Large Bath Chamber with Slice Supports (RC-27L)

Large Bath Chamber with Slice Supports (RC-27L)

A large rectangular bath with a slice support and anchor increases slice viability during long term studies.

  • Designed for tissue slice studies
  • Permits solution flow both above and below a tissue slice
  • Provides good access for immersion optics and electrodes.
  • Can be used with either upright or inverted microscopes
  • Slice anchors available

Item# Description U.S. List Price Quantity
64-0241 Large Bath RC-27L Recording Chamber
64-0283 Series 20 Plain Platform, Model P-6D
64-0290 Series 20 Heater Platform, Model PH-6D
64-1528 PM-6D Chamber platform with magnetic clamps
64-1506 (RPC-1) Replacement platform clamps and screws for P-1, P-3, P-4, P-5, and P-6 platforms, 2 pack
64-0190 Plastic Slice Anchor Kit for RC-27L and RC-29 Chambers, SHD-27LPKIT
64-0259 Slice Anchor - Plastic for RC-27L & RC-29, 1.0 mm, SHD-27LP/10
64-0260 Slice Anchor - Plastic for RC-27L & RC-29, 1.5 mm, SHD-27LP/15
64-0261 Slice Anchor - Plastic for RC-27L & RC-29, 2.0 mm, SHD-27LP/2
64-0256 Slice Anchor - Stainless Steel for RC-27L & RC-29, 1.0 mm, SHD-27LH/10
64-0257 Slice Anchor - Stainless Steel for RC-27L & RC-29, 1.5 mm, SHD-27LH/15
64-0258 Slice Anchor - Stainless Steel for RC-27L & RC-29, 2.0 mm, SHD-27LH/2
64-0268 Slice Anchor Kit for RC-27L and RC-29 Chambers, SHD-27LKIT
64-0709 CS-24/50 Coverslips for use with RC-27, RC-27L, RC-27N, RC-27NE, RC-28, and RC-29, 0.15 mm (0.006 in)24 x 50 mm, pkg of 65
64-0755 PE-160/10 Polyethylene Tubing PE-160, 10 ft.
64-0756 (PE-160/100) Polyethylene tubing, PE-160, 100 ft
64-0273 Slice Support for RC-27L and RC-29, SS-3
64-1533 (SS-3G) Mesh slice support for RC-27L, RC-29 recording chambers
64-0106 Model CC-28 Cable Assemblies with Connector for TC-324B/344B
64-0107 TA-29 Replacement Cable with Bead Thermistor
64-0274 Heater Elements (2) for PH (Heater) Platforms with Mounting Pads and Screws, RH-2
64-1401 Left Hand Suction Tube, Series 20 Classic Design for use with RC-27, RC-27L, RC-27NE
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The RC-27L is designed with tissue slice studies in mind. The tissue slice rests on a slice support and is held in place by a slice anchor (also known as a harp). This design allows the tissue slice to be perfused from both above and below, which can increase the sample.s viability. The slice support rests 0.5 mm above the coverslip floor and are included with the RC-27L.

Slice anchors are sold separately.

The slice support frame is manufactured in polycarbonate and uses fine Lycra threads to suspend the slice. Thread spacing on the slice support is 1 mm.

Slice anchors are available in polycarbonate or stainless steel, and thread spacing on anchors are 1.0, 1.5, and 2.0 mm which allows for maximum access to the preparation. In addition to individual anchors, Warner offers polycarbonate or stainless steel slice anchor kits containing anchors in all available thread spacing.

While the weight of a metal harp is sufficient to secure a slice into place, the plastic harp relies on a close apposition between itself and the chamber side walls to maintain its position. This approach can reduce pressure on the slice from the harp.

The RC-27L requires a platform ( (PM-6D, PH-6D, or P-6D) and stage adapter to complete the assembly for mounting onto a microscope.

A temperature controller TC-344C or TC-324C that connects to the heated platform can be added to set and maintain a certain temperature.

Perfusion can be achieved by adding a VC-8 or VC-6 perfusion system, the DVW vacuum system takes care of the waste.

Each chamber is supplied with an SS-3 slice support, 10 ft of PE-160 polyethylene tubing, and a package of #1 thickness glass coverslips (CS-24/50, 24 x 50 mm, pkg. of 50).

