Low Profile Parallel Plate Flow Chamber (RC-31)
The RC-31 is a closed bath, low profile chamber incorporating special features for microscopic confocal imaging.
- Adjustable distance between upper and lower coverslips, 50 µm to 350 µm
- Optimal control of culture conditions and cellular environment
- Compatible with both upright and inverted microscopes
- Amenable for long term studies using live cells
- Uses Warner's Series 30 stage adapters
Unique features of the RC-31 include user defined bath geometry and a large viewing area. A 30 mm, #1.5 glass coverslip forms the top of the chamber, while a 40 mm, #1.5 coverslip forms the bottom, respectively, creating a closed bath. The closed design allows the chamber to be inverted in the mounting platform enabling its use with both inverted or upright microscopes. The final viewing aperture is 25 mm diameter.
Variable Bath Volume/Fast Perfusate ExchangeThe side walls of the RC-31 bath is formed by a silicone gasket sandwiched between the top and bottom coverslips. Wall height (i.e. spacing between the two coverslips) can range from 50-350 µm and is defined by the thickness and number of gaskets used.
GasketsPre-cut gaskets having two different geometries are also supplied; one having a wide cut to allow full use of the 17.7 mm diameter aperture window, and a narrow, slotted version (4 x 37 mm) designed for fast solution exchange. Blank gaskets are also supplied with the chamber permitting the development of the customized bath geometry required for individual applications.
The RC-31 comes complete with 30 mm round top coverglass, 40 mm round bottom coverglass, blank gaskets (10 pk each in 150, 250, and 375 µm thickness), slotted gaskets (10 pk each in 150, 250, and 375 µm thickness), and wide gaskets (10 pk each in 150, 250, and 375 µm thickness), and 10 ft PE-90 Tubing
Specifications |
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Physical Dimensions (H X D) | 11.5 x 82 mm | ||||
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Viewing Area | 23 mm in diameter | ||||
Bath volumes | For 250um thick gaskets:
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Weight | 0.22 kg, assembled with optional heaters | ||||
Construction | Aluminum, black anodized | ||||
Gaskets | Silicone rubber | ||||
Input/Output Tubing | PE-90 | ||||
Coverslips | Top: 30 mm round, #1.5 Thickness Bottom: 40 mm round, #1.5 Thickness |
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Microscope Stage Adapters* | Uses Series 30 All major models: Leica, Nikon, Zeiss, Olympus, Prior, Ludl, Burleigh |
DOCUMENT | DESCRIPTION |
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Integrated Components Brochure_06_7_11.pdf | Integrated Components for Live Cell Imaging Brochure |