Microincubator (CSMI)

Microincubator (CSMI)

The CSMI is a versatile microscope-stage incubator for cell/tissue culture work using chambered glass slides.

  • Ideal for rectangular chambered slides and chambered coverglass
  • Provides comprehensive micro-environment control for pH, temperature, perfusion and atmosphere
  • Uses Peltier technology
  • Heats and cools from ~5° to 50°C
  • Accommodates up to 4 pre-temperature regulated perfusion lines
  • Provides temperature regulated gas superfusion
  • Magnetic base for easy setup of different configurations
  • Uses a unique aspirator for perfusate removal with minimal disturbance of fluid level

Item# Description U.S. List Price Quantity
65-0101 CSMI Chambered Slide Micro-Incubator
64-0103 SF-28 Slow Flow In-Line Solution Heater
65-0057 Model BSC-T3 Thermistor Bath for use with PDMI-2, PSMI, LU-CB1, and TC-202A (36 KW total)
65-0052 Mini Magnets for Top Surface of Micro-Incubators (LU-CB-1, PDMI-2, or PSMI), set of 4
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The CSMI is a versatile microscope stage incubator designed for cell/tissue culture work. It easily accommodates the Lab-Tek® series of disposable, rectangular chamber slides and chambered coverglasses from Nunc. It also supports the Falcon® CultureSlide series from Beckton Dickinson.

The CSMI, in combination with a matching low noise TC-202A temperature controller, facilitates the long term maintenance of tissue or cell cultures on a microscope stage. This allows optical monitoring of dyes, micro-injection, electrical recording, and micro-manipulation for many hours or even days. The CSMI not only facilitates the precise regulation and manipulation of bath temperature, but it also supports multi-channel perfusion (up to 4 channels) and atmosphere maintenance (e.g., CO2 superfusion for pH control).

The CSMI is based on a successful design of the Medical Systems PDMI-2 microincubator platform for 35 mm Petri dishes. The CSMI utilizes a Peltier thermoelectric device to regulate temperature over a wide range, both above and below ambient levels.

The CSMI easily fits on the stage of inverted microscopes from major microscope manufacturers including Zeiss, Nikon, Olympus and Leica.

Temperature Range ~5° to 10°C below ambient to 50°C
Accommodated Disposable Chambers
  1. Nalge Nunc chambered slides and cover slips
  2. Becton Dickinson Falcon chambered slides
Accommodated Microscopes
  1. Zeiss Axiovert with attachable mechanical stage
  2. Leica DAS Microscope DMIL and DMIRB/E with attached mechanical stage
  3. Nikon Diaphot
  4. Olympus IX50/70 or IMT-2 fixed stage
Peltier TED Current Rating 6 Amps at DC max
Built In Plate Thermistor 100 kΩ at 25°C
Perfusion Inlet Lines 26 GA PTFE capillary up to 4 lines can be installed simultaneously
Perfusion Rate 3 ml/min nominal total
Perfusate Outlet LU-ASP aspirator
Gas Port 1/16 in. barb
Gas Superfusion Flow Rate 0.5 to 2.0 L/min
Weight 17.9 oz (0.5 kg)
Overall Dimensions 6.5 x 4 x 1 in (16.5 x 10.2 x 2.5 cm)