Material Polycarbonate
Bath dimensions
(L x W x H)
38 x 18 x 3 mm
Outer dimensions
(L x W x H)
50 x 25 x 4 - 8 mm
Volume, by depth 560 μl/mm
Bottom coverglass 24 x 50 mm
Slice support 24 x 50 mm, Polycarbonate
Lycra threads at 1 mm
Input tubing
(ID x OD)
1.14 x 1.57 mm (PE-160)
Aspirator coupling 1.67 mm (OD)

RC-27L Drawing

  1. Novel mGluR- and CB1R-Independent Suppression of GABA Release Caused by a Contaminant of the Group I Metabotropic Glutamate Receptor Agonist, DHPG
    Lafourcade CA, Zhang L, Alger BE
    PLoS One. 2009 Jul 1;4(7):e6122
  2. Downregulation of Tonic GABAergic Inhibition in a Mouse Model of Fragile X Syndrome
    Curia G, Papouin T, Séguéla P, Avoli M
    Cereb Cortex. 2009 Jul;19(7):1515-20
  3. Enlarged extracellular space of aquaporin-4–deficient mice does not enhance diffusion of Alexa Fluor 488 or dextran polymers
    Xiao F, Hrabetová S
    Neuroscience. 2009 Jun 16;161(1):39-45
  4. Intracellular Zn2+ increases contribute to the progression of excitotoxic Ca2+ increases in apical dendrites of CA1 pyramidal neurons
    Vander Jagt TA, Connor JA, Weiss JH, Shuttleworth CW
    Neuroscience. 2009 Mar 3;159(1):104-14
  5. Age-Dependent Alterations of Corticostriatal Activity in the YAC128 Mouse Model of Huntington Disease
    Joshi PR, Wu NP, André VM, Cummings DM, Cepeda C, Joyce JA, Carroll JB, Leavitt BR, Hayden MR, Levine MS, Bamford NS
    J Neurosci. 2009 Feb 25;29(8):2414-27
  6. Intracellular Zn2+ Accumulation Contributes to Synaptic Failure, Mitochondrial Depolarization, and Cell Death in an Acute Slice Oxygen–Glucose Deprivation Model of Ischemia
    Medvedeva YV, Lin B, Shuttleworth CW, Weiss JH
    J Neurosci. 2009 Jan 28;29(4):1105-14
  7. Diffusion of flexible random-coil dextran polymers measured in anisotropic brain extracellular space by integrative optical imaging
    Xiao F, Nicholson C, Hrabe J, Hrabetová S
    Biophys J. 2008 Aug;95(3):1382-92
  8. Vasopressin Increases Locomotion through a V1a Receptor in Orexin/Hypocretin Neurons: Implications for Water Homeostasis
    Tsunematsu T, Fu LY, Yamanaka A, Ichiki K, Tanoue A, Sakurai T, van den Pol AN
    J Neurosci. 2008 Jan 2;28(1):228-38
  9. The effects of oleic-acid (OA) on distinct populations of neurons in the hypothalamic arcuate nucleus (ARC) are dependent on extracellular glucose levels
    Wang R, Cruciani-Guglielmacci C, Migrenne S, Magnan C, Cotero VE, Routh VH
    J Neurophysiol. 2006 Mar;95(3):1491-8
  10. Influence of Location of a Fluorescent Zinc Probe in Brain Slices on Its Response to Synaptic Activation
    Alan R. Kay and Katalin Tóth
    J Neurophysiol 95:1949-1956, 2006
  11. Bupivacaine Inhibits Activation of Neuronal Spinal Extracellular Receptor–activated Kinase through Selective Effects on Ionotropic Receptors
    Fumi Yanagidate, M.D., Gary R. Strichartz, Ph.D.
    Anesthesiology 2006; 104:805–14
  12. Cholecystokinin Activates Orexin/Hypocretin Neurons through the Cholecystokinin A Receptor
    Natsuko Tsujino, Akihiro Yamanaka, Kanako Ichiki, Yo Muraki, Thomas S. Kilduff, Ken-ichi Yagami, Satoru Takahashi, Katsutoshi Goto, and Takeshi Sakurai1
    The Journal of Neuroscience, August 10, 2005, 25(32):7459-7469
  13. Reactive oxygen species formation in the transition to hypoxia in skeletal muscle
    Zuo L, Clanton TL
    Am J Physiol Cell Physiol. 2005 Jul;289(1):C207-16
  14. Input of orexin/hypocretin neurons revealed by a genetically encoded tracer in mice
    Sakurai T, Nagata R, Yamanaka A, Kawamura H, Tsujino N, Muraki Y, Kageyama H, Kunita S, Takahashi S, Goto K, Koyama Y, Shioda S, Yanagisawa M
    Neuron. 2005 Apr 21;46(2):297-308
  15. Chronic Cocaine Administration Switches Corticotropin-Releasing Factor2 Receptor-Mediated Depression to Facilitation of Glutamatergic Transmission in the Lateral Septum,
    Jie Liu, Baojian Yu, Luis Orozco-Cabal, Dimitri E. Grigoriadis, Jean Rivier, Wylie W. Vale, Patricia Shinnick-Gallagher, and Joel P. Gallagher,
    The Journal of Neuroscience, January 19, 2005, 25(3):577-583
Integrated Components Brochure_06_7_11.pdfIntegrated Components for Live Cell Imaging Brochure
RC-27L RC-29 Wicking (2000.05.15).pdfInformation for RC-27L and RC-29 Wicking Chamber
RC-27L (080724).pdfRC-27L Chamber Instructions
perfusion_strategies.pdfPerfusion Strategies (Leinders-Zufall)