NEW! Solutions for Microdialysis of Large Molecules

NEW! Solutions for Microdialysis of Large Molecules

NEW! SOLUTIONS FOR MICRODIALYSIS OF LARGE MOLECULES

CMA offers new complete solutions for the study of large molecules using the microdialysis techniques.

  • New Microdialysis Probes for Large Molecules
  • New CNS Perfusion Fluid with Dextran MW 500 kDa 3%
  • New Push-Pull Set-ups

Item# Description U.S. List Price Quantity
CMA8012411 CMA 7 55 kDa Microdialysis Probe, 1 mm membrane length, pkg. of 3
CMA8012412 CMA 7 55 kDa Microdialysis Probe, 2 mm membrane length, pkg. of 3
CMA8012421 CMA 7 500 kDa Microdialysis Probe, 1 mm membrane length, pkg. of 3
CMA8012422 CMA 7 500 kDa Microdialysis Probe, 2 mm membrane length, pkg. of 3
CMA8012423 CMA 7 2 MDa Microdialysis Probe, 1 mm membrane length, pkg. of 3
CMA8012424 CMA 7 2 MDa Microdialysis Probe, 2 mm membrane length, pkg. of 3
CMA8012301 CMA 8 High Cut-Off Microdialysis Probe, 1 mm membrane length (pkg. of 3)
CMA8012302 CMA 8 High Cut-Off Microdialysis Probe, 2 mm membrane length (pkg. of 3)
CMA8012511 CMA 11 55 kDa Microdialysis Probe, 1 mm membrane length, pkg. of 3
CMA8012512 CMA 11 55 kDa Microdialysis Probe, 2 mm membrane length, pkg. of 3
CMA8012513 CMA 11 55 kDa Microdialysis Probe, 3 mm membrane length, pkg. of 3
CMA8012514 CMA 11 55 kDa Microdialysis Probe, 4 mm membrane length, pkg. of 3
CMA8012521 CMA 11 500 kDa Microdialysis Probe, 1 mm membrane length, pkg. of 3
CMA8309661 CMA 12 HighCO Probe - 1 mm (pkg. of 3)
CMA8309662 CMA 12 HighCO Probe - 2 mm (pkg. of 3)
CMA8309663 CMA 12 HighCO Probe - 3 mm (pkg. of 3)
CMA8309664 CMA 12 HighCO Probe - 4 mm (pkg. of 3)
75-0512 REGLO ICC Digital 2-Channel, 12-Roller Peristaltic Pump, 100 to 240 VAC, 50/60 Hz
CMA8050151 Perfusion Fluid CNS Dextran MW 500 kDa 3%, 5 ml, pkg. of 2
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NEW! SOLUTIONS FOR MICRODIALYSIS OF LARGE MOLECULES

CMA offers complete solutions for the study of large molecules using the microdialysis techniques.

Why large molecules in microdialysis research?

The use of the microdialysis technique for the recovery of large molecules is of increasing interest as it offers the opportunity to identify patterns of protein expression in a variety of tissue spaces as well as to evaluate useful biomarkers of disease.

From this perspective, the use of this technique contributes to a better understanding of the disease process and its diagnosis, allowing the development of more targeted approaches to therapy.

The challenges of the microdialysis of large molecules

The use of probes with large pore size membranes required for the microdialysis of large molecules creates some methodological challenges such as protein aggregation, protein adhesion, ultrafiltration and low fluid recovery/extraction efficiency. All these challenges can now be overcome by using appropriate and optimized microdialysis set-ups.

New probes for large molecules!

CMA offers a set of microdialysis probes for the recovery of large molecules (such as proteins, cytokines, growth factors, antibody and neuropeptides…) on awake rodents.

NEW! CNS Probes for Mice

  • CMA 7, 55 kDa, 500 kDa, 2 MDa cut-off
  • CMA 8, 100 kDa cut-off

NEW! CNS Probes for Rats

The probes/guides are available as metal free, beta-irradiated (sterile) and custom-made (shaft/membrane length) on demand.

How to avoid Ultra-Filtration and improve Extraction Efficiency?

