IH5-BI Biventricular Working Heart System for Rat, Guinea Pig and Rabbit

IH5-BI Biventricular Working Heart System for Rat, Guinea Pig and Rabbit

IH-BI Biventricular Working Heart System for rat, guinea pig and rabbit provides a valuable tool for the researcher who wants to study whole-heart cardiac function.

The Biventricular Isolated Working Heart perfusion system utilizes patented flow resistance and compliance chambers to faithfully mimic the in vivo cardiac preloads and afterloads for both normal and diseased states in medium to large models (rat, guinea pig, rabbit). The exclusive features of the system create the most physiological isolated heart environment closely resembling the actual resistance of the eripheral vasculature while allowing the entire heart to work as it does in vivo, setting a new standard for isolated heart research. 

To ensure that your system is properly configured as a functional unit that meets your application needs, please complete our Checklist and then contact Technical Services at 800-547-6766 or via email at support@hbiosci.com before placing an order. In Europe, please call +49 7665 92000 or via email at sales@hugo-sachs.de. Upgrades can also be modified to meet your specific needs.

Item# Description U.S. List Price Quantity
73-4420 IH5-BI Basic Biventricular Working Heart System for Rat, Guinea Pig and Rabbit Heart, 230 V
73-4419 IH5-BI Basic Biventricular Working Heart System for Rat, Guinea Pig and Rabbit Heart, 115 V
73-4450 Rat/Guinea Pig Biventricular Cannula Set (BIV-CAN-R/GP)
73-4451 Rabbit Biventricular Cannula Set (BIV-CAN-RAB)
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IH-BI Biventricular Working Heart System for rat, guinea pig and rabbit provides a valuable tool for the researcher who wants to study whole-heart cardiac function.

Advanced Engineering
Features & Benefits
Applications
Measured Signals & Calculated Parameters
Included items
Species-Specific Options
Additional Components
Options and Upgrades


Advanced Engineering

The Biventricular Isolated Working Heart perfusion system utilizes patented flow resistance and compliance chambers to faithfully mimic the in vivo cardiac preloads and afterloads for both normal and diseased states in medium to large models (rat, guinea pig, rabbit). The exclusive features of the system create the most physiological isolated heart environment closely resembling the actual resistance of the peripheral vasculature while allowing the entire heart to work as it does in vivo, setting a new standard for isolated heart research. This fully-integrated system allows for in-depth ex vivo studies of the effects of Pulmonary Artery Hypertension (PAH), Hypertrophy (RVH), COPD,ARCV, Emphysema and a variety of other diseases characterized by pulmonary vascular dysfunction and right heart pathophysiology.

The system allows measurement of a wide range of cardiovascular parameters with unsurpassed fidelity, such as single ECG Lead II and Single Epicardial Monophasic Action Potential. Integration of High Fidelity Pressure Volume Catheters for right and left ventricle PV Loop Measurement is also available.

The complete system contains virtually everything you need to accomplish the basic Biventricular Working Heart experiment, only requiring the addition of the appropriate species-specific option. 

Learn about the differences between Working (Ejecting) Heart and Biventricular Working Heart.

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Features & Benefits

  • Combines the advantages of an isolated organ preparation with in situ-like perfusion features
  • Allows a more comprehensive monitoring of functional parameters, the calculation of the external heart work and mechanical efficiency, and the highest sensitivity for various experimental manipulation in ex vivo isolated heart preparations
  • Allows rapid and easy switching between Biventricular Working Heart, left Working Heart and Langendorff modes
  • All of the same features and benefits of the IH-5—compact, no high water columns, low resistance laminar flow paths and low system volume
  • Assessment of external heart work under adjustable load for both right and left heart
  • Modular design to integrate more applications in the future
  • Solid state perfusion technology mimics in vivo perfusion and prevents measurement artifact

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Applications

  • Calcium homeostasis
  • Drug effects on right heart electrophysiology and contractility 
  • Ischemia/reperfusion effects on right heart
  • Effects of right ventricular hypertrophy on cardiac function
  • Whole heart imaging

