Open Diamond Bath Imaging Chamber for Oocyte Studies (RC-26Z)

Open Diamond Bath Imaging Chamber for Oocyte Studies (RC-26Z)

The RC-26Z, based on the the popular RC-26, is specifically designed to accommodate studies with xenopus oocytes.

  • Designed for TEV oocyte recording
  • Diamond shaped bath provides laminar solution flow
  • Large optical imaging area
  • Can be used with either upright or inverted microscopes
  • Tissue slice anchors available

Item# Description U.S. List Price Quantity
64-0237 Large Bath Oocyte RC-26Z Recording Chamber
64-0277 Series 20 Plain Platform, Model P-1
64-0284 Series 20 Heater Platform, Model PH-1
64-1526 PM-1 Chamber platform with magnetic clamps
64-1506 (RPC-1) Replacement platform clamps and screws for P-1, P-3, P-4, P-5, and P-6 platforms, 2 pack
64-1920 (SA-20PS1) Adapter Ring for Pecon Heating Insert P
64-0250 Slice Anchor - Stainless Steel for RC-26 Chamber, 1.0 mm, SHD-26H/10
64-0251 Slice Anchor - Stainless Steel for RC-26 Chamber, 1.5 mm, SHD-26H/15
64-0252 Slice Anchor - Stainless Steel for RC-26 Chamber, 2.0 mm, SHD-26H/2
64-0265 Slice Anchor Kit for RC-26 Chamber, SHD-26KIT
64-0755 PE-160/10 Polyethylene Tubing PE-160, 10 ft.
64-0756 (PE-160/100) Polyethylene tubing, PE-160, 100 ft
64-0708 CS-22/50 Coverslips for use with RC-26Z Chambers, 0.15 mm (0.006 in), 22 x 50 mm, pkg of 85
64-0106 Model CC-28 Cable Assemblies with Connector for TC-324B/344B
64-0107 TA-29 Replacement Cable with Bead Thermistor
64-0274 Heater Elements (2) for PH (Heater) Platforms with Mounting Pads and Screws, RH-2
64-1400 Right Hand Suction Tube, Series 20 Classic Design for use with RC-22/22C, RC-25/25F, RC-26/26G/26GLP/26Z
64-1429 Suction Tube Upgrade Kit for Series 20 Chambers
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The RC-26Z is a polycarbonate chamber, similar in design to the RC-26, having two additional features specific for two-electrode oocyte recording: namely, the dam height in the suction well is increased to 2.5 mm to provide an adequate solution level in the bath, and a second agar bridge well has been added to accommodate a bath clamp electrode.

The RC-26Z can also accommodate larger tissue slices and preparations, and permits excellent access for electrodes. The working volume is 170 µl/mm. The chamber floor consists of a 22 x 50 mm coverslip.

The chamber is equipped with a stainless steel suction tube (1.67 mm OD) together with a suction tube clamp. This insures the adjustable suction tube will not move once the height is set by the user.

RC-26Z chamber requires a platform (PM-1, PH-1, or P-1) and stage adapter to complete the assembly for mounting onto a microscope.

A temperature controller TC-344C or TC-324C that connects to the heated platform can be added to set and maintain a certain temperature.

Perfusion can be achieved by adding a VC-8 or VC-6 perfusion system, the DVW vacuum system takes care of the waste.

Each RC-26Z is supplied with a package of #1 thickness glass coverslips (CS-22/50, 22x50 mm, 50/pkg) and 10 ft of PE-160 tubing.

Material Polycarbonate
Bath dimensions
(L x W x H)
25 x 12 x 4 mm
Volume, by depth 170 μl/mm
Bottom coverglass 22 x 50 mm
Input tubing
(ID x OD)
1.14 x 1.57 mm (PE-160)
Aspirator coupling 1.67 mm (OD)

 RC-26Z drawing

  1. Anxiolytic Effects of Maxipost (BMS-204352) and Retigabine via Activation of Neuronal Kv7 Channels ,
    M. P. G. Korsgaard, B. P. Hartz, W. D. Brown, P. K. Ahring, D. Strøbæk, and N. R. Mirza,
    JPET 314:282-292, 2005
Integrated Components Brochure_06_7_11.pdfIntegrated Components for Live Cell Imaging Brochure
RC-26Z (080724).pdfRC-27Z Chamber Instructions
perfusion_strategies.pdfPerfusion Strategies (Leinders-Zufall)