IH5-BI Biventricular Working Heart System for Rat, Guinea Pig and Rabbit

IH5-BI Biventricular Working Heart System for Rat, Guinea Pig and Rabbit

IH-BI Biventricular Working Heart System for rat, guinea pig and rabbit provides a valuable tool for the researcher who wants to study whole-heart cardiac function.

The Biventricular Isolated Working Heart perfusion system utilizes patented flow resistance and compliance chambers to faithfully mimic the in vivo cardiac preloads and afterloads for both normal and diseased states in medium to large models (rat, guinea pig, rabbit). The exclusive features of the system create the most physiological isolated heart environment closely resembling the actual resistance of the peripheral vasculature while allowing the entire heart to work as it does in vivo, setting a new standard for isolated heart research. 

To ensure that your system is properly configured as a functional unit that meets your application needs, please complete our Checklist and then contact Technical Services at 800-547-6766 or via email at support@hbiosci.com before placing an order. In Europe, please call +49 7665 92000 or via email at sales@hugo-sachs.de. Upgrades can also be modified to meet your specific needs.

Item# Description U.S. List Price Quantity
73-4420 IH5-BI Core Biventricular Working Heart System for Rat, Guinea Pig and Rabbit Heart, 230 V
73-4419 IH5-BI Core Biventricular Working Heart System for Rat, Guinea Pig and Rabbit Heart, 115 V
73-4450 Rat/Guinea Pig Biventricular Cannula Set
73-4451 Rabbit Biventricular Cannula Set
Login for U.S. Pricing


The IH-5 Langendorff system can be upgraded to the Biventricular Working Heart System (IH5-BI). For use with rat, guinea pig and rabbit hearts, it is a valuable tool for the researcher who wants tostudy whole heart cardiac function.

The complete system contains all the primary equipment you need to accomplish the basic biventricular working heart experiment, only requiring the addition of species-specific cannulae. Many options are available for the IH5-BI system to extend its capabilities. 

Advanced System Design
Features & Benefits
Applications
Measured Signals & Calculated Parameters
Included items
Species-Specific Options
Specialized Applications & Upgrades


Advanced System Design

The IH5-BI System utilizes patented flow resistance and compliance chambers to faithfully mimic the in vivo cardiac preloads and afterloads for both normal and diseased states in medium to large rodent models. The exclusive features of the system create a physiological isolated heart environment that closely resembles the actual resistance of the peripheral vasculature while allowing the entire heart to work as it does in vivo.This IH5-BI allows for in-depth ex vivo studies of the effects of pulmonary artery hypertension (PAH), hypertrophy (RVH), COPD, ARCV, emphysema and a variety of other diseases characterized by pulmonary vascular dysfunctionand right heart pathophysiology. It allows measurement of a wide range of cardiovascular parameters with unsurpassed fidelity, such as single ECG Lead II and single epicardial monophasic action potential (MAP). Integration of high fidelity pressure-volume catheters for right and left ventricle pressure-volume loop measurement is also available. 

Learn about the differences between Working (Ejecting) Heart and Biventricular Working Heart.

Back to Top


Features & Benefits

  • Combines the advantages of an isolated organ preparation with in situ-like perfusion features
  • Allows assessment of external heart work under adjustable load for both right and left heart
  • Allows a more comprehensive monitoring of functional parameters, the calculation of the external heart work and mechanical efficiency, and the highest sensitivity for various experimental manipulation in ex vivo isolated heart preparations
  • Allows rapid and easy switching between biventricular working heart, left working heart and Langendorff modes
  • All of the same features and benefits of the IH-5—compact, no high water columns, low resistance laminar flow paths and low system volume

Back to Top


Applications

  • All applications for the standard IH-5 Working Heart System
  • Calcium homeostasis
  • Drug effects on right heart electrophysiology and contractility 
  • Ischemia/reperfusion effects on right heart
  • Effects of right ventricular hypertrophy on cardiac function

Back to Top


Measured Signals and Calculated Parameters

The following signals can be recorded:

  • Left atrial preload pressure (left ventricular filling pressure)
  • Right atrial preload pressure (right ventricular filling pressure)
  • Afterload pressure (aortic pressure for calculation of systolic and diastolic pressure)
  • Right ventricular afterload (pulmonary artery pressure)
  • Left ventricular pressure or pressure volume loops
  • Right ventricular pressure or pressure volume loops
  • Cardiac output (left atrial flow), aortic, right atrial flow
  • Coronary flow (subtraction of aortic flow from CO or by subtraction of right atrial flow from pulmonary artery flow)