New! Dextran solution for 55 to 100 kDa High Cut-off Microdialysis Probes

We recommend the use of the CMA Microdialysis CNS Perfusion fluid with Dextran 500 kDa 3% in order to stabilize the pressure within the probe and limit ultra-filtration.

The CNS Perfusion Fluid with Dextran 500 kDa 3% is suitable for our CNS high cut-off probes with 55 and 100 kDa cut-off for rats and mice. The Dextran 500 kDa will not diffuse out of the probe and cause additional tissue inflammation. It is reported the Dextran 500 can give fluid recovery close to 100%.

The solution is sterile, ready to use and non-buffered (so pH would not change).

  • See also the Dextran 500 kDa 3% Product Note for more details

New! Push-pull set-up for 500 kDa to 2 MDa Ultra-High Cut-off Microdialysis Probes

The Dextran 500 kDa 3% solution cannot be used for the 500 kDa to 2 MDa Ultra-High Cut-off microdialysis probes because of the large pore size membranes that would let the Dextran pass into the extracellular space and cause inflammation.

One way to avoid ultra-filtration with very high cut-off membranes is to reduce the back pressure caused by the perfusion fluid coming from the probe inlet.

From this perspective, we recommend using the REGLO ICC Peristaltic Pump for Ultra-High Cut-off Probes. We have tested this pump to ensure that the minimum flow rate of the pump (0.1 μl/min) is accurate and that the 12 rollers provide a smooth flow. Flow rate and direction can be individually adjusted for each channel. The pump calibration is easy for the user. A ready to use Peristaltic Tubing Kit is available for fast connection to microdialysis FEP tubing.

SpecificationsCMA8012411CMA8012412CMA8012421CMA8012422CMA8012423CMA8012424CMA8012301CMA8012302CMA8012511CMA8012512CMA8012513CMA8012514CMA8012521CMA8309661CMA8309662CMA8309663CMA8309664
Membrane Length1 mm2 mm1 mm2 mm1 mm2 mmCMA 8 High Cut-off Microdialysis probeCMA 8 High Cut-off Microdialysis probe1 mm2 mm3 mm4 mm1 mm1 mm2 mm3 mm4 mm
Membrane O D0.26 mm0.26 mm0.28 mm0.28 mm0.28 mm0.28 mmPES, 100kDAPES, 100kDA0.26 mm0.26 mm0.26 mm0.26 mm0.28 mm0.5 mm0.5 mm0.5 mm0.5 mm
Membrane MaterialPolyestersulfone (PES)Polyestersulfone (PES)Polyestersulfone (PES)Polyestersulfone (PES)Polyestersulfone (PES)Polyestersulfone (PES)1 mm2 mmPolyestersulfone (PES)Polyestersulfone (PES)Polyestersulfone (PES)Polyestersulfone (PES)Polyestersulfone (PES)PESPESPESPES
Molecular Weight Cut-off55 kD55 kD500 kD500 kD2 MDa2 MDa0.5 mm0.5 mm55 kD55 kD55 kD55 kD500 kD100 kDA MWCO100 kDA MWCO100 kDA MWCO100 kDA MWCO
Shaft Diameter0.40 mm0.40 mm0.40 mm0.40 mm0.40 mm0.40 mm0.4 mm0.4 mm0.40 mm0.40 mm0.40 mm0.40 mm0.40 mm0.64 mm0.64 mm0.64 mm0.64 mm
Shaft Length Metric7 mm7 mm14 mm14 mm14 mm14 mm7 mm7 mm14 mm14 mm14 mm14 mm14 mm14 mm14 mm14 mm14 mm
Intlet Internal Volume0.06 µL0.06 µL0.06 µL0.06 µL0.06 µL0.06 µL negligible negligible0.1 µL0.1 µL0.1 µL0.1 µL0.1 µLnegligiblenegligiblenegligiblenegligible
Outlet Internal Volume0.3 µL0.3 µL0.3 µL0.3 µL0.3 µL0.3 µL3 μL3 μL1 µL1 µL1 µL1 µL1 µL0.3 µ0.3 µ0.3 µ0.3 µ
Model3.6 µL3.6 µL3.5 µL3.5 µL3.5 µL3.5 µLStandardStandardStandardStandardStandardStandardStandardStandardStandard