IPL-1 Core Isolated Perfused Lung System for Mouse

IPL-1 Core System for Isolated Mouse Lung

The IPL-1 is an ex vivo lung perfusion system (EVLP) specifically engineered for the fragile mouse lung. The core system can easily be initially configured with various system options of upgraded later are your needs change. The core system contains all the primary equipment to accomplish basic perfusion and ventilation experiments, requiring only the addition of core options for ventilation. Application-specific upgrades and options are also available.

Due to the fragile nature of the mouse lung, its suspension from the tracheal and vascular cannulae is difficult to accomplish without the rapid occurrence of edema. In collaboration with experts in pulmonary physiology, a design was developed which allows for ventilation and perfusion with the lungs remaining in situ at a slight incline in the open thorax. This novel approach simplifies cannulation of the pulmonary artery and left atrium of the heart and dramatically reduces edema formation.

The isolated mouse lung can be ventilated under positive pressure and under subatmospheric pressure to mimic the in vivo situation as closely as possible. There are bigger differences in vascular flows seen between positive and negative ventilation. Depending on the study, a ventilated lung or a physiologic breathing lung can be simulated.

Please note: For a functional unit, the core system requires the addition of core system options for ventilation. Please see the Full Description for details.

To ensure that your system is properly configured as a functional unit that meets your application needs, please complete our Checklist and then contact Technical Services at 800-547-6766 or or via email at  support@hbiosci.com before placing an order. In Europe, please call +49 7665 92000 or email at  sales@hugo-sachs.de.

Please view our NEW comprehensive catalog!

Item# Description U.S. List Price Quantity
73-4291 IPL-1 Core System for Isolated Perfused Mouse Lung, 230 VAC
73-4292 IPL-1 Core System for Isolated Perfused Mouse Lung, 115 VAC
Login for U.S. Pricing


The IPL-1 is an ex vivo lung perfusion (EVLP) system specifically engineered for the fragile mouse lung. The core system can initially configured with various system options or upgraded later as you needs change.

The IPL-1 core system contains all the primary equipment to accomplish the basic perfusion and ventilation experiments,requiring only the addition of core options for ventilation. Application-specific upgrades and options are also available.

Advanced Engineering
Features & Benefits
Applications
Measured Signals & Calculated Parameters
Included items
Additional Requirements for a Functional Unit
Additional Components
Options and Upgrades


Advanced System Design

Due to the fragile nature of the mouse lung, its suspension from the tracheal and vascular cannulae is difficult to accomplish without the rapid occurrence of edema. In collaboration with experts in pulmonary physiology, a design was developed which allows for ventilation and perfusion with the lungs remaining in situ at a slight incline in the open thorax.. This novel approach simplifies cannulation of the pulmonary artery and left atrium of the heart and dramatically reduces edema formation.

Most of the studies with isolated perfused lungs have been performed with lungs obtained from rats, guinea pigs, rabbits or dogs. The interaction of the immune system with the lung is currently an area of great interest. The mouse is the best characterized rodent species with respect to its immune system. Immunologic probes such as antibodies and cytokines are more widely available for mice versus other species. LIkewise, the mouse if the most commonly used animal model in the biomedical research community due to the wealth of genetically modified variants for the species.

Ventilation 

The HSE IPL systems for EVLP are the only commercially available systems that allow the investigator to select either positive or negative pressure ventilation. Positive pressure ventilation can be used in studies that seek to mimic clinical EVLP systems. Sub-atmospheric pressure ventilation, due to the substantial difference in vascular flow rates, allows one to more precisely mimic the in vivo physiological conditions. The lung is placed inthe chamber and ventilated by either positive airway pressure or negative artificial thoracic pressure. It is connected through the tracheal cannula tothe pneumotachometer for measurement of respiratory air flow. Using either positive or negative pressure, the respiration rate, the inspiratory pressure and the end-expiratory pressure can be adjusted with separate controls using the Ventilation Control Module (VCM). Positive only ventilation using a regular MiniVent ventilator is also possible. The surgery is always performed under positive ventilation to avoid the lung collapsing. Switching over to subatmospheric ventilation only requires you to attach the chamber lid and switch over a stopcock. Switching back to positive ventilation is always possible.

