IH-SR Core System for Isolated Small Rodent Heart

IH-SR Isolated Perfused Heart System for Small Rodents

The IH-SR Langendorff Core System is the starting point for all isolated perfused heart experiments on small rodents such as mouse, rat and guinea pig in the Langendorff retrograde perfusion mode.

The core Langendorff system can be easily upgraded to a working heart-ready (ejecting heart) configuration any time later.

Because of its larger heart chamber, this system is well-suited for applications that require ECG and MAP recordings in addition to LVP and pacing. The fully closing heart chamber allows for precise temperature control, making it the optimal choice for mouse heart perfusion. 

Please Note: A fully functional IH-SR system requires the addition of core system options and other components. Other options and upgrades enable specific measurements or applications. Please see the Full Description for details.

To ensure that your system is properly configured as a functional unit that meets your application needs, please complete our Checklist and then contact Technical Services before placing an order. In the US and outside of Europe, call 800-547-6766 or email support@hbiosci.com. In Europe, please call (49) 7665-92000 or email sales@hugo-sachs.de.

Item# Description U.S. List Price Quantity
73-4343 IH-SR Core System for Isolated Small Rodent Heart, 230 VAC
73-4344 IH-SR Core System for Isolated Small Rodent Heart, 115 VAC
Login for U.S. Pricing


The IH-SR Langendorff Core System is the starting point for all isolated perfused heart experiments on small rodents such as mouse, rat and guinea pig in the Langendorff retrograde perfusion mode.

Advanced Engineering
Features & Benefits
Applications
Measured Signals & Calculated Parameters
Included items
Additional Requirements for a Functional Langendorff Unit
Additional Components
Options and Upgrades


Advanced Engineering

Advanced engineering provides ease-of-use and exceptional stability of measurements while maintaining the flexibility to upgrade to more advanced capabilities. The IH-SR is a modular system which can be easily expanded to meet your changing application needs. 

Like our other Isolated Perfused Heart Systems, we do not use high water-columns to achieve a constant pressure and rely on instead other safer methods (Starling Resistor or Feedback-based Perfusion Controller) that keep every portion of your system within easy reach. Because the Aortic Block is fully enclosed in the water-jacketed upper and lower IH-SR chamber and precision milled from thermally stable Perspex, the temperature control of the IH-SR has the highest level of stability of all of the IH systems.

Because of its larger heart chamber, this system is well-suited for applications that require ECG and MAP recordings in addition to LVP and pacing. The fully closing heart chamber allows for precise temperature control, making it the optimal choice for mouse heart perfusion. 

The IH-SR base system contains virtually everything you need to accomplish the basic Langendorff experiment, only requiring the addition of the appropriate species-specific option, the method of constant pressure perfusion, and the desired data acquisition method. It is capable of being expanded to a full ejecting Working Heart (Neely) system, accommodating aortic flows up to 60 ml/min. Application-specific upgrades and options are also available.

Back to Top


Features & Benefits

  • Langendorff (Retrograde) and optional Working (Ejecting) Heart perfusion in a single system
  • Compact design—optimized individually for mouse, rat or guinea pig
  • Constant pressure or constant flow perfusion in one unit—easily switch between the two modes without changes in tubing setup
  • Does not require wall-installed high water columns—suitable for hearts from hypertensive rats (perfusion pressures up to 300 mmHg are possible)
  • Unique integrated small volume aortic block with built-in bubble trap (immediately above the aortic cannula) and windkessel
  • Large fully-heated, closed heart chamber—important to maintain heart temperature
    • Natural physiological environment for the isolated heart—optimized temperature conditions
    • Hearts are kept alive for hours in a very stable physiologic environment
    • Easy-to-use with smooth open and closed operation
  • All electrodes, catheters and probes are fully enclosed  in the unique closed jacketed chamber—easy, direct access to the heart and to maintain physiological conditions while performing the experiments.
  • Low dead space volume—fluid injection pathway directly on top of the aortic cannula into aortic stream
  • Unique cannula design
    • Cannula resistance is optimized according to Hagen-Poiseuilles physical law
    • Different cannula sizes available, all metal, no fragile glass cannulae
  • Proprietary mini holders—allow easy and stress-free positioning of electrodes, catheters and probes

