Membrane-Bottom Filter Plates

Membrane-bottom filter plates

48-well and 96-well filter plates bring speed and high throughput to sample filtration on a microliter to milliliter scale. The membrane-bottom individual sample wells or chambers have separate high-strength single or dual filter membranes to provide rapid filtration rates and to eliminate leakage or cross-talk between adjacent wells.

Key to membrane types:

PE = polyethylene

UHMWPE = ultra-high molecular weight polyethylene

PP = polypropylene

PES = polyethersulfone

PVDF = polyvinylidene difluoride 

GF = glass fiber

Item# Description U.S. List Price Quantity
74-5551 96-well Membrane-Bottom Filter Plate, Ultra Filtration, PES, 10 kDa MWCO, pkg. of 5
74-5552 96-well Membrane-Bottom Filter Plate, Ultra Filtration, PES, 30 kDa MWCO, pkg. of 5
74-5553 96-well Membrane-Bottom Filter Plate, 300 µl, GF 1.2 µm, short drip, pkg. of 10
74-5554 96-well Membrane-Bottom Filter Plate, 300 µl, Hydrophilic PVDF 0.45 µm, short drip, pkg. of 10
74-5555 96-well Membrane-Bottom Filter Plate, 400 µl, Hydrophilic PVDF 0.45 µm, long drip, pkg. of 5
74-5556 96-well Membrane-Bottom Filter Plate, 800 µl, Hydrophilic PVDF 0.45 µm, long drip, pkg. of 5
74-5557 96-well Membrane-Bottom Filter Plate, 2 ml, PP 0.45 µm, long drip, pkg. of 5
74-5558 96-well Membrane-Bottom Filter Plate, 2 ml, UHMWPE 25 µm, long drip, pkg. of 5
74-5585 96-well Membrane-Bottom Filter Plate, 400 µl, PES, 100 kDa MWCO, short drip. Pkg. of 5
74-5586 96-well Membrane-Bottom Filter Plate, 400 µl, PES, 300 kDa MWCO, short drip. Pkg. of 5
74-5559 48-well Membrane-Bottom Filter Plate, 5 ml, PE frit 25 µm, long drip, pkg. of 5
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48-well and 96-well membrane-bottom filter plates bring speed and high throughput to sample filtration on a microliter to milliliter scale. The membrane-bottom individual sample wells or chambers have separate high-strength single or dual filter membranes to provide rapid filtration rates and to eliminate leakage or cross-talk between adjacent wells.

  • High recoveries of both filtrates and particulate retentates
  • Multi-well format for simultaneous filtration of 96 or 384 different samples
  • Individual filter membranes to avoid cross-talk between adjacent wells
  • High well-to-well reproducibility
  • Membrane pore sizes of 0.45 to 25 µm (other pore sizes available on request)
  • Suitable for vacuum manifold filtration or centrifugal filtration

All filter plates feature rigid polypropylene construction for chemical resistance and low binding and meet SBS footprint for use in robotic systems. Standard filter media include: glass fiber, PVDF polypropylene, polyethylene and polyether sulfone. Patent pending sealing process guarantees no well-to-well cross talk and weeping and allows superior recovery performance. 

Applications:

  • Nucleic Acid Binding
  • DNA Binding
  • DNA/RNA Purification
  • Purification of PCR Products
  • High-Throughput Preparation of YAC DNA
  • High-Throughput Synthesis of Drugs
  • Bead/Resin Based Assays
  • Cell-Based Receptor Binding Assays
  • Size exclusion
  • Concentrate, purify and desalt proteins, peptides, oligos, DNA and RNA
  • Recover proteins, oligos and RNA from polyacrylamide gels
  • Filtration and Filtrate Collection 

96-well Ultra-Filter Plates with 6.24 mm well size and 19.35 sq mm filter area are good for size exclusion, to concentrate, purify and desalt proteins, peptides, oligos, DNA and RNA and also to recover proteins, oligos and RNA from polyacrylamide gels. Plates comply with industry standard for automation and are available with 10,000 and 30,000 MWCO membranes. 

96-well 2 ml Filter Plates with 8.23 mm well size come with polypropylene membrane for organic synthesis and with UHMW polyethylene membrane good for solid phase extraction.

Distinctively constructed 384-well Filter Plates have short drip directors, square to round wells for maximum working volumes and variety of filtration media and can also be used robotically as well as for vacuum or centrifuge filtrations.

Application specific Filter Plates and Filtration Devices to the Biopharmaceutical industry can be custom ordered. Other package sizes and bar-coding are available.

 

Specifications
Item #  Filter Area, mm2  Filter Media Frit Size, µm  MWCO, kDa  Sample Size, µl  Well Size, mm 
 74-5551  19.35 PES    10    6.25
 74-5552  19.35 PES    30    6.25
 74-5553   GF  1.2    300  8.23
 74-5554   Hydrophilic PVDF  0.45    300  8.23
 74-5555   Hydrophilic PVDF  0.45    400  8.23
 74-5556   Hydrophilic PVDF  0.45    800  8.23
 74-5557   PP  0.45    2000  8.23
 74-5558   UHMWPE  25    2000  8.23
 74-5585   PES    100    
 74-5586   PES    300    
 74-5559   PE  25    5000