Perfusion 

The pulmonary artery is cannulated to provide perfusion. The perfusate is passed by means of a roller pump at constant flow or constant pressure (using the SCP module) through the heat exchanger, through a bubble trap to the pulmonary artery and finally into the lung vascular bed. The perfusate outflow is usually provided by cannulating the left atrium of the heart. The non-functional lung remains in the thoracic cavity and allows easier cannula placement for the effluent.

Back to Top


Features & Benefits

  • Exclusive artificial thorax chamber for isolated lung with integrated changeover system—quick switch between simple positive-pressure ventilation and physiological subatmospheric (negative) pressure ventilation
  • Integrated surgery table—reduces damage during preparation
  • Pressure-balancing vessel—eliminates transmural pressure difference during sub-atmospheric ventilation; allows for simulation of hypertensive cardiac afterload
  • Built-in, warmed pneumotachometer—minimal dead space volume
  • Low flow resistance and dead space volume—minimize perfusion artifacts
  • Milled infusion and pressure measurement paths—allow access to closed chamber
  • Built-in humidifier frit—prevents lung drying
  • Moist lung chamber has access ports for additional measurements or other sensors
  • Simple add-on aerosol nebulizer
  • Optional multi-gas inlet adapter for hyperoxic, hypercapnic, anesthetic or other alternative gas studies
  • Drug injection pathway built directly into pulmonary perfusate stream

Back to Top


Applications

  • Investigation of ventilation and perfusion in the isolated mouse lung 
  • Continuous measurement of respiratory mechanics (respiration rate,inspiratory and expiratory air flow, tidal volume, minute volume, dynamic airway resistance, dynamic lung compliance) and perfusion characteristics (pulmonary artery pressure, left atrium pressure, lung vascular resistance,pO2, pCO2, pH)
  • Drug testing on respiration and vascular parameters

Back to Top


Measured Signals and Calculated Parameters

  • Pulmonary artery (perfusion) pressure
  • Perfusion flow
  • Intrapleural (artificial thorax) pressure or tracheal pressure
  • Respiratory flow

The following parameters are calculated from the raw data:

  • Respiration rate
  • Peak inspiratory and expiratory airflow
  • Tidal volume, minute volume
  • Vascular resistance
  • End-inspiratory and end-expiratory pressures
  • Dynamic airway resistance and dynamic lung compliance
  • Inspiratory time and expiratory time (Software Option)

* Calculations are automatic when PULMODYN data acquisition software is used.

Back to Top


Included Items

IPL-1 Core System, Basic, 230 V (73-4291) includes: IPL-1 Core System, Basic, 115 V (73-4292) includes:
Item # Description Item # Description
73-2329 Basic Unit for the Mouse Isolated Perfused Lung*
73-2329 Basic Unit for the Mouse Isolated Perfused Lung*

73-4544 TC120 Thermocirculator, complete with 5 L stainless steel bath and lid, 220 V
73-4545 TC120 Thermocirculator,complete with 5 L stainless steel bath and lid, 120 V
73-3436 Water Jacketed Glass Perfusate Reservoir with Oxygenating Frit, 0.5 L 73-3436 Water Jacketed Glass Perfusate Reservoir with Oxygenating Frit, 0.5 L

73-3456 Tube Set for Jacketed Buffer Reservoir with Fluid Line Shutoff Valves 73-3456 Tube Set for Jacketed Buffer Reservoir with Fluid Line Shutoff Valves
73-0114 REGLO Analog 4-Channel Peristaltic, 230 VAC, 50 Hz
73-0113 REGLO Analog 4-Channel Peristaltic Pump, 115 VAC, 60 Hz
73-1825 3-Stop Tygon® E-Lab Tubing, 0.89 mm ID, 12/pack, Orange/Orange 73-1825
 3-Stop Tygon® E-Lab Tubing, 0.89 mm ID, 12/pack, Orange/Orange
73-0126 3-Stop Tygon® E-Lab Tubing, 1.22 mm ID, 12/pack, Red/Grey 73-0126 3-Stop Tygon® E-Lab Tubing, 1.22 mm ID, 12/pack, Red/Grey
73-0500 Lab Stand with Triangular Base Plate, 30 cm Rod Length (one block clamp included) 73-0500  Lab Stand with Triangular Base Plate, 30 cm Rod Length (one block clamp included)
73-0566 Plexiglass Block Clamp to mount 73-0566 Bar onto rod (up to 9 mm OD)
73-0566 Plexiglass Block Clamp to mount 53-0566 Bar onto rod (up to 9 mm OD)