Back to Top


Applications

  • Study of myogene autoregulation with the addition of Direct Coronary Flow Measurement Option    
  • Testing with inotropic substances
  • Testing of lusitrope substances
  • Testing of vasoactive substances
  • Cardiac rhythm tests
  • Ischemia/hypoxia studies
  • Refractory period studies
  • Ischemia/reperfusion injury studies
  • Cardioplegia studies
  • Cardiac preconditioning
  • Cardiovascular screening performance
  • Electrophysiology studies
  • Phenotyping of transgenic animals 
  • Drug compound screening
  • Toxicology studies
  • Biochemical tests

Back to Top


Measured Signals and Calculated Parameters

 The following signals are recorded as raw data in retrograde Langendorff perfusion:

  • Isovolumetric left ventricular pressure (balloon method)
  • Aortic (perfusion) pressure
  • Coronary flow*

The following parameters are calculated from the raw data (using the ISOHEART data acquisition software):

  • dLVP/dt, dLVP/dt Max, dLVP/dt Min, Contractility Index
  • Systolic and Diastolic LVP
  • Heart Rate
  • Mean Perfusion Pressure
  • Mean Perfusion Flow*
  • Coronary Resistance*

* This parameter is based on indirect flow measurement with option IHSR-SCP (SCP controller) or direct ultrasound flow measurement with the TTFM-2 flowmeter and suitable flow probe.

Back to Top


Included Items

IH-SR Core System, Basic, 230 V (73-4343) includes: IH-SR Core System, Basic, 115 V (73-4344) includes:
73-4991 IH-SR Base Unit for Small Rodent Hearts (Up to 800 g)* 73-4991 IH-SR Base Unit for Small Rodent Hearts (Up to 800 g)*
73-4544 Thermostatic Circulator TC120-ST5, Bath Volume 5 L, 230 V/50 Hz 73-4545 Thermostatic Circulator TC120-ST5, Bath Volume 5 L, 115 V/60 Hz
73-2448 REGLO Analog 2 Channel MS-2/8-160, 230 VAC, 50 Hz
73-2952 REGLO Analog 2 Channel MS-2/8-160, 115 VAC, 50 Hz
73-0045 PLUGSYS Case, Type 603 73-0045 PLUGSYS Case, Type 603
73-1793 PLUGSYS Transducer Amplifier Module (TAM-D) 73-1793 PLUGSYS Transducer Amplifier Module (TAM-D)

73-0065 PLUGSYS Transducer Amplifier Module (TAM-A) (Does not include balloon) 73-0065 PLUGSYS Transducer Amplifier Module (TAM-A) (Does not include balloon)

73-3862 APT300 Pressure Transducers (2 included) 73-3862 APT300 Pressure Transducers (2 included)
73-3869 Holder for APT300 Transducer, 8 mm Rod, Length 75 mm 73-3869 Holder for APT300 Transducer, 8 mm Rod, Length 75 mm
73-0566 Perspex Block Clamp to mount transducer onto rod (up to 9 mm OD) 73-0566 Perspex Block Clamp to mount transducer onto rod (up to 9 mm OD)

* The IH-SR Base Unit (73-4991) includes Plexiglas stand, aortic block with aorta link unit with Windkessel, heat exchanger, jacketed heart chamber, needle valve for adjusting gas flow, holder for transducer for perfusion pressure, tubings. Aortic cannula and buffer reservoir must be ordered separately.

For a functional unit, species-specific core options and pressure controller systems must be added.

