Marsh Ganglion Bath
Intended to test the action of drugs on the synaptic transmission or nerve conduction in the vagus nerve or the cervical ganglion
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The Marsh Ganglion Bath is intended to test the action of drugs on the synaptic transmission or nerve conduction of the vagus nerve or the cervical ganglion.
- Synaptic transmission studies of vagus nerve or cervical ganglion
- Nerve conduction studies of vagus nerve or cervical ganglion
The bath is an open-top Perspex bath with inflow and outflow connections. It is divided into three compartments by two separators. Each of these separators consists of a removable upper section and a lower section with a cutout to allow the nerve to pass between chambers without crushing and also support the tissue in position.
The first chamber contains holders for two platinum electrodes for axonal stimulation of the nerve bundle or preganglionic nerve trunk. Recordings are made from the central chamber with the third chamber acting as a reference chamber using non-polarizing silver/silver chloride electrodes. Drugs are introduced via the continously perfused central chamber.
The optional electrode set to the Marsh Ganglion Bath includes the necessary components to produce two recording electrodes to interconnect the recording chamber with the PHDA headstage amplifier.
The electrode set contains two 1 ml syringes, 2 lengths of PE tubing with Luer tip, 2 Ag/AgCl pellets with taper plastic body and connecting socket at the back suitable for 2 mm plug pin and Agar powder. To be assembled for use. (See image.)
If stored properly (protected from light sources), electrodes can be used for about two weeks. Th Agar solution must then be replaced. The electrodes are connected by short wires with mm pins to the headstage of the amplifier. These cables are supplied with the PLUGSYS PHDA amplifier.
Perspex bath, platin stimulation electrodes (includes two simulation electrodes with cables. Can be ordered separately (73-3425) for spare parts.)
Amplifier with high-impedance input and a peak height detector (PLUGSYS module PHDA), data acquisition, electrical stimulator with isolation unit, thermocirculator, solution reservoir, illumination, oscilloscope.