Low Profile Open Diamond Bath Imaging Chamber (RC-26GLP)

Low Profile Open Diamond Bath Imaging Chamber (RC-26GLP)

Primarily designed for studies of cell cultured coverslips.

  • Applications include patch clamp, intracellular/extracellular recordings, and imaging
  • Extra-low profile facilitates electrode access
  • Diamond shaped bath provides laminar solution flow
  • Broad optical imaging area
  • Can be used with either upright or inverted microscopes

Item# Description U.S. List Price Quantity
64-0236 Low Profile Large Bath RC-26GLP Recording Chamber
64-0277 Series 20 Plain Platform, Model P-1
64-0284 Series 20 Heater Platform, Model PH-1
64-1526 PM-1 Chamber platform with magnetic clamps
64-1506 (RPC-1) Replacement platform clamps and screws for P-1, P-3, P-4, P-5, and P-6 platforms, 2 pack
64-1920 (SA-20PS1) Adapter Ring for Pecon Heating Insert P
64-0253 Slice Anchor - Stainless Steel for RC-26G Chamber, 1.0 mm, SHD-26GH/10
64-0254 Slice Anchor - Stainless Steel for RC-26G Chamber, 1.5 mm, SHD-26GH/15
64-0255 Slice Anchor - Stainless Steel for RC-26G Chamber, 2.0 mm, SHD-26GH/2
64-0755 PE-160/10 Polyethylene Tubing PE-160, 10 ft.
64-0756 (PE-160/100) Polyethylene tubing, PE-160, 100 ft
64-0707 CS-22/40 Coverslips, use with RC-22, RC22C, RC-24, RC-24E, RC-24N, RC-26, RC-26G, RC-26GLP, RC-30, and RC-50, pkg of 50
64-0106 Model CC-28 Cable Assemblies with Connector for TC-324B/344B
64-0107 TA-29 Replacement Cable with Bead Thermistor
64-0274 Heater Elements (2) for PH (Heater) Platforms with Mounting Pads and Screws, RH-2
64-1400 Right Hand Suction Tube, Series 20 Classic Design for use with RC-22/22C, RC-25/25F, RC-26/26G/26GLP/26Z
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RC-26GLP shown in a P-1 chamber platform
RC-26GLP shown in a PM-1 platform

The RC-26GLP is a low profile version of the RC-26G recording chamber. The sides of this chamber have been milled to a height of only 2 mm and the lower profile provides extra clearance for upright microscopes, water immersion objectives, and permits good electrode access to the chamber bath.

The chamber floor is comprised of a 22 x 40 mm coverslip. The working volume is 234 µl per 1.0 mm of solution height. The chamber is equipped with a stainless steel suction tube together with clamp. The clamp insures the adjustable aspirator tube will not move once the height is set by the user.

The RC-26GLP chamber requires a platform (PM-1, PH-1, or P-1) and stage adapter to complete the assembly for mounting onto a microscope.

A temperature controller TC-344C or TC-324C that connects to the heated platform can be added to set and maintain a certain temperature.

Perfusion can be achieved by adding a VC-8 or VC-6 perfusion system, the DVW vacuum system takes care of the waste.

Each RC-26GLP chamber is supplied with a package of CS-22/40 glass coverslips (#1 thickness, 22 x 40 mm, 100/pkg) and 10 ft of PE-160 tubing.

Material polycarbonate
Bath dimensions
(L x W x H)
24 x 15 x 2 mm
Volume, by depth 234 μl/mm
Bottom coverglass 22 x 40 mm
Input tubing
(ID x OD)
1.14 x 1.57 mm (PE-160)
Aspirator coupling 1.67 mm (OD)

RC-26GLP Drawing

  1. Activation of {delta}-Opioid Receptors Reduces Excitatory Input to Putative Gustatory Cells Within the Nucleus of the Solitary Tract
    Zhu M, Cho YK, Li CS
    J Neurophysiol. 2009 Jan;101(1):258-68
  2. Ketamine, But Not Phencyclidine, Selectively Modulates Cerebellar GABAA Receptors Containing {alpha}6 and {delta} Subunits
    Hevers W, Hadley SH, Lüddens H, Amin J
    J Neurosci. 2008 May 14;28(20):5383-93
  3. Preserved BK channel function in vasospastic myocytes from a dog model of subarachnoid hemorrhage
    Jahromi BS, Aihara Y, Ai J, Zhang ZD, Weyer G, Nikitina E, Yassari R, Houamed KM, Macdonald RL
    J Vasc Res. 2008;45(5):402-15. Epub 2008 Apr 10
  4. Synaptogenesis of the Calyx of Held: Rapid Onset of Function and One-to-One Morphological Innervation
    Hoffpauir BK, Grimes JL, Mathers PH, Spirou GA
    J Neurosci. 2006 May 17;26(20):5511-23
  5. Enhancement of long-term memory retention and short-term synaptic plasticity in cbl-b null mice
    Tan DP, Liu QY, Koshiya N, Gu H, Alkon D
    Proc Natl Acad Sci U S A. 2006 Mar 28;103(13):5125-30. Epub 2006 Mar 20
  6. Induction of photosensitivity by heterologous expression of melanopsin
    Qiu X, Kumbalasiri T, Carlson SM, Wong KY, Krishna V, Provencio I, Berson DM
    Nature. 2005 Feb 17;433(7027):745-9
  7. In vivo imaging of C. elegans ASH neurons: cellular response and adaptation to chemical repellents
    Hilliard MA, Apicella AJ, Kerr R, Suzuki H, Bazzicalupo P, Schafer WR
    EMBO J. 2005 Jan 12;24(1):63-72
  8. Layer-specific Production of Nitric Oxide during Cortical Circuit Formation in Postnatal Mouse Brain
    Taiko Imura, Shigeaki Kanatani, Satoshi Fukuda, Yusei Miyamoto and Tatsuhiro Hisatsune
    Cerebral Cortex 2005 15(3):332-340
  9. Two Distinct Subpopulations of Nestin-Positive Cells in Adult Mouse Dentate Gyrus
    Fukuda S, Kato F, Tozuka Y, Yamaguchi M, Miyamoto Y, Hisatsune T
    J Neurosci. 2003 Oct 15;23(28):9357-66
  10. Phototransduction by Retinal Ganglion Cells That Set the Circadian Clock
    Berson DM, Dunn FA, Takao M
    Science. 2002 Feb 8;295(5557):1070-3
  11. Melanopsin-Containing Retinal Ganglion Cells: Architecture, Projections, and Intrinsic Photosensitivity
    Hattar S, Liao HW, Takao M, Berson DM, Yau KW
    Science. 2002 Feb 8;295(5557):1065-70
Integrated Components Brochure_06_7_11.pdfIntegrated Components for Live Cell Imaging Brochure
RC-26GLP (080724).pdfRC-26GLP Chamber Instructions
perfusion_strategies.pdfPerfusion Strategies (Leinders-Zufall)