73-0045 PLUGSYS Case, Type 603 73-0045 PLUGSYS Case, Type 603
73-2806 Servo Controller for Perfusion (SCP) 73-2806 Servo Controller for Perfusion (SCP)
Perfusion Pressure Measurements
73-0020 Low Range Blood Pressure Transducer P75 for PLUGSYS Module 73-0020 Low Range Blood Pressure Transducer P75 for PLUGSYS Module
73-1793 PLUGSYS Transducer Amplifier Module (TAM-D) 73-1793 PLUGSYS Transducer Amplifier Module (TAM-D)
Respiratory Airflow Measurements
73-3882
Differential Low Pressure Transducer DLP2.5, Range  +- 2.5 cmH2O, HSE Connector
73-3882 Differential Low Pressure Transducer DLP2.5, Range  +- 2.5 cmH2O, HSE Connector
73-0065 PLUGSYS Transducer Amplifier Module (TAM-A) 73-0065 PLUGSYS Transducer Amplifier Module (TAM-A)
Artificial Thorax or Tracheal Pressure Measurements
73-0064
Differential Pressure Transducer MPX, Range +- 100 cmH2O, HSE Connector
73-0064 Differential Pressure Transducer MPX, Range +- 100 cmH2O, HSE Connector
73-0065 PLUGSYS Transducer Amplifier Module (TAM-A)  73-0065 PLUGSYS Transducer Amplifier Module (TAM-A) 

The Base Unit (73-2329) includes:

 Temperature-controlled negative pressure lung chamber (artificial thorax) with cover including Venturi jet, positive pressure ventilation head, air humidifier, pneumotachometer, connectors to interface with the perfusion system, pressure equilibration vessel for venous flow and all necessary accessories (tracheal cannulae, pulmonary artery cannulae, left atriumcannulae). Optional Y adapter available for positive pressure ventilation.

Mouse Cannulae (included with Base Unit):

  • Tracheal Cannula for Mouse, ID 1.0 mm, OD 1.3 mm, L 20 mm (73-4181)
  • Pulmonary Artery Cannula for Mouse, Stainless Steel, ID 1.0 mm, OD1.3/1.6 mm, L 28 mm (73-0723)
  • Atrial Cannula for Mouse, ID 1.0 mm, OD 1.6 mm, L 24 mm (73-0724)

Back to Top


Additional Requirements for a Functional Unit

For a functional IPL-2 unit the core system requires a suitable ventilation system.For only positive ventilation a mouse ventilator can be used. If positive and sub-atmospheric pressure ventilation is required, the Ventilation Control Module must be purchased.

Back to Top


Additional Components

PULMODYN Data Acquisition Software

Online evaluation of a wide range of signals and classical respiration parameters.

Back to Top


Options & Upgrades

Venous Pressure Measurement 

Venous pressure transducer and amplifier for venous pressure measurement in an isolated perfused lung system.

Perfusate Deoxygenation

For deoxygenation of blood or buffers containing proteins (e.g. albumin) or erythrocytes.

pH Control

Delivers CO2 to maintain pH when system is not deoxygenated with N2/CO2 gas mixture.

Perfusion Solution Monitoring

The Universal Perfusion Solution Monitor permits precise continuous or discontinuous measurement in liquid media or perfusate of these three key parameters: pO2, pH and pCO2.

Temperature Measurement

Measure perfusate temperature in any isolated perfused organ system.

Perfusat Filtration

For filtration of recirculated perfusate.

 

Aerosol Application

Add-on to deliver aeroxsol to the isolated lung.

Drug Addition

For accurate drug addition using a syringe pump. Additional option for flowcontrolled drug addition, where flow is measured (or calculated) and a drugmust be added in a certain ratio. 

ack to Top

Specifications
DOCUMENT DESCRIPTION
2018-HSE-HA-ISO-Lung.pdf2018 Isolated Lung Perfusion Systems Catalog/td>