Back to Top


Additional Requirements for a Functional Langendorff Unit

An IH-SR Langendorff basic functional unit requires the addition of core options to the selected core system (either 73-4343 or 73-4344). Specifically, the core system requires the addition of a selection of one of the perfusion pressure controllers (SCP or STARLING-IHSR) and one of the species-specific options indicated in the table below:

 Langendorff Only Configuration
 (Add these core options to 73-4343 or 73-4344)
 Langendorff Working Heart-Ready Configuration
(Add these core options to 73-4343 or 73-4344)
 Species Specific Addition (choose one or both)
• 73-4019 IHSR-MOUSE
• 73-4020 IHSR-RGP

 Species Specific Addition (choose one or both)
• 73-4019 IHSR-MOUSE
• 73-4020 IHSR-RGP

 Constant Pressure/Flow Controller (required)
• 73-2806 SCP PLUGSYS Servo Controller Module 

 Perfusion Pressure Control (required)
• 73-4346 STARLING-IHSR Starling Pressure Controller

Back to Top


Additional Components

Please contact our technical team for help.

ISOHEART Data Acquisition Software

Stimulator and Electrodes for Pacing 

Aortic Cannulae

Buffer Reservoirs

Left Ventricular Pressure Kits (Balloons)

Back to Top


Options & Upgrades

IH-SR Core System Options

 Options for required additions to the IH-SR Core System for species-specific Langendorff Only or Langendorff Working Heart-Ready configurations.

Working Heart Upgrade

The IH-SR Langendorff Core System is prepared for upgrade to a working ejecting heart  according to Neely later on. To upgrade an IH-SR Lagendorff Core System to a IH-SR Working Heart System, add the Working Heart Upgrade and one of the species-specific additions. Researchers who know they will be upgrading to working ejecting heart in the future may start with a Langendorff Working Heart-Ready configuration. If you intend to measure coronary flow, you need a TTFM-2 flowmeter and a species suitable flowmeter. (SCP cannot be used for Working Heart.)

Langendorff Only configuration can easily be modified to a Langendorff Working Heart-Ready configuration. Please contact our technical team for help!

Flow Measurement Options for IH-SR Working Heart

Flow measurement can be accomplished in an IH-SR unit using two methods: 

  • Indirect measurement by controlling the pump sped of a roller pump (SCP controller)
  • Direct measurement with Ultrasound Transit Time Technic (TTFM-2 module)

Cell Isolation Upgrade for IH-SR

Add this upgrade to a working IH-SR system for cell isolation/cell extraction applications.

Oxygenating System Option for IH-SR (for foaming solutions)

Choose this option if you are using buffer supplemented with albumin, fatty acids, washed erythrocytes, or other foaming additives.

Cannulating Options for IH-SR (for metabolic studies)

Cannulae, preparation dish and effluate funnel for effluent collection from an IH-SR system for later metabolic studies on your analysis equipment.

LVP Measurement Option for IH-SR Working Heart

Easily allows introduction of a tip pressure catheter directly into the left ventricle via the adapter port and the aorta, rather than via apical puncture.

Pressure-Volume Measurement Option for IH-SR Working Heart

Easily allows introduction of a pressure-volume loop catheter directly into the left ventricle via an adapter port and the aorta.

Continuous Measurement of  pO2, pH and pCO2

The Universal Perfusion Solution Monitor permits precise continuous or discontinuous measurement in liquid media or perfusate of these three key parameters: pO2, pH and pCO2.

Increased Preload Pressure Option for IH-SR Working  Heart

Choose this option when you need to create left atrial preload pressures higher than 11 mmHg. (Gottlieb Valve)

Single-Lead ECG and MAP Measurement

For single-lead ECG and MAP on functional IH-5 systems, one ECG lead is combined with one MAP electrode.

Drug Addition

These options for drug addition can be added to any isolated organ system where flow is measured or calculated and a drug must be added in a certain ratio.

Temperature Measurement

Measure perfusate temperature in any isolated perfused organ system

Filtration of Recirculated Perfusate

Back to Top

Specifications