Marsh Ganglion Bath

Marsh Ganglion Bath

Intended to test the action of drugs on the synaptic transmission or nerve conduction in the vagus nerve or the cervical ganglion

To ensure that your system is properly configured as a functional unit that meets your application needs, please complete our Checklist and then contact Technical Services at 800-547-6766 or via email at support@hbiosci.com before placing an order. In Europe, please call +49 7665 92000 or via email at sales@hugo-sachs.de.

Item# Description U.S. List Price Quantity
73-2414 Marsh Ganglion Bath
73-0387 Electrodes Component Set for Marsh Ganglion Bath for 2 Electrodes (Option, not included)
73-3425 Platin Stimulation Electrodes with Cables for Marsh Ganglion Bath (Replacement Parts)
Login for U.S. Pricing


The Marsh Ganglion Bath is intended to test the action of drugs on the synaptic transmission or nerve conduction of the vagus nerve or the cervical ganglion.

Applications
Set Up & Operation
Electrodes Component Set
Included Items
Additional Equipment Required

Applications

  • Synaptic transmission studies of vagus nerve or cervical ganglion
  • Nerve conduction studies of vagus nerve or cervical ganglion

Set Up & Operation

 The bath is an open-top Perspex bath with inflow and outflow connections. It is divided into three compartments by two separators. Each of these separators consists of a removable upper section and a lower section with a cutout to allow the nerve to pass between chambers without crushing and also support the tissue in position.

The first chamber contains holders for two platinum electrodes for axonal stimulation of the nerve bundle or preganglionic nerve trunk. Recordings are made from the central chamber with the third chamber acting as a reference chamber using non-polarizing silver/silver chloride electrodes. Drugs are introduced via the continously perfused central chamber.


Electrodes Component Set

The optional electrode set to the Marsh Ganglion Bath includes the necessary components to produce two recording electrodes to interconnect the recording chamber with the PHDA headstage amplifier.
Order separately.

The electrode set contains two 1 ml syringes, 2 lengths of PE tubing with Luer tip, 2 Ag/AgCl pellets with taper plastic body and connecting socket at the back suitable for 2 mm plug pin and Agar powder. To be assembled for use. (See image.)

If stored properly (protected from light sources), electrodes can be used for about two weeks. Th Agar solution must then be replaced. The electrodes are connected by short wires with mm pins to the headstage of the amplifier. These cables are supplied with the PLUGSYS PHDA amplifier.


Included Items

 Perspex bath, platin stimulation electrodes (includes two simulation electrodes with cables. Can be ordered separately (73-3425) for spare parts.)

Additional Equipment Required

 Amplifier with high-impedance input and a peak height detector (PLUGSYS module PHDA), data acquisition, electrical stimulator with isolation unit, thermocirculator, solution reservoir, illumination, oscilloscope.

Specifications