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Measured Signals and Calculated Parameters

The following signals can be recorded:

  • Left atrial preload pressure (left ventricular filling pressure)
  • Right atrial preload pressure (right ventricular filling pressure)
  • Afterload pressure (aortic pressure for calculation of systolic and diastolic pressure)
  • Right ventricular afterload (pulmonary artery pressure)
  • Left ventricular pressure or pressure volume loops
  • Right ventricular pressure or pressure volume loops
  • Cardiac output (Left atrial flow), aortic, right atrial flow
  • Coronary flow (subtraction of aortic flow from CO or by subtraction of right atrial flow from pulmonary artery flow)

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Included Items

IH5-BI Core System, Basic, 230 V (73-4420) includes: IH5-BI Core System, Basic, 115 V (73-4419) includes:
73-4238 Biventricular Working Heart Base Unit 73-4238 Biventricular Working Heart Base Unit
73-4544 Thermostatic Circulator TC120-ST5, Bath Volume 5 L, 230 V/50 Hz 73-4545 Thermostatic Circulator TC120-ST5, Bath Volume 5 L, 115 V/60 Hz
73-2448 REGLO Analog 2 Channel MS-2/8-160, 230 VAC, 50 Hz
73-2952 REGLO Analog 2 Channel MS-2/8-160, 115 VAC, 50 Hz
73-0045 PLUGSYS Case, Type 603 73-0045 PLUGSYS Case, Type 603
73-1793 PLUGSYS Transducer Amplifier Module (TAM-D) 73-1793 PLUGSYS Transducer Amplifier Module (TAM-D)

73-0065 PLUGSYS Transducer Amplifier Module (TAM-A) (Does not include balloon) 73-0065 PLUGSYS Transducer Amplifier Module (TAM-A) (Does not include balloon)

73-3862 APT300 Pressure Transducers (2 included) 73-3862 APT300 Pressure Transducers (2 included)
73-3869 Holder for APT300 Transducer, 8 mm Rod, Length 75 mm 73-3869 Holder for APT300 Transducer, 8 mm Rod, Length 75 mm
73-0566 Perspex Block Clamp to mount transducer onto rod (up to 9 mm OD) 73-0566 Perspex Block Clamp to mount transducer onto rod (up to 9 mm OD)

* The IH-SR Base Unit (73-4991) includes Plexiglas stand, aortic block with aorta link unit with Windkessel, heat exchanger, jacketed heart chamber, needle valve for adjusting gas flow, holder for transducer for perfusion pressure, tubings. Aortic cannula and buffer reservoir must be ordered separately.

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Required Species-Specific Additions

For a functional unit the core systems require one of these species-specific cannula sets:

BIV-CAN-R/GP Rat/Guinea Pig Biventricular Cannula Set (73-4450) includes:

  • Aortic Cannula Set for rat/guinea pig hearts, 2.5 mm OD and 3.0 mm OD
  • Left Atrium Cannula, 2.3 mm OD
  • Right Atrium Cannula, 2.3 mm OD
  • Pulmonary Artery Cannula Set, 2.5 mm OD plus Mini-Ball Joint Mounting Kit

BIV-CAN-RAB Rabbit Biventricular Cannula Set (73-4451) includes:

  • Aortic Cannula for rabbit hearts, 3.0, 4.0 and 5.0 mm OD
  • Left Atrium Cannula, 2.3 mm OD
  • Right Atrium Cannula, 2.3 mm OD
  • Pulmonary Artery Cannula Set, 2.5 mm OD plus Mini-Ball Joint Mounting Kit

Additional Components

Please contact our technical team for help.

ISOHEART Data Acquisition Software

Stimulator and Electrodes for Pacing 

Aortic Cannulae

Buffer Reservoirs

Left Ventricular Pressure Kits (Balloons)

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Options & Upgrades

All options compatible with the IH-5 Working Heart System are compatible with the IH5-BI Biventricular Working HeartSystem, excluding Multi-ECG and Multi-MAP. 

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Specifications