Cable Assemblies with Connector for TC-324B/344B
64-0107 TA-29 Replacement Cable with Bead Thermistor
64-0274 Heater Elements (2) for PH (Heater) Platforms with Mounting Pads and Screws, RH-2
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The low profile design of the RC-21BDW allows good electrode access to the chamber bath. Constant solution level and quiet operation are assured with the use of a wick between the bath and suction well. With laminar flow, the sample is minimally distorted during perfusion. Bath volume is 143 µl for each mm solution height.

RC-21BDW chambers require a platform (PM-2,PH-2 or P-2) and stage adapter to complete the assembly for mounting onto a microscope.

A temperature controller TC-344C or TC-324C that connects to the heated platform can be added to set and maintain a certain temperature.

Perfusion can be achieved by adding a VC-8 or VC-6 perfusion system, the DVW vacuum system takes care of the waste.

Each RC-21BDW chamber is supplied with a package of #1 thickness glass coverslips (CS-25R, 25 mm dia., pkg. of 100) and 10 ft of PE-160 polyethylene tubing.

Material polycarbonate
Bath dimensions
(L x W x H)
17 x 13 x 3.8 mm
Volume, by depth 143 µl/mm
Bottom coverglass 25 mm round
Input tubing
(ID x OD)
1.14 x 1.57 mm (PE-160)
Output tubing
(ID x OD)
1.14 x 1.57 mm (PE-160)

RC-21BDW Drawing

  1. Measuring Near Plasma Membrane and Global Intracellular Calcium Dynamics in Astrocytes
    Shigetomi E, Khakh BS
    J Vis Exp. 2009 Apr 26;(26). pii: 1142. doi: 10.3791/1142
  2. Tissue-specific Regulation of Sodium/Proton Exchanger Isoform 3 Activity in Na+/H+ Exchanger Regulatory Factor 1 (NHERF1) Null Mice
    Murtazina R, Kovbasnjuk O, Zachos NC, Li X, Chen Y, Hubbard A, Hogema BM, Steplock D, Seidler U, Hoque KM, Tse CM, De Jonge HR, Weinman EJ, Donowitz M
    J Biol Chem. 2007 Aug 24;282(34):25141-51. Epub 2007 Jun 19
Integrated Components Brochure_06_7_11.pdfIntegrated Components for Live Cell Imaging Brochure
RC-21BDW (080724).pdfRC-21BDW Chamber Instructions
perfusion_strategies.pdfPerfusion Strategies (Leinders-Zufall)