Back to Top


Included Items

IH5-BI Core System, Basic, 230 V (73-4420) includes: IH5-BI Core System, Basic, 115 V (73-4419) includes:
Item # Product Name Item # Product Name
73-4238 Biventricular Working Heart Base Unit 73-4238 Biventricular Working Heart Base Unit
73-3611 Heart Chamber, JacketedGlass Vessel, ID = 145 mm,with Bottom Drain 73-3611 Heart Chamber, JacketedGlass Vessel, ID = 145 mm,with Bottom Drain
73-4544 TC120 Thermocirculator, with 5 L stainless steel bath and lid, 220 V 73-4545 TC120 Thermocirculator, with 5 L stainless steel bath and lid, 120 V
73-0116 Peristaltic Pump MCP-SB2, 230 VAC
73-0115 Peristaltic Pump MCP-SB2, 115 VAC
73-6432 Ecoline Peristaltic Pump, VC-MS/CA8-6, 8 Channels, 230 VAC, 50 Hz 73-6433 Ecoline Peristaltic Pump, VC-MS/CA8-6, 8 Channels,115 VAC, 60 Hz
73-0155 3-Stop Tygon® E-Lab Tubing, 2.79 mm ID, 12/pack, Purple/White 73-0155 3-Stop Tygon® E-Lab Tubing, 2.79 mm ID, 12/pack, Purple/White
73-0322 Jacketed Glass Reservoir for Buffer Solution, with Frit, 6 L 73-0322 Jacketed Glass Reservoir for Buffer Solution, with Frit, 6 L
73-0045 PLUGSYS Case, Type 603 73-0045 PLUGSYS Case, Type 603
73-1793 PLUGSYS Transducer Amplifier Module (TAM-D) 73-1793 PLUGSYS Transducer Amplifier Module (TAM-D)
73-3862 Blood Pressure Transducer (APT300) 73-3862 Blood Pressure Transducer (APT300)
73-0020 Blood Pressure Transducer (P75) for PLUGSYS Module 73-0020 Blood Pressure Transducer (P75) for PLUGSYS Module
73-0065 PLUGSYS Transducer Amplifier Module (TAM-A), 2 included 73-0065 PLUGSYS Transducer Amplifier Module (TAM-A), 2 included

 

The IH5-BI Base Unit (73-4238) includes:

Plexiglass stand, aorta link unit with Windkessel, adjustable artificial flow resistance for adjusting the aortic afterload pressure (with pressure gauge), preheating coil for Langendorff mode, holder for pressure transducers (aortic/perfusion pressure), tubing for in situ preparation with adapter. Also included are a left atrium cannulating system with preload reservoir and preheating coil, movable atrium connection adapter and holder for P75 (preload pressure),a column for right atrium cannulation, movable right atrium connection adapter, holder for P75 (right atrium pressure), and a right ventricle afterload system (simulates lung resistance). All cannulae must be ordered separately and depends on your animal model.

Back to Top


Required Species-Specific Additions

For a functional unit the IH5-BI Core Systems require one of these species-specific cannula sets:

Rat/Guinea Pig Biventricular Cannula Set (73-4450) includes:

  • Aortic Cannula Set for rat/guinea pig hearts, 2.5 mm OD and 3.0 mm OD
  • Left Atrium Cannula, 2.3 mm OD
  • Right Atrium Cannula, 2.3 mm OD
  • Pulmonary Artery Cannula Set, 2.5 mm OD plus Mini-Ball Joint Mounting Kit

Rabbit Biventricular Cannula Set (73-4451) includes:

  • Aortic Cannula for rabbit hearts, 3.0, 4.0 and 5.0 mm OD
  • Left Atrium Cannula, 2.3 mm OD
  • Right Atrium Cannula, 2.3 mm OD
  • Pulmonary Artery Cannula Set, 2.5 mm OD plus Mini-Ball Joint Mounting Kit

Back to Top


Specialized Applications & Upgrades

All options compatible with the IH-5 Working Heart System are compatible with the IH5-BI Biventricular Working HeartSystem, excluding Multi-ECG and Multi-MAP. 

Back to Top

Specifications