IH-SR Core System for Isolated Small Rodent Heart

IH-SR Isolated Perfused Heart System for Small Rodents

The IH-SR Langendorff Core System is the starting point for all isolated perfused heart experiments on small rodents such as mouse, rat and guinea pig in the Langendorff retrograde perfusion mode.

The core Langendorff system can be easily upgraded to a working heart-ready (ejecting heart) configuration any time later.

Because of its larger heart chamber, this system is well-suited for applications that require ECG and MAP recordings in addition to LVP and pacing. The fully closing heart chamber allows for precise temperature control, making it the optimal choice for mouse heart perfusion. 

Please Note: A fully functional IH-SR system requires the addition of core system options and other components. Other options and upgrades enable specific measurements or applications. Please see the Full Description for details.

To ensure that your system is properly configured as a functional unit that meets your application needs, please complete the Checklist at the back of the catalog and then contact Technical Services before placing an order. In the US and outside of Europe, call 800-547-6766 or email support@hbiosci.com. In Europe, please call (49) 7665-92000 or email sales@hugo-sachs.de.

Item# Description U.S. List Price Quantity
73-4343 IH-SR Core System for Isolated Small Rodent Heart, 230 VAC
73-4344 IH-SR Core System for Isolated Small Rodent Heart, 115 VAC
Login for U.S. Pricing


The IH-SR Langendorff Core System is the starting point for all isolated perfused heart experiments on small rodents such as mouse, rat and guinea pig in the Langendorff retrograde perfusion mode.

The Core System contains all the primary equipment you need to accomplish the basic Langendorff experiment. A fully functional IH-SR Langendorff System additionally requires the selection of core system options and other components including:

  • Method of constant pressure perfusion
  • Species-specific additions (cannulae, buffer reservoirs and balloons)
  • Desired data acquisition system

The IH-SR Langendorff Core System can be upgraded to a fully ejecting working heart system (according to Neely), accommodating aortic flows up to 60 ml/min. Other options enable additional measurement and application-specific capabilities.

Advanced System Design
Features & Benefits
Applications
Measured Signals & Calculated Parameters
Included items
Additional Requirements for a Functional Langendorff Unit
Additional Components
Specialized Applications & Upgrades


Advanced System Design

Advanced design provides ease-of-use and exceptional stability of measurements while maintaining the flexibility to upgrade to more advanced capabilities. 

IH series systems do not use high water-columns to achieve a constant pressure and rely on instead other safer methods (Starling resistor or feedback-based perfusion controller) that keep every portion of your system within easy reach.

The aortic block is fully enclosed in the water-jacketed upper and lower IH-SR heart chamber and precision milled from thermally stable Perspex. This design allows for precise temperature control and stability, making it the optimal choice for mouse heart perfusion. Because of its larger heart chamber, this system is well-suited for applications that require ECG and MAP recordings in addition to LVP and pacing.

Back to Top


Features & Benefits

  • Langendorff and optional working heart perfusion in a single system
  • Compact design with no high water columns—optimized individually for mouse, rat or guinea pig
  • Constant pressure or constant flow perfusion in one unit—easily switch between the two modes without changes in tubing setup
  • Suitable for hearts from hypertensive rats (perfusion pressures up to 300 mmHg are possible)
  • Unique integrated small volume aortic block with built-in bubble trap (immediately above the aortic cannula) and Windkessel
  • Large fully-heated, closed heart chamber—important to maintain heart temperature
    • Natural physiological environment for the isolated heart
    • Hearts are kept alive for hours in a very stable physiologic environment
  • All electrodes, catheters and probes are fully enclosed  in the unique closed jacketed chamber—easy, direct access to the heart and to maintain physiological conditions while performing the experiments
  • Low dead space volume—fluid injection pathway directly on top of the aortic cannula into aortic stream
  • Different cannula  sizes available—all metal, no fragile glass cannulae
  • Cannula resistance is optimized according to Hagen-Poiseuilles physical law
  • Proprietary mini-holders—allow easy and stress-free positioning of electrodes, catheters and probes

Back to Top


Applications

  • Study of myogene autoregulation with the addition of Direct Coronary Flow Measurement Option    
  • Testing with inotropic substances
  • Testing of lusitrope substances
  • Testing of vasoactive substances
  • Cardiac rhythm tests
  • Ischemia/hypoxia studies
  • Refractory period studies
  • Ischemia/reperfusion injury studies
  • Cardioplegia studies
  • Cardiac preconditioning
  • Cardiovascular screening performance
  • Electrophysiology studies
  • Phenotyping of transgenic animals 
  • Drug compound screening
  • Toxicology studies
  • Biochemical tests

Back to Top


Measured Signals and Calculated Parameters

 The following signals are recorded as raw data in retrograde Langendorff perfusion:

  • Isovolumetric left ventricular pressure (LVP) with balloon method
  • Aortic (perfusion) pressure
  • Coronary flow*

The following parameters are calculated from the raw data (using the ISOHEART data acquisition software):

  • dLVP/dt, dLVP/dt Max, dLVP/dt Min, contractility index
  • Systolic and diastolic LVP
  • Heart rate
  • Mean perfusion pressure
  • Mean perfusion flow*
  • Coronary resistance*

* This parameter is based on indirect flow measurement with the SCP controller or direct ultrasound flow measurement with the TTFM-2 flowmeter and suitable flow probe.

Back to Top


Included Items

Included items are representative of a typical IH-SR Core System. Individual components can be customized to your needs.

IH-SR Core System, Basic, 230 V (73-4343) includes: IH-SR Core System, Basic, 115 V (73-4344) includes:
73-4991 IH-SR Base Unit for Small Rodent Hearts (Up to 800 g) 73-4991 IH-SR Base Unit for Small Rodent Hearts (Up to 800 g)
73-4544 Thermostatic Circulator TC120-ST5, Bath Volume 5 L, 230 V/50 Hz 73-4545 Thermostatic Circulator TC120-ST5, Bath Volume 5 L, 115 V/60 Hz
73-2448 REGLO Analog -Channel Peristaltic Pump, 230 VAC, 60 Hz
73-2952 REGLO Analog 2 Channel Peristaltic Pump, 115 VAC, 50 Hz
73-0045 PLUGSYS Case, Type 603 73-0045 PLUGSYS Case, Type 603
73-1793 PLUGSYS Transducer Amplifier Module (TAM-D) 73-1793 PLUGSYS Transducer Amplifier Module (TAM-D)
73-0065 PLUGSYS Transducer Amplifier Module (TAM-A) (Does not include balloon) 73-0065 PLUGSYS Transducer Amplifier Module (TAM-A) (Does not include balloon)
73-3862 APT300 Pressure Transducers (2 included) 73-3862 APT300 Pressure Transducers (2 included)
73-3869 Holder for APT300 Transducer, 8 mm Rod, Length 75 mm 73-3869 Holder for APT300 Transducer, 8 mm Rod, Length 75 mm
73-0566 Perspex Block Clamp to mount transducer onto rod (up to 9 mm OD) 73-0566 Perspex Block Clamp to mount transducer onto rod (up to 9 mm OD)

 

The IH-SR Base Unit (73-4991) includes:

Plexiglas stand, aortic block with aorta link unit with Windkessel, heat exchanger, jacketed heart chamber, needle valve for adjusting gas flow, holder for transducer for perfusion pressure, tubings. Aortic cannula and buffer reservoir must be ordered separately.

For a functional unit, species-specific core options and pressure controller systems must be added.

Back to Top


Additional Requirements for a Functional Langendorff Unit

An IH-SR Langendorff basic functional unit requires the addition of core options to the selected Core System (either 73-4343 or 73-4344), specifically, the addition of a perfusion pressure controllers (SCP module or Starling resistor) and a species-specific addition.

Back to Top


Additional Components

Please contact our technical team for help.

ISOHEART Data Acquisition Software

Provides real-time evaluation of a wide range of signals and classical cardiovascular parameters. 

Note: Ponemah Data Acquisition & Analysis Software from DSI,a Harvard Bioscience Company is also suitable. 

Thermocirculator

Used to warm and maintain temperature of the perfusate. 

Buffer Reservoirs

Used in conjunction with a peristaltic pump to deliver warmed perfusate to the target organ and to aerate the perfusate with O2 and CO2.

Back to Top


Specialized Applications & Upgrades

IH-SR Core System Options

Options for required additions to the IH-SR Core System for species-specific Langendorff Only or Langendorff Working Heart-Ready configurations.

Working Heart Upgrade

The IH-SR Langendorff Core System is prepared for upgrade to a working ejecting heart  according to Neely later on. To upgrade an IH-SR Lagendorff Core System to a IH-SR Working Heart System, add the Working Heart Upgrade and one of the species-specific additions. Researchers who know they will be upgrading to working ejecting heart in the future may start with a Langendorff Working Heart-Ready configuration. If you intend to measure coronary flow, you need a TTFM-2 flowmeter and a species suitable flowmeter. (SCP cannot be used for Working Heart.)

Langendorff Only configuration can easily be modified to a Langendorff Working Heart-Ready configuration. Please contact our technical team for help!

Coronary Flow Measurement Option 

Coronary flow measurement can be accomplished in an IH-SR unit using two methods: 

  • Indirect measurement by controlling the pump sped of a roller pump (SCP controller)
  • Direct measurement with Ultrasound Transit Time Technic (TTFM-2 module)

Coronary Effluent Collection Option

Pulmonary artery cannulae, preparation dish and effluate funnel for collection of effluate from the coronaries for metabolic studies, further analysis, or forcontinuous pO2,pH, or pCO2 measurements. 

Perfusate Oxygenation Option

For oxygenation of buffer solution supplemented with albumin, fatty acids, washed erythrocytes, or other foaming additives.

Perfusate Filtration Option

For filtraion of recirculated perfusate.

Temperature Measurement Option

Measure perfusate temperature in any isolated perfused organ system. Since the system is well thermostated, temperature measurement is only necessary when specific temperature studies with special temperature protocols are being performed, e.g. cooling or transplantation studies.

Perfusion Solution Monitoring

Permits precise continuous or intermittent measurement in liquid media or perfusate of three key parameters: pO2, pH and pCO2.

Drug Addition Option

These options for drug addition can be added to any isolated organ system where flow is measured or calculated and a drug must be added in a certain ratio.

Cell Isolation Upgrade for IH-SR

Add this upgrade to a working IH-SR system for cell isolation/cell extraction applications.

Pacing Option

For addition of pacing to functional IH-SR or IH-5 Langendorff and Working Heart Systems. For small rodent hearts (mouse, rat and guinea pig). 

Single-Lead ECG and MAP Measurement Options

For single-lead ECG and MAP on functional IH-5 systems, one ECG lead is combined with one MAP electrode.

Back to Top

Specifications