IPL-2 Core Isolated Perfused Lung System for Rat or Guinea Pig

IPL-2 Core Isolated Perfused Lung System for Rat or Guinea Pig

The IPL-2 is the most functionally diverse isolated organ system for rat and guinea pig lung, and the standard for most isolated lung preparations.

Guinea pig IPL preparations are preferred for inhalation studies while rat lungs, popular for use in pulmonary metabolism studies, are an ideal size for ease of preparation and cost-effectiveness. The basic system can be easily upgraded to add capability modules for virtually any ex vivo pulmonary assay.

Please note: For a functional unit, the core system requires the addition of a selection of a cannula set and core system options as well as a ventilator. Please see the Full Description for details.

To ensure that your system is properly configured as a functional unit that meets your application needs, please complete our Checklist and then contact Technical Services at 800-547-6766 or via email at support@hbiosci.com before placing an order. In Europe, please call +49 7665 92000 or via email at sales@hugo-sachs.de. Upgrades can also be modified to meet your specific needs.

Item# Description U.S. List Price Quantity
73-4275 IPL-2 Core System for Isolated Rat or Guinea Pig Lung, 230 V
73-4276 IPL-2 Core System for Isolated Rat or Guinea Pig Lung, 115 V
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The IPL-2 is the most functionally diverse isolated organ system for rat and guinea pig lung, and the standard for most isolated lung preparations. It can be modified to accomodate mouse lung.

Advanced Engineering
Features & Benefits
Applications
Measured Signals & Calculated Parameters
Included items
Additional Requirements for a Functional Unit
Additional Components
Options and Upgrades


Advanced Engineering

IPL-2 is a functionally diverse isolated organ perfusion system for rat and guinea pig lung. The system can be modified to accommodate mouse lung.

Guinea pig IPL preparations are preferred for inhalation studies while rat lungs, popular for use in pulmonary metabolism studies, are an ideal size for ease of preparation and cost-effectiveness. The basic system can be easily upgraded to add capability modules for virtually any ex vivo pulmonary assay. 

Ventilation 
The lung can be ventilated with a standard positive pressure ventilator (e.g. VentElite) or with the Ventilation Control Module (VCM) which allows the researcher to do positive and negative sub-atmospheric ventilation, such as in vivo studies. The type of ventilation depends on the application and aim of the study.

The preparation of the lung is done in vivo under positive pressure ventilation. The tracheal cannula, the pulmonary artery cannula and the left atrium cannula are placed in situ. Also the perfusion starts in situ. The lung remains continuously ventilated during the explantation process and is transferred into the artifical lung chamber without an interruption in ventilation. A collapsed lung never would come back to the same tidal volume compared to a non-interrupted ventilation.

When the lung is placed in the artificial thoracic chamber (and the VCM is used as ventilator), the ventilation can be changed to sub-atmospheric negative pressure to mimic the in vivo situation. Respiration rate, end-inspiratory and end-expiratory pressures can be set on the VCM. The VCM also offers sigh breath in order to keep the lung in good condition and avoid edema over longer hours.

A pneumotachometer measures the airflow in and out of the lung. From this signal the tidal volume (TV) is calcualated by software (e.g. PULMODYN).  

Perfusion 
The lung is perfused through the cannulated pulmonary artery. The perfusate is passed by means of a roller pump at constant flow or constant pressure (requires SCP module) through a heat exchanger, through a bubble trap to the pulmonary artery and finally into the lung vascular bed. The perfusate outflow is usually provided by cannulating the left atrium of the heart.

The heart remains attached to the lung but is out of function. It is just used to have an easy cannulation through the pulmonary artery and left atrium.

The IPL-2 core system contains virually everything you need to accomplish the basic perfusion and ventilation experiments, requiring only the addition of core options for ventillation and species-specific cannulae. Application-specific upgrades and options are also available.

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Features & Benefits

  • Optimized temperature conditions for the isolated lung, unique jacketed thoracic chamber
  • Operating table for non-damaging in situ preparation
  • Negative-pressure ventilation similar to in vivo condition or positive-pressure ventilation available
  • Low flow resistance and dead space volume, minimize perfusion artifacts
  • Fully thermostated lung chamber
  • Unique built-in pneumotachometer and air humidifier with small dead volume
  • Option for positive and negative pressure ventilation with one-step switch
  • Configurable periodic sigh (hyperinflation) breaths to minimize edema formation
  • Quick switch from constant flow to constant pressure for maximum application flexibility
  • Drug injection pathway built directly into pulmonary perfusate stream
  • Unique compensation system for vascular transmural pressure changes
  • More measurement parameters than any other system

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Applications

  • Investigation of ventilation and perfusion in the isolated rat or guinea pig lung 
  • Continuous measurement of respiratory mechanics (tidal volume, airway resistance, dynamic compliance) and perfusate characteristics (pressure, lung vascular resistance, pO2, pH)

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Measured Signals and Calculated Parameters

 The following signals are recorded as raw data:

  • Pulmonary artery (perfusion) pressure
  • Intrapleural (artificial thorax) pressure or tracheal pressure
  • Respiratory air flow

The following parameters are calculated from the raw data:

  • Tidal volume, minute volume
  • Peak inspiratory and expiratory airflow
  • Vascular resistance
  • Respiration rate
  • Inspiratory time and expiratory time
  • End-inspiratory and end-expiratory pressures
  • Dynamic airway resistance and compliance

* Calculations are automatic when PULMODYN data acquisition software is used.

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Included Items

IPL-2 Core System,  230 V (73-4275) includes: IPL-2 Core System, 115 V (73-4276) includes:
Item # Description Item # Description
73-2266 Basic Unit for the Rat/Guinea Pig Isolated Perfused Lung*
73-2266 Basic Unit for the Rat/Guinea Pig Isolated Perfused Lung*
73-0125 Lauda Thermostatic Circulator, Type E-103, 230 V/50 Hz, 3 L Bath Volume, Temperature Range 20 to 150°C
73-2802 Lauda Thermostatic Circulator, Type E-103, 115 V/60 Hz, 3 L Bath Volume, Temperature Range 20 to 150°C
73-3440 Jacketed Glass Reservoir for Buffer Solution, with Frit, 2.0 L 73-3440 Jacketed Glass Reservoir for Buffer Solution, with Frit, 2.0 L
73-3456 Tube Set for Jacketed Buffer Reservoir with Fluid Line Shutoff Valves 73-3456 Tube Set for Jacketed Buffer Reservoir with Fluid Line Shutoff Valves
73-0114 REGLO Analog 4-Channel MS-4/8-100, 230 VAC, 50 Hz
73-0113 REGLO Analog 4-Channel MS-4/8-100, 115 VAC, 60 Hz
73-0155 3-Stop Tygon® E-Lab Tubing, 2.79 mm ID, 12/pack, Purple/White 73-0155
 3-Stop Tygon® E-Lab Tubing, 2.79 mm ID, 12/pack, Purple/White
73-1839 3-Stop Tygon® E-Lab Tubing, 3.17mm ID, 12/pack, Black/White 73-1839 3-Stop Tygon® E-Lab Tubing, 3.17 mm ID, 12/pack, Black/White
73-0045 PLUGSYS Case, Type 603 73-0045 PLUGSYS Case, Type 603
73-2806 Servo Controller for Perfusion (SCP) 73-2806 Servo Controller for Perfusion (SCP)
73-2488  Operating Table, Size 5, for IPL-2* 73-2488  Operating Table, Size 5, for IPL-2*
Perfusion Pressure Measurements
73-0020 Low Range Blood Pressure Transducer P75 for PLUGSYS Module 73-0020 Low Range Blood Pressure Transducer P75 for PLUGSYS Module
73-1793 PLUGSYS Transducer Amplifier Module (TAM-D) 73-1793 PLUGSYS Transducer Amplifier Module (TAM-D)
Respiratory Flow Measurements
73-3882
Differential Low Pressure Transducer DLP2.5, Range  +- 2.5 cmH2O, HSE Connector
73-3882 Differential Low Pressure Transducer DLP2.5, Range  +- 2.5 cmH2O, HSE Connector
73-0065 PLUGSYS Transducer Amplifier Module (TAM-A) 73-0065 PLUGSYS Transducer Amplifier Module (TAM-A)
Artificial Thorax Pressure
73-0064
Differential Pressure Transducer MPX, Range +- 100 cmH2O, HSE Connector
73-0064 Differential Pressure Transducer MPX, Range +- 100 cmH2O, HSE Connector
73-0065 PLUGSYS Transducer Amplifier Module (TAM-A)  73-0065 PLUGSYS Transducer Amplifier Module (TAM-A) 

The basic unit includes: Plexiglass stand, jacketed lung chamber (artificial thorax), double Perspex disk that seals the chamber and contains the connectors that interface with the perfusion system, heat exchanger, humidifier and warm up system for breathing air, pressure equilibration vessel for the venous outflow, holders for the pressure transducers and all needed accessories (pulmonary artery cannula, left atrium cannula). Tracheal canula, must be purchased separately (different diameters available).

*Included Operating Table:

The operating table is specially designed for a stable and easy in situ preparation under most physiological conditions (e.g. stable perfusion pressure). It is attached to the Perspex stand and allows an exploration of the lung without interruption of ventilation and perfusion.

Surgery takes place at a hydrostatically neutral elevation, minimizing physiological hydrostatic pressure pertubations. An included plastic trough collects waste. The table is easily removed for cleaning after the lung is placed in the chamber. The operating table is 30 cm x 18.5 cm in size with four paw clamps, two thorax retractors, holder for chamber lid with heat exchanger, and ball joint for positioning.

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Additional Requirements for a Functional Unit

Negative Pressure Ventilation

For a functional unit, the core system requires the addition of one of these options (see table below) when performing both standard position- and negative-pressure ventilation studies: VCMP or VCMR-2 as well as species-specific cannulae

  • VCM modules control the ventilation of the isolated lung preparation. They allow for the physiological negative pressure ventilation of the lung and positive ventilation during preparation.
    • 30 to 100 breaths per minute
    • I:E ratio can be set between 10 and 90% in 10% steps
    • End-inspiratory, end-expiratory, sigh and negative pressures can be individually set
  • The TCM Timer Counter Module allows periodic sigh (hyperinflation) breaths to minimize edema formation
  • The Pressure-Free Gas Supply Adapter (73-3448) is included with VCMR-2 (73-4280) but can be added to VCMP (73-4279) when delivering alternative gases during negative pressure ventilation. The adapter is used to supply the trachea with a gas mixture different from room air during negative ventilation including a needle valve to adjust gas flow,

Negative Pressure Ventilation Controller 
with Pump for IPL-1 & 2
VCMP (73-4279) includes:
Utilizes 6 slot units

Negative Pressure Ventilation Controller 
with Pressure Regulator for IPL-2*

VCMR-2 (73-4280) includes:
Utilizes 6 slot units

 73-1741 PLUGSYS VCM-P Ventilation Control Module with Integral Pump  73-2795 PLUGSYS VCM-R Ventilation Control Module with Regulator 
 73-1750 PLUGSYS TCM Time Counter Module  73-1750  PLUGSYS TCM Time Counter Module
 N/A  See above  73-3448 Pressure-Free Gas Supply Adapter for Alternative Gas Supply

* Negative pressure ventilation with a pressure regulator requires a pressurized gas supply from a tank or house air in the range of 2 to 8 bar (29 to 116 PSI) and the Pressure-Free Gas Supply Adapter (73-4448). If two or more gases are to be used for ventilation we also offer a Gas Select Module GSM (73-1647) which allows switching up to four gases from tanks to the VCM-R.

Positive Pressure Ventilation (PPV-2)

Choose the PPV-2 option when only positive pressure ventilation is desired. This adapter is used with the Small Animal Ventilator, Type 683 or the VentElite . It has a side port to measure the tracheal airway pressure.

Positive Pressure Ventilation for IPL-2 (PPV-2)
includes:

 73-3635 Adapter for Positive Pressure Ventilation on IPL-2


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Additional Components

Cannulae

Small Animal Ventilator, Type 683

Comes with two piston and cylinder assemblies. Allows tidal volumes to be set from 0.5 to 5.0 cc in 0.5 cc increments and the larger cylinder allows tidal volumes of 3.0 to 30 cc in 0.3 cc increments. Respiratory rate can be set from 18 to 150 strokes per minues. Volume and rate settings can be adjusted while the ventilator is running.

VentElite

Allows tidal volumes to be set from 50 µL up to 5 ml. Touchscreen display and many advanced features.

Aerosol Nebulizer Kit (73-2111 or 73-2112*)

  • Low particle sizes (60% of the particles are below 2.5 µm; 100% of the particles are below 10 µm).
  • Requires compressed air 1.5 to 2 bars
  • No solution warming required
  • Recommended for nebulizing drugs sensitive to ultrasonics
  • Aerosol is automatically transported by compressed air at a pressure of 1.5 bar (22 PSI)

Includes:

  • Nebulizer
  • Fast connecting kit
  • ON/OFF switch form compressed air
  • All necessary tubing to connect nebulizer
  • Aerosol filter

* For two nebulizer kits

PULMODYN Data Acquisition Software

Online evaluation of a wide range of signals and classical respiration parameters.

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Options & Upgrades

Addition to Perfuse Mouse Lungs

Components to enable mouse perfused lung experiments on an IPL-2 system.

Perfusion Occlusion System

Allows double or individual arterial or venous occlusion

Real-Time Flow Measurement

Direct real-time flow measurement with inline flow probe and TTFM-2 Transit Time Flowmeter Module

Continuous Measurement of  pO2, pH and pCO2

The Universal Perfusion Solution Monitor permits precise continuous or discontinuous measurement in liquid media or perfusate of these three key parameters: pO2, pH and pCO2.

Drug Addition

These options for drug addition can be added to any isolated organ system where flow is measured or calculated and a drug must be added in a certain ratio.

Temperature Measurement

Measure perfusate temperature in any isolated perfused organ system

Deoxygenation

For deoxygenation of blood or buffers containing proteins (e.g. albumin) or erythrocytes

pH Control

Delivers CO2 to maintain pH when system is not deoxygenated with N2/CO2 gas mixture

Venous Pressure Measurement

Venous pressure transducer and amplifier for venous pressure measurement in an isolated perfused lung system

Aerosol Nebulizer

Delivers an alternative gas supply to IPL-1 with option VCMP. Puchase with alternative gas supply PRGS-1

Filtration of Recirculated Perfusate